NFκB mediates cisplatin resistance through histone modifications in head and neck squamous cell carcinoma (HNSCC)
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Luciana O. Almeida , Aline C. Abrahao , Luciana K. Rosselli-Murai , Fernanda S. Giudice , Chiara Zagni , Andreia M. Leopoldino , Cristiane H. Squarize , Rogerio M. Castilho Cisplatin-based chemotherapy is the standard treatment of choice for head and neck squamous cell carcinoma (HNSCC). The efficiency of platinum-based therapies is directly influenced by the development of tumor resistance. Multiple signaling pathways have been linked to tumor resistance, including activation of nuclear factor kappa B (NFκB). We explore a novel mechanism by whic...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Control of reactive oxygen species (ROS) production through histidine kinases in Aspergillus nidulans under different growth conditions
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Saki Hayashi , Megumi Yoshioka , Tetsuji Matsui , Kensuke Kojima , Masashi Kato , Kyoko Kanamaru , Tetsuo Kobayashi Sensor histidine kinases (HKs) are important factors that control cellular growth in response to environmental conditions. The expression of 15 HKs from Aspergillus nidulans was analyzed by quantitative real-time PCR under vegetative, asexual, and sexual growth conditions. Most HKs were highly expressed during asexual growth. All HK gene-disrupted strains produced reactive oxygen species (ROS). Three HKs are involved in the control of...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Gajendra Kumar Azad , Vikash Singh , Papita Mandal , Prabhat Singh , Upendarrao Golla , Shivani Baranwal , Sakshi Chauhan , Raghuvir S. Tomar Ebselen is a synthetic, lipid-soluble seleno-organic compound. The high electrophilicity of ebselen enables it to react with multiple cysteine residues of various proteins. Despite extensive research on ebselen, its target molecules and mechanism of action remains less understood. We performed biochemical as well as in vivo experiments employing budding yeast as a model organism to understand the mode of a...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Functional characterization of WalRK: A two-component signal transduction system from Bacillus anthracis
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Alisha Dhiman , Sonika Bhatnagar , Parul Kulshreshtha , Rakesh Bhatnagar Two-component signal transduction systems (TCS), consisting of a sensor histidine protein kinase and its cognate response regulator, are an important mode of environmental sensing in bacteria. Additionally, they have been found to regulate virulence determinants in several pathogens. Bacillus anthracis, the causative agent of anthrax and a bioterrorism agent, harbours 41 pairs of TCS. However, their role in its pathogenicity has remained largely unexplored. Here, we show that Wal...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Trim27-deficient mice are susceptible to streptozotocin-induced diabetes
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Mohammad Mahabub-Uz Zaman , Toshie Shinagawa , Shunsuke Ishii Tumor necrosis factor α (TNF-α) plays an important role in cell proliferation and apoptosis, and defects in TNF-α-induced apoptosis are associated with various diseases. TRIM27 is a tripartite motif (TRIM) protein containing RING finger, B-box, and coiled-coil domains. We recently reported that TRIM27 positively regulates TNF-α-induced apoptosis through deubiquitination of receptor-interacting protein 1 (RIP1). Multiple studies have suggested a link between TNF-α pathway and various dis...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): C. Massart , N. Giusti , R. Beauwens , J.E. Dumont , F. Miot , J. Van Sande NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2 .− and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activated the efflux of radioiodide but not of its analog 99mTcO4 − nor of the K+ cation mimic 86Rb+ in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expres...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Quantitation of fibroblast activation protein (FAP)-specific protease activity in mouse, baboon and human fluids and organs
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Fiona M. Keane , Tsun-Wen Yao , Stefanie Seelk , Margaret G. Gall , Sumaiya Chowdhury , Sarah E. Poplawski , Jack H. Lai , Youhua Li , Wengen Wu , Penny Farrell , Ana Julia Vieira de Ribeiro , Brenna Osborne , Denise M.T. Yu , Devanshi Seth , Khairunnessa Rahman , Paul Haber , A. Kemal Topaloglu , Chuanmin Wang , Sally Thomson , Annemarie Hennessy , John Prins , Stephen M. Twigg , Susan V. McLennan , Geoffrey W. McCaughan , William W. Bachovchin , Mark D. Gorrell The protease fibroblast activation protein (FAP) is a specific mark...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
The E144 residue of Scherffelia dubia centrin discriminates between the DNA repair protein XPC and the centrosomal protein Sfi1
