NDS27 combines the effect of curcumin lysinate and hydroxypropyl-β-cyclodextrin to inhibit equine PKCδ and NADPH oxidase involved in the oxidative burst of neutrophils
Publication date: Available online 18 November 2014 Source:FEBS Open Bio Author(s): Sandrine Derochette , Ange Mouithys-Mickalad , Thierry Franck , Simon Collienne , Justine Ceusters , Ginette Deby-Dupont , Philippe Neven , Didier Serteyn Polymorphonuclear neutrophils (PMNs) are involved in host defence against infections by the production of reactive oxygen species (ROS), but excessive PMN stimulation is associated with the development of inflammatory diseases. After appropriate stimuli, protein kinase C (PKC) triggers the assembly of NADPH oxidase (Nox2) which produces superoxide anion (O2 ●-), from which R...
Source: FEBS Open Bio - November 20, 2014 Category: Molecular Biology Source Type: research
Inhibition of autophagy induces retinal pigment epithelial cell damage by the lipofuscin fluorophore A2E
In this study, we show augmented autophagy in the retinal pigment epithelial cell line ARPE-19 when cultured in the presence of the lipofuscin pigment A2E. A2E alone does not induce RPE cell death, but cell death was induced in the presence of A2E with the autophagy inhibitor 3-methyladenine (3MA), with a concomitant increase in the generation of mitochondrial reactive oxygen species. On the other hand, the ATP production capacity of mitochondria was decreased in the presence of A2E, and pharmacological inhibition of autophagy had no additional effects. The altered mRNA expression level of mitochondrial function markers wa...
Source: FEBS Open Bio - November 15, 2014 Category: Molecular Biology Source Type: research
Anticancer drug mithramycin interacts with core histones: An additional mode of action of the DNA groove binder
Publication date: Available online 16 October 2014 Source:FEBS Open Bio Author(s): Amrita Banerjee , Sulagna Sanyal , Kirti K. Kulkarni , Kuladip Jana , Siddhartha Roy , Chandrima Das , Dipak Dasgupta Mithramycin (MTR) is a clinically approved DNA-binding antitumor antibiotic currently in Phase 2 clinical trials at National Institutes of Health for treatment of osteosarcoma. In view of the resurgence in the studies of this generic antibiotic as a human medicine, we have examined the binding properties of MTR with the integral component of chromatin – histone proteins – as a part of our broad objective to cla...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Targeted disruption of the heat shock protein 20–phosphodiesterase 4D (PDE4D) interaction protects against pathological cardiac remodelling in a mouse model of hypertrophy
This study demonstrates that disruption of the specific HSP20–PDE4D interaction leads to attenuation of pathological cardiac remodelling. (Source: FEBS Open Bio)
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
MycN promotes TRPM7 expression and cell migration in neuroblastoma through a process that involves polyamines
The objective of the current study was to elucidate the mechanism by which MycN promotes NB cell migration and the mechanism regulating TRPM7 expression. The results showed that MycN increased TRPM7 expression, induced TRPM7 channel activity, increased intracellular Ca2+ signaling, and promoted cell migration in NB cells. The results also showed that inhibition or down-regulation of ornithine decarboxylase (ODC) inhibited TRPM7 expression, a process that was reversed by spermidine. Overall, this study provides evidence that MycN promotes TRPM7 expression and cell migration through a mechanism that involves ODC synthesis of...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
High-fat diet-induced changes in liver thioredoxin and thioredoxin reductase as a novel feature of insulin resistance
Publication date: Available online 31 October 2014 Source:FEBS Open Bio Author(s): Huijun Qin , Xiaolin Zhang , Fei Ye , Liangwei Zhong High-fat diet (HFD) can induce oxidative stress. Thioredoxin (Trx) and thioredoxin reductase (TrxR) are critical antioxidant proteins but how they are affected by HFD remains unclear. Using HFD-induced insulin-resistant mouse model, we show here that liver Trx and TrxR are significantly decreased, but, remarkably, the degree of their S-acylation is increased after consuming HFD. These HFD-induced changes in Trx/TrxR may reflect abnormalities of lipid metabolism and insulin signalin...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Heart-type fatty-acid-binding protein (FABP3) is a lysophosphatidic acid-binding protein in human coronary artery endothelial cells
In this study, using lysophosphatidic acid (LPA)-coated agarose beads, we have identified FABP3 as an LPA carrier protein in human coronary artery endothelial cells (HCAECs). Administration of LPA to HCAECs resulted in a dose-dependent increase in PPARγ activation. Furthermore, the LPA-induced PPARγ activation was abolished when the FABP3 expression was reduced using small interfering RNA (siRNA). We further show that the nuclear fraction of control HCAECs contained a significant amount of exogenously added LPA, whereas FABP3 siRNA-transfected HCAECs had a decreased level of LPA in the nucleus. Taken together, these resu...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Structure and stability of metagenome-derived glycoside hydrolase family 12 cellulase (LC-CelA) a homolog of Cel12A from Rhodothermus marinus
