DNMT1 mediates chemosensitivity by reducing methylation of miRNA-20a promoter in glioma cells.
This study investigated whether DNA methyltransferase (DNMT) expression was associated with TMZ sensitivity in glioma cells and elucidated the underlying mechanism. DNMT expression was analyzed by western blotting. miR-20a promoter methylation was evaluated by methylation-specific PCR. Cell viability and apoptosis were assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and TdT-mediated dUTP-biotin nick end labeling assays, respectively. The results showed that compared with parental U251 cells, DNMT1 expression was downregulated, miR-20a promoter methylation was attenuated and miR-20...
Source: exp Mol Med - September 5, 2015 Category: Molecular Biology Authors: Zhou D, Wan Y, Xie D, Wang Y, Wei J, Yan Q, Lu P, Mo L, Xie J, Yang S, Qi X Tags: Exp Mol Med Source Type: research
Transcriptional mutagenesis by 8-oxodG in α-synuclein aggregation and the pathogenesis of Parkinson's disease.
Abstract
Parkinson's disease (PD) is an age-related progressive neurodegenerative disease associated with selective loss of dopaminergic neurons. The characteristic hallmark of the disease is intracytoplasmic proteinacious inclusion bodies called Lewy bodies, primarily consisting of a presynaptic protein α-synuclein. Oxidative stress-mediated damage to macromolecules have been shown to occur frequently in PD. Oxidative damage to DNA in the form of oxidized guanine (8-oxodG) accumulates in both the mitochondrial and nuclear DNA of dopaminergic neurons of the substantia nigra in PD. 8-oxodG-mediated transcr...
Source: exp Mol Med - September 2, 2015 Category: Molecular Biology Authors: Basu S, Je G, Kim YS Tags: Exp Mol Med Source Type: research
The effect of ferulic acid ethyl ester on leptin-induced proliferation and migration of aortic smooth muscle cells.
In conclusion, FAEE exerts multiple effects on leptin-induced cell proliferation and migration, including the inhibition of p44/42MAPK phosphorylation, cell cycle-regulatory proteins and MMP-9, thereby suggesting that FAEE may be a possible therapeutic approach to the inhibition of obese vascular disease.
PMID: 26315599 [PubMed - in process] (Source: exp Mol Med)
Source: exp Mol Med - September 2, 2015 Category: Molecular Biology Authors: Tsai YC, Lee YM, Hsu CH, Leu SY, Chiang HY, Yen MH, Cheng PY Tags: Exp Mol Med Source Type: research
Middle East respiratory syndrome coronavirus: transmission, virology and therapeutic targeting to aid in outbreak control.
In this study, we highlight the potential drug targets that can be used to develop anti-MERS-CoV therapeutics.
PMID: 26315600 [PubMed - in process] (Source: exp Mol Med)
Source: exp Mol Med - September 2, 2015 Category: Molecular Biology Authors: Durai P, Batool M, Shah M, Choi S Tags: Exp Mol Med Source Type: research
miR-526b targets 3' UTR of MMP1 mRNA.
Abstract
Regulation of matrix metalloproteinases (MMPs) is important for many physiological processes involving cancers, inflammation, tissue remodeling and skin aging. Here, we report the novel finding that the expression of MMP1 mRNA is downregulated by the overexpression of miR-526b which is a member of chromosome 19 microRNA cluster (C19MC). Our analysis using reporter constructs containing the 3' untranslated region (3' UTR) of MMP1 and its mutant form showed that the region from 377-383 in the 3' UTR of MMP1 is critical for targeting by miR-526b. In addition, the expression pattern of miR-526b and MM...
Source: exp Mol Med - August 24, 2015 Category: Molecular Biology Authors: Kim KH, Jung JY, Son ED, Shin DW, Noh M, Lee TR Tags: Exp Mol Med Source Type: research
N-acetyl-D-glucosamine kinase interacts with dynein light-chain roadblock type 1 at Golgi outposts in neuronal dendritic branch points.
