Multiparameter cytometric analysis of complex cellular response
Cytometry Part A,Volume 93, Issue 2, Page 239-248, February 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - December 8, 2017 Category: Molecular Biology Source Type: research
---
Cytometry Part A,Volume 93, Issue 2, Page 239-248, February 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - December 8, 2017 Category: Molecular Biology Source Type: research
Analysis of NTPDase2 in the cell membrane using fluorescence recovery after photobleaching (FRAP)
Abstract
NTPDase2, a member of the CD39/NTPDase family, is an ecto‐nucleotidase anchored to the plasma membrane by two transmembrane domains, with a catalytic site facing the extracellular space and preferentially hydrolyzing nucleoside triphosphates. While NTPDase2 is expressed in many cell types, its unique functionality, mobility and dynamics at the cell membrane remain unexplored. We therefore constructed a recombinant NTPDase2 linked to the yellow fluorescent protein (EYFP) to investigate its dynamics by confocal microscopy. The present study shows that the expression of EYFP‐NTPDase2 in different cell lines does ...
Source: Cytometry Part A - December 1, 2017 Category: Molecular Biology Authors: Franciele Cristina Kipper, Alessandra Sayuri Kikuchi Tamajusuku, Darlan Conterno Minussi, Jos é Eduardo Vargas, Ana Maria Oliveira Battastini, Elzbieta Kaczmarek, Simon Christopher Robson, Guido Lenz, Márcia Rosângela Wink Tags: Brief Report Source Type: research
OMIP ‐044: 28‐color immunophenotyping of the human dendritic cell compartment
(Source: Cytometry Part A)
Source: Cytometry Part A - December 1, 2017 Category: Molecular Biology Authors: Florian Mair, Martin Prlic Tags: OMIP Source Type: research
Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study
Abstract
The BD FACSVia™ System features novel designs in hardware, software, and instrument QC. We compared the performance of the BD FACSVia System using the BD Leucocount™ kit with the BD FACSCalibur™ flow cytometer. Leucoreduced platelet (PLT, n = 252) and red blood cell (RBC, n = 278) specimens were enrolled at four sites. Each specimen was stained in four tubes using the BD Leucocount kit reagents and acquired on the two systems. BD Leucocount Control cells (high and low) were used to evaluate the inter‐site reproducibility on the BD FACSVia System at three sites over 20 days. Deming regression and Bland...
Source: Cytometry Part A - December 1, 2017 Category: Molecular Biology Authors: Yang Zeng, Michelle Dabay, Virginia George, Shalini Seetharaman, Monika de Arruda Indig, Sharon Graminske, Nicole Kimpel, Anna Schmidt, Amanda Boerner, Sarai Paradiso, Tatyana Delman, Yunyao Li, Viktoriya Litvak, Farzad Oreizy, Angela Chen, Maryam Salemin Tags: Original Article Source Type: research
