Functional characterization of a BCL10 isoform in the rainbow trout Oncorhynchus mykiss
Publication date: Available online 4 February 2015 Source:FEBS Open Bio Author(s): Pellegrino Mazzone , Ivan Scudiero , Elena Coccia , Angela Ferravante , Marina Paolucci , Egildo Luca D’Andrea , Ettore Varricchio , Maddalena Pizzulo , Carla Reale , Tiziana Zotti , Pasquale Vito , Romania Stilo The complexes formed by BCL10, MALT1 and members of the family of CARMA proteins have recently been the focus of much attention because they represent a key mechanism for regulating activation of the transcription factor NF-κB. Here, we report the functional characterization of a novel isoform of BCL10 in the trou...
Source: FEBS Open Bio - February 5, 2015 Category: Molecular Biology Source Type: research
The SH3 regulatory domain of the hematopoietic cell kinase Hck binds ELMO via its polyproline motif
Publication date: Available online 4 February 2015 Source:FEBS Open Bio Author(s): Awad Rida , Sévajol Marion , Ayala Isabel , Chouquet Anne , Frachet Philippe , Gans Pierre , Reiser Jean-Baptiste , Kleman Jean-Philippe Eukaryotic EnguLfment and cell MOtility (ELMO) proteins form an evolutionary conserved family of regulators involved in small GTPase dependent actin remodeling processes that regulates the guanine exchange factor activity of some of the DOwnstream of CrK (DOCK) family members. Gathered data strongly suggest that DOCK activation by ELMO and the subsequent signaling result from a subtle balance i...
Source: FEBS Open Bio - February 5, 2015 Category: Molecular Biology Source Type: research
MutT from the fish pathogen Aliivibrio salmonicida is a cold-active nucleotide-pool sanitization enzyme with unexpectedly high thermostability
Publication date: Available online 3 February 2015 Source:FEBS Open Bio Author(s): Kjersti Lian , Hanna-Kirsti S. Leiros , Elin Moe Upon infection by pathogenic bacteria, the production of reactive oxygen species (ROS) is part of the host organism’s first line of defence. ROS damage a number of macromolecules, and in order to withstand such a harsh environment, the bacteria need to have well-functioning ROS scavenging and repair systems. Herein, MutT is an important nucleotide-pool sanitization enzyme, which degrades 8-oxo-dGTP and thus prevents it from being incorporated into DNA. In this context, we have perform...
Source: FEBS Open Bio - February 4, 2015 Category: Molecular Biology Source Type: research
Friend or Foe: endoplasmic reticulum protein 29 (ERp29) in epithelial cancer
Publication date: Available online 30 January 2015 Source:FEBS Open Bio Author(s): Shaohua Chen , Daohai Zhang The endoplasmic reticulum (ER) protein 29 (ERp29) is a molecular chaperone that plays a critical role in protein secretion from the ER in eukaryotic cells. Recent studies have also shown that ERp29 plays a role in cancer. It has been demonstrated that ERp29 is inversely associated with primary tumor development and functions as a tumor suppressor by inducing cell growth arrest in breast cancer. However, ERp29 has also been reported to promote epithelial cell morphogenesis, cell survival against genotoxic str...
Source: FEBS Open Bio - January 30, 2015 Category: Molecular Biology Source Type: research
Paired image- and FACS-based toxicity assays for high content screening of spheroid-type tumor cell cultures
Publication date: Available online 28 January 2015 Source:FEBS Open Bio Author(s): Kari Trumpi , David A. Egan , Thomas T. Vellinga , Inne H.M. Borel Rinkes , Onno Kranenburg Novel spheroid-type tumor cell cultures directly isolated from patients’ tumors preserve tumor characteristics better than traditionally grown cell lines. However, such cultures are not generally used for high-throughput toxicity drug screens. In addition, the assays that are commonly used to assess drug-induced toxicity in such screens usually measure a proxy for cell viability such as mitochondrial activity or ATP-content per culture well...
Source: FEBS Open Bio - January 29, 2015 Category: Molecular Biology Source Type: research
Ligand binding specificity of RutR, a member of the TetR family of transcription regulators in Escherichia coli
Publication date: Available online 28 January 2015 Source:FEBS Open Bio Author(s): Phu Nguyen Le Minh , Sergio de Cima , Indra Bervoets , Dominique Maes , Vicente Rubio , Daniel Charlier RutR is a member of the large family of TetR transcriptional regulators in E. coli. It was originally discovered as the regulator of the rutABCDEFG operon encoding a novel pathway for pyrimidine utilization, but its highest affinity target is the control region of the carAB operon, encoding carbamoylphosphate synthase. Unlike most other TetR-like regulators, RutR exerts both positive and negative effects on promoter activity. Fur...
Source: FEBS Open Bio - January 29, 2015 Category: Molecular Biology Source Type: research
Distinct conformational and functional effects of two adjacent pathogenic mutations in cardiac troponin I at the interface with troponin T
Publication date: Available online 13 January 2015 Source:FEBS Open Bio Author(s): Shirin Akhter , J.-P. Jin The α-helix in troponin I (TnI) at the interface with troponin T (TnT) is a highly conserved structure. A point mutation in this region, A116G, was found in human cardiac TnI in a case of cardiomyopathy. An adjacent dominantly negative mutation found in turkey cardiac TnI (K111C, equivalent to K117C in human and K118C in mouse) decreased diastolic function and blunted beta-adrenergic response in transgenic mice. To investigate the functional importance of the TnI-TnT interface and pathological impact of the c...