In this study, we conducted yeast two-hybrid screening to search for NAGK-binding proteins and found a specific interaction between NAGK and dynein light-chain roadblock type 1 (DYNLRB1). Immunocytochemistry (ICC) on hippocampal neurons using antibodies against NAGK and DYNLRB1 or dynein heavy chain showed some colocalization, which was increased by treating the live cells with a crosslinker. A proximity ligation assay (PLA) of NAGK-dynein followed by tubulin ICC showed the localization of PLA signals on microtubule fibers at dendritic branch points. NAGK-dynein PLA combined with Golgi ICC showed the colocalization of PLA ...
Source: exp Mol Med - August 16, 2015 Category: Molecular Biology Authors: Islam MA, Sharif SR, Lee H, Seog DH, Moon IS Tags: Exp Mol Med Source Type: research
Prognostic role of genetic biomarkers in clinical progression of prostate cancer.
Abstract
The aim of this study was to analyze the use of 12 single-nucleotide polymorphisms in genes ELAC2, RNASEL and MSR1 as biomarkers for prostate cancer (PCa) detection and progression, as well as perform a genetic classification of high-risk patients. A cohort of 451 men (235 patients and 216 controls) was studied. We calculated means of regression analysis using clinical values (stage, prostate-specific antigen, Gleason score and progression) in patients and controls at the basal stage and after a follow-up of 72 months. Significantly different allele frequencies between patients and controls were o...
Source: exp Mol Med - August 9, 2015 Category: Molecular Biology Authors: Alvarez-Cubero MJ, Martinez-Gonzalez LJ, Saiz M, Carmona-Saez P, Alvarez JC, Pascual-Geler M, Lorente JA, Cozar JM Tags: Exp Mol Med Source Type: research
The 5-hydroxytryptamine transporter is functional in human coronary artery smooth muscle cells proliferation and is regulated by Interleukin-1 beta.
Abstract
Abnormal human coronary artery smooth muscle cells (hCASMCs) proliferation and migration are key factors in coronary artery restenosis after percutaneous coronary intervention. Platelets release 5-hydroxytryptamine (5-HT), which is a strong mitogen for pulmonary artery smooth muscle cells proliferation and migration. Here, we investigated the effects of 5-HT and role of 5-HT transporter (5-HTT) on hCASMCs proliferation and migration. The 5-HT (10(-6)-10(-5) mol/l) significantly increased hCASMCs proliferation and migration, and these effects were inhibited by fluoxetine (10(-5) mol/l) and citalopr...
Source: exp Mol Med - August 2, 2015 Category: Molecular Biology Authors: Wang QJ, Wang D, Tang CC Tags: Int J Clin Exp Med Source Type: research
RELMα can cause contraction of rat aortic smooth muscle cells.
CONCLUSIONS: RELMα can cause contraction of rat aortic smooth muscle cells, its mechanism may be via Ca(2+)-CaM-MLCK pathway.
PMID: 26221238 [PubMed] (Source: exp Mol Med)
Source: exp Mol Med - August 2, 2015 Category: Molecular Biology Authors: Li X, Duan S, Zhang H, Han S, Jin Q, Cui R Tags: Int J Clin Exp Med Source Type: research
Epigenetic modulation as a therapeutic approach for pulmonary arterial hypertension.
Abstract
Pulmonary arterial hypertension (PAH) is a rare but progressive and currently incurable disease, which is characterized by vascular remodeling in association with muscularization of the arterioles, medial thickening and plexiform lesion formation. Despite our advanced understanding of the pathogenesis of PAH and the recent therapeutic advances, PAH still remains a fatal disease. In addition, the susceptibility to PAH has not yet been adequately explained. Much evidence points to the involvement of epigenetic changes in the pathogenesis of a number of human diseases including cancer, peripheral hyp...
Source: exp Mol Med - August 2, 2015 Category: Molecular Biology Authors: Kim JD, Lee A, Choi J, Park Y, Kang H, Chang W, Lee MS, Kim J Tags: Exp Mol Med Source Type: research
Fetal hematopoietic stem cells express MFG-E8 during mouse embryogenesis.
Abstract
The milk fat globule-EGF-factor 8 protein (MFG-E8) has been identified in various tissues, where it has an important role in intercellular interactions, cellular migration, and neovascularization. Previous studies showed that MFG-E8 is expressed in different cell types under normal and pathophysiological conditions, but its expression in hematopoietic stem cells (HSCs) during hematopoiesis has not been reported. In the present study, we investigated MFG-E8 expression in multiple hematopoietic tissues at different stages of mouse embryogenesis. Using immunohistochemistry, we showed that MFG-E8 was ...