Publication date: Available online 31 October 2014 Source:FEBS Open Bio Author(s): Hiroyuki Okano , Masashi Ozaki , Eiko Kanaya , Joong-Jae Kim , Clement Angkawidjaja , Yuichi Koga , Shigenori Kanaya Ten genes encoding novel cellulases with putative signal peptides at the N-terminus, termed pre-LC-CelA–J, were isolated from a fosmid library of a leaf–branch compost metagenome by functional screening using agar plates containing carboxymethyl cellulose and trypan blue. All the cellulases except pre-LC-CelG have a 14–29 residue long flexible linker (FL) between the signal peptide and the catalytic domain. LC...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma
Publication date: Available online 4 November 2014 Source:FEBS Open Bio Author(s): Alessandra Mangolini , Anna Bonon , Stefano Volinia , Giovanni Lanza , Roberto Gambari , Paolo Pinton , Gian Rosario Russo , Laura del Senno , Lucio Dell’Atti , Gianluca Aguiari Renal cell carcinoma is a common neoplasia of the adult kidney that accounts for about 3% of adult malignancies. Clear cell renal carcinoma is the most frequent subtype of kidney cancer and 20–40% of patients develop metastases. The absence of appropriate biomarkers complicates diagnosis and prognosis of this disease. In this regard, small noncoding...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex
Publication date: Available online 8 November 2014 Source:FEBS Open Bio Author(s): Sara Vicente-Muñoz , Paco Romero , Lorena Magraner-Pardo , Celia P. Martinez-Jimenez , Vicente Tordera , Mercè Pamblanco Histone acetylation affects several aspects of gene regulation, from chromatin remodelling to gene expression, by modulating the interplay between chromatin and key transcriptional regulators. The exact molecular mechanism underlying acetylation patterns and crosstalk with other epigenetic modifications requires further investigation. In budding yeast, these epigenetic markers are produced partly by histone ace...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382
Publication date: Available online 12 November 2014 Source:FEBS Open Bio Author(s): Jelle B. Bultema , Bas J.H. Kuipers , Lubbert Dijkhuizen The Bacillus circulans ATCC 31382 β-galactosidase (BgaD) is a retaining-type glycosidase of glycoside hydrolase family 2 (GH2). Its commercial enzyme preparation, Biolacta N5, is used for commercial-scale production of galacto-oligosaccharides (GOS). The BgaD active site and catalytic amino acid residues have not been studied. Using bioinformatic routines we identified two putative catalytic glutamates and two highly conserved active site histidines. The site-directed mutants ...
Source: FEBS Open Bio - November 14, 2014 Category: Molecular Biology Source Type: research
Interaction of Sesbania mosaic virus (SeMV) RNA-dependent RNA polymerase (RdRp) with the p10 domain of polyprotein 2a and its implications in SeMV replication
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Kunduri Govind , Arindam Bakshi , Handanahal S. Savithri Identification of viral encoded proteins that interact with RNA-dependent RNA polymerase (RdRp) is an important step towards unraveling the mechanism of replication. Sesbania mosaic virus (SeMV) RdRp was shown to interact strongly with p10 domain of polyprotein 2a and moderately with the protease domain. Mutational analysis suggested that the C-terminal disordered domain of RdRp is involved in the interaction with p10. Coexpression of full length RdRp and p10 resulted in formation of RdRp–p10 c...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Staphylococcal nuclease domain containing-1 (SND1) promotes migration and invasion via angiotensin II type 1 receptor (AT1R) and TGFβ signaling
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Prasanna K. Santhekadur , Maaged Akiel , Luni Emdad , Rachel Gredler , Jyoti Srivastava , Devaraja Rajasekaran , Chadia L. Robertson , Nitai D. Mukhopadhyay , Paul B. Fisher , Devanand Sarkar Staphylococcal nuclease domain containing-1 (SND1) is overexpressed in human hepatocellular carcinoma (HCC) patients and promotes tumorigenesis by human HCC cells. We now document that SND1 increases angiotensin II type 1 receptor (AT1R) levels by increasing AT1R mRNA stability. This results in activation of ERK, Smad2 and subsequently the TGFβ signaling p...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Kohei Yamashita , Yuichiro Hagiya , Motowo Nakajima , Masahiro Ishizuka , Tohru Tanaka , Shun-ichiro Ogura The nuclear receptor, REV-ERBα, has a key role in circadian rhythms and requires heme as its ligand. The present study determined whether the heme precursor, 5-aminolevulinic acid (ALA), affects REV-ERBα and its target genes. When exposed to ALA, the human lung diploid cell line, WI-38, exhibited activation of REV-ERBα and repression of the transcription of REV-ERBα target genes, including BMAL1, an essential component of the circadian osci...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Takaaki Hiragun , Yuhki Yanase , Tsutomu Okabe , Makiko Hiragun , Mikio Kawai , Michihiro Hide Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth signals. Moreover, NCL-2 cells expressed FcεR...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