Source: FEBS Open Bio - January 13, 2015 Category: Molecular Biology Source Type: research
Diffuse binding of Zn2+ to the denatured ensemble of Cu/Zn superoxide dismutase 1
In this study, we have examined at residue level the nature of this early Zn2+ binding by NMR studies on the urea denatured-state of SOD1. Nearly complete backbone chemical shift assignments were obtained in 9 M urea at physiological pH, conditions at which NMR studies are scarce. Our results demonstrate that SOD1 is predominantly unstructured under these conditions. Chemical-shift changes upon Zn2+ titration show that denatured SOD1 retains a significant affinity to Zn2+ ions, even in 9 M urea. However, the Zn2+ interactions are not limited to the native metal-binding ligands in the two binding sites, but are seen for all...
Source: FEBS Open Bio - January 3, 2015 Category: Molecular Biology Source Type: research
Soluble cysteine-rich tick saliva proteins Salp15 and Iric-1 from E. coli
Publication date: Available online 24 December 2014 Source:FEBS Open Bio Author(s): Philipp Kolb , Jolanta Vorreiter , Jüri Habicht , Detlef Bentrop , Reinhard Wallich , Michael Nassal Ticks transmit numerous pathogens, including borreliae, which cause Lyme disease. Tick saliva contains a complex mix of anti-host defense factors, including the immunosuppressive cysteine-rich secretory glycoprotein Salp15 from Ixodes scapularis ticks and orthologs like Iric-1 from I. ricinus. All tick-borne microbes benefit from the immunosuppression at the tick bite site; in addition, borreliae exploit the binding of Salp15 to t...
Source: FEBS Open Bio - December 25, 2014 Category: Molecular Biology Source Type: research
Role of histamine H3 receptor in glucagon-secreting αTC1.6 cells
Publication date: Available online 13 December 2014 Source:FEBS Open Bio Author(s): Tadaho Nakamura , Takeo Yoshikawa , Fumito Naganuma , Attayeb Mohsen , Tomomitsu Iida , Yamato Miura , Akira Sugawara , Kazuhiko Yanai Pancreatic α-cells secrete glucagon to maintain energy homeostasis. Although histamine has an important role in energy homeostasis, the expression and function of histamine receptors in pancreatic α-cells remains unknown. We found that the histamine H3 receptor (H3R) was expressed in mouse pancreatic α-cells and αTC1.6 cells, a mouse pancreatic α-cell line. H3R inhibited glucagon secretion f...
Source: FEBS Open Bio - December 14, 2014 Category: Molecular Biology Source Type: research
Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos
Publication date: Available online 3 December 2014 Source:FEBS Open Bio Author(s): Christina Brandl , Oskar Ortiz , Bernhard Röttig , Benedikt Wefers , Wolfgang Wurst , Ralf Kühn The use of TALEN and CRISPR/CAS nucleases is becoming increasingly popular as a means to edit single target sites in one-cell mouse embryos. Nevertheless, an area that has received less attention concerns the engineering of structural genome variants and the necessary religation of two distant double-strand breaks. Herein, we applied pairs of TALEN or sgRNAs and Cas9 to create deletions in the Rab38 gene. We found that the deletion of ...
Source: FEBS Open Bio - December 4, 2014 Category: Molecular Biology Source Type: research
Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element-binding protein) was down-regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC-MS/MS. Pretreatment with piperine decreased eugenol-induced cAMP and calcium levels in 3T3-L1...
Source: FEBS Open Bio - November 28, 2014 Category: Molecular Biology Source Type: research
Functional classification and biochemical characterization of a novel rho class glutathione S-transferase in Synechocystis PCC 6803
We report a novel class of glutathione S-transferase (GST) from the model cyanobacterium Synechocystis PCC 6803 (sll1545) which catalyzes the detoxification of the water pollutant dichloroacetate and also shows strong glutathione-dependent peroxidase activity representing the classical activities of zeta and theta/alpha class respectively. Interestingly, sll1545 has very low sequence and structural similarity with these classes. This is the first report of dichloroacetate degradation activity by any bacterial GST. Based on these results we classify sll1545 to a novel GST class, rho. The present data also indicate potential...
Source: FEBS Open Bio - November 25, 2014 Category: Molecular Biology Source Type: research
Secretory prostate apoptosis response (Par)-4 sensitizes multicellular spheroids (MCS) of glioblastoma multiforme cells to tamoxifen-induced cell death
Publication date: Available online 21 November 2014 Source:FEBS Open Bio Author(s): Jayashree C. Jagtap , Parveen D , Reecha D. Shah , Aarti Desai , Dipali Bhosale , Ashish Chugh , Deepak Ranade , Swapnil Karnik , Bhushan Khedkar , Aaishwarya Mathur , Kumar Natesh , Goparaju Chandrika , Padma Shastry Glioblastoma multiforme (GBM) is the most malignant form of brain tumor and is associated with resistance to conventional therapy and poor patient survival. Prostate apoptosis response (Par)-4, a tumor suppressor, is expressed as both an intracellular and secretory/extracellular protein. Though secretory Par-4...
Source: FEBS Open Bio - November 22, 2014 Category: Molecular Biology Source Type: research
Corrigendum to “The ALS/FTLD-related RNA-binding proteins TDP-43 and FUS have common downstream RNA targets in cortical neurons”
Publication date: Available online 22 November 2014 Source:FEBS Open Bio Author(s): Daiyu Honda , Shinsuke Ishigaki , Yohei Iguchi , Yusuke Fujioka , Tsuyoshi Udagawa , Akio Masuda , Kinji Ohno , Masahisa Katsuno , Gen Sobue (Source: FEBS Open Bio)
Source: FEBS Open Bio - November 22, 2014 Category: Molecular Biology Source Type: research