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Dora Grecu , Yves Blouquit , Liliane Assairi Centrins are members of the EF-hand family of calcium-binding proteins, which are highly conserved among eukaryotes. Centrins bind to several cellular targets, through a hydrophobic triad. However, the W1xxL4xxxL8 triad in XPC (Xeroderma Pigmentosum Group C protein) is found in the reverse orientation, as in the L8xxxL4xxW1 triad in Sfi1 (Suppressor of Fermentation-Induced loss of stress resistance protein 1). As shown by previous NMR studies of human centrin 2 in complex with XPC or Sfi1, the E148 residue o...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Hyun Uk Kim , Su-Jin Jung , Kyeong-Ryeol Lee , Eun Ha Kim , Sang-Min Lee , Kyung Hee Roh , Jong-Bum Kim The LEAFY COTYLEDON2 (LEC2) gene plays critically important regulatory roles during both early and late embryonic development. Here, we report the identification of the LEC2 gene from the castor bean plant (Ricinus communis), and characterize the effects of its overexpression on gene regulation and lipid metabolism in transgenic Arabidopsis plants. LEC2 exists as a single-copy gene in castor bean, is expressed predominantly in embryos, and encode...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Mast cell deficiency results in the accumulation of preadipocytes in adipose tissue in both obese and non-obese mice
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Yasushi Ishijima , Shin’ya Ohmori , Kinuko Ohneda Mast cells have been suggested to play key roles in adipogenesis. We herein show that the expression of preadipocyte, but not adipocyte, marker genes increases in the white adipose tissue of mast cell-deficient (KitW-sh/W-sh ) mice under both obese and non-obese conditions. In vitro culturing with adipogenic factors revealed increased adipocytes differentiated from the KitW-sh/W-sh stromal vascular fraction, suggesting the accumulation of preadipocytes. Moreover, the increased expression of preadipoc...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Stephanie Kern , Han-Zhong Feng , Hongguang Wei , Steven Cala , J.-P. Jin We previously reported that a restrictive N-terminal truncation of cardiac troponin I (cTnI-ND) is up-regulated in the heart in adaptation to hemodynamic stresses. Over-expression of cTnI-ND in the hearts of transgenic mice revealed functional benefits such as increased relaxation and myocardial compliance. In the present study, we investigated the subsequent effect on myocardial remodeling. The alpha-smooth muscle actin (α-SMA) isoform is normally expressed in differentiating...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons
Publication date: 2014 Source:FEBS Open Bio, Volume 4 Author(s): Daiyu Honda , Shinsuke Ishigaki , Yohei Iguchi , Yusuke Fujioka , Tsuyoshi Udagawa , Akio Masuda , Kinji Ohno , Masahisa Katsuno , Gen Sobue TDP-43 and FUS are linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), and loss of function of either protein contributes to these neurodegenerative conditions. To elucidate the TDP-43- and FUS-regulated pathophysiological RNA metabolism cascades, we assessed the differential gene expression and alternative splicing profiles related to regulation by either TDP-43 or FU...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
An alternative allosteric regulation mechanism of an acidophilic l-lactate dehydrogenase from Enterococcus mundtii 15-1A
Publication date: Available online 6 September 2014 Source:FEBS Open Bio Author(s): Yasuyuki Matoba , Masashi Miyasako , Koichi Matsuo , Kosuke Oda , Masafumi Noda , Fumiko Higashikawa , Takanori Kumagai , Masanori Sugiyama A plant-derived Enterococcus mundtii 15-1A, that has been previously isolated from Brassica rapa L. subsp. nipposinica (L.H. Bailey) Hanelt var. linearifolia by our group, possesses two kinds of l-lactate dehydrogenase (l-LDH): LDH-1 and LDH-2. LDH-1 was activated under low concentration of fluctose-1,6-bisphosphate (FBP) at both pH 5.5 and 7.5. Although LDH-2 was also activated under the lo...
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Characterization of the shrimp neuroparsin (MeNPLP): RNAi silencing resulted in inhibition of vitellogenesis
In conclusion, this study has provided the first evidence that MeNPLP is involved in the initial stage of ovary maturation in shrimp. (Source: FEBS Open Bio)
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
Porcine SLITRK1: Molecular cloning and characterization
Publication date: Available online 7 October 2014 Source:FEBS Open Bio Author(s): Knud Larsen , Jamal Momeni , Leila Farajzadeh , Christian Bendixen The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks introns, as does its human and mouse counterparts. RT-PCR cloning revealed two SLITRK1 transcripts: a full-length mRNA and a transcript variant that results in a truncated protein....
Source: FEBS Open Bio - October 12, 2014 Category: Molecular Biology Source Type: research