Source: exp Mol Med - July 25, 2015 Category: Molecular Biology Authors: Lee J, Choi BI, Park SY, An SY, Han J, Kim JH Tags: Exp Mol Med Source Type: research
Acute exposure to silica nanoparticles aggravate airway inflammation: different effects according to surface characteristics.
This study examined the effects of acute exposure to SNPs, either alone or in conjunction with ovalbumin (OVA), by studying the respiratory systems in exposed mouse models. Three types of SNPs were used: spherical SNPs (S-SNPs), mesoporous SNPs (M-SNPs), and PEGylated SNPs (P-SNPs). In the acute SNP exposure model performed, 6-week-old BALB/c female mice were intranasally inoculated with SNPs for 3 consecutive days. In the OVA/SNPs asthma model, the mice were sensitized two times via the peritoneal route with OVA. Additionally, the mice endured OVA with or without SNP challenges intranasally. Acute SNP exposure induced sig...
Source: exp Mol Med - July 20, 2015 Category: Molecular Biology Authors: Park HJ, Sohn JH, Kim YJ, Park YH, Han H, Park KH, Lee K, Choi H, Um K, Choi IH, Park JW, Lee JH Tags: Exp Mol Med Source Type: research
MicroRNA-103a-3p controls proliferation and osteogenic differentiation of human adipose tissue-derived stromal cells.
Abstract
The elucidation of the molecular mechanisms underlying the differentiation and proliferation of human adipose tissue-derived stromal cells (hADSCs) represents a critical step in the development of hADSCs-based cellular therapies. To examine the role of the microRNA-103a-3p (miR-103a-3p) in hADSCs functions, miR-103a-3p mimics were transfected into hADSCs in order to overexpress miR-103a-3p. Osteogenic differentiation was induced for 14 days in an osetogenic differentiation medium and assessed by using an Alizarin Red S stain. The regulation of the expression of CDK6 (cyclin-dependent kinase 6), a ...
Source: exp Mol Med - July 13, 2015 Category: Molecular Biology Authors: Sol Kim D, Young Lee S, Hee Lee J, Chan Bae Y, Sup Jung J Tags: Exp Mol Med Source Type: research
Honokiol ameliorates endothelial dysfunction through suppression of PTX3 expression, a key mediator of IKK/IκB/NF-κB, in atherosclerotic cell model.
Abstract
Pentraxin 3 (PTX3) was identified as a marker of the inflammatory response and overexpressed in various tissues and cells related to cardiovascular disease. Honokiol, an active component isolated from the Chinese medicinal herb Magnolia officinalis, was shown to have a variety of pharmacological activities. In the present study, we aimed to investigate the effects of honokiol on palmitic acid (PA)-induced dysfunction of human umbilical vein endothelial cells (HUVECs) and to elucidate potential regulatory mechanisms in this atherosclerotic cell model. Our results showed that PA significantly accele...
Source: exp Mol Med - July 4, 2015 Category: Molecular Biology Authors: Qiu L, Xu R, Wang S, Li S, Sheng H, Wu J, Qu Y Tags: Exp Mol Med Source Type: research
Emodin prevents intima thickness via Wnt4/Dvl-1/β-catenin signaling pathway mediated by miR-126 in balloon-injured carotid artery rats.
Abstract
Neointimal proliferation after vascular injury is a key mechanism of restenosis, a major cause of percutaneous transluminal angioplasty failure and artery bypass occlusion. Emodin, an anthraquinone with multiple physiological activities, has been reported to inhibit proliferation of vascular smooth muscle cells (VSMCs) that might cause intimal arterial thickening. Thus, in this study, we established a rat model of balloon-injured carotid artery and investigated the therapeutic effect of emodin and its underlying mechanism. Intimal thickness was analyzed by hematoxylin and eosin staining. Expressio...
Source: exp Mol Med - June 29, 2015 Category: Molecular Biology Authors: Hua JY, He YZ, Xu Y, Jiang XH, Ye W, Pan ZM Tags: Exp Mol Med Source Type: research
