Volume 93A, Number 2, February 2018 Table of Contents
(Source: Cytometry Part A)
Source: Cytometry Part A - February 23, 2018 Category: Molecular Biology Tags: Online TOC Source Type: research
Issue Information – Editorial board
(Source: Cytometry Part A)
Source: Cytometry Part A - February 23, 2018 Category: Molecular Biology Tags: Issue Information – Editorial board Source Type: research
Volume 93A, Number 2, February 2018 Cover Image
Abstract
Microfluidics is all the rage in developing the simple‐structured, easily controlled and fast switched tools to diagnose diseases, test drugs and sort cells. The cover depicts a spark‐generated microbubble sell‐sorting process for microfluidic flow cytometry. Read the accompanying article by Zhao and You in the latest issue of Cytometry Part A
Cover design by Bärbel Beran [www.beran-design.de]. (Source: Cytometry Part A)
Source: Cytometry Part A - February 23, 2018 Category: Molecular Biology Tags: Volume 93A, Number 2, February 2018 Cover Image Source Type: research
Modeling of cytometry data in logarithmic space: When is a bimodal distribution not bimodal?
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - February 16, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - February 16, 2018 Category: Molecular Biology Source Type: research
Novel aspects of live intestinal epithelial cell function revealed using a custom time ‐lapse video microscopy apparatus
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - February 6, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - February 6, 2018 Category: Molecular Biology Source Type: research
Turning noise into order on the cell surface: Resonant activation of molecular highlighters
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Source Type: research
A guide to choosing fluorescent protein combinations for flow cytometric analysis based on spectral overlap
Abstract
The advent of facile genome engineering technologies has made the generation of knock‐in gene‐expression or fusion‐protein reporters more tractable. Fluorescent protein labeling of specific genes combined with surface marker profiling can more specifically identify a cell population. However, the question of which fluorescent proteins to utilize to generate reporter constructs is made difficult by the number of candidate proteins and the lack of updated experimental data on newer fluorescent proteins. Compounding this problem, most fluorescent proteins are designed and tested for use in microscopy. To addres...
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: Benjamin Kleeman, Andre Olsson, Tess Newkold, Matt Kofron, Monica DeLay, David Hildeman, H. Leighton Grimes Tags: Technical Note Source Type: research
A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation
This study aimed to characterize HSCs differentiation using a flow cytometry tool for fibrosis scoring. NK cells from healthy donors and from patients with chronic HCV with various severities of fibrosis were co‐cultured with a human HSC line (LX2). LX2 phagocytosis of NK cells were stained for NK cells (CD45/CD56/CD3) and NK activation marker (CD107a) as well as INF‐γ, apoptosis (Annexin‐V) and α‐smooth‐muscle‐actin (αSMA, as a marker of LX2 activation). In addition, reactive oxygen species (ROS) and the senescence marker P15 were analyzed prior to flow cytometry analysis. LX2 mono‐cultures demonstrated a...
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: Johnny Amer, Ahmad Salhab, Sarit Doron, Gilles Morali, Rifaat Safadi Tags: Original Article Source Type: research
A flow cytometry ‐based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against Chlamydia trachomatis
In this study, we, therefore, developed a simple and rapid flow cytometry based assay to measure the capacity of antibodies to mediate Fc‐receptor dependent phagocytosis. This method is highly reproducible and suitable to analyze large numbers of clinical and nonclinical samples. © 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of ISAC.
The illustration shows a simple, rapid, flow cytometry based assay to measure the capacity of antibodies to mediate Fc‐receptor dependent phagocytosis of labeled bacteria. (Source: Cytometry Part A)
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: Marco Grasse, Ida Rosenkrands, Anja Olsen, Frank Follmann, Jes Dietrich Tags: Original Article Source Type: research
Application of area scaling analysis to identify natural killer cell and monocyte involvement in the GranToxiLux antibody dependent cell ‐mediated cytotoxicity assay
Abstract
Several different assay methodologies have been described for the evaluation of HIV or SIV‐specific antibody‐dependent cell‐mediated cytotoxicity (ADCC). Commonly used assays measure ADCC by evaluating effector cell functions, or by detecting elimination of target cells. Signaling through Fc receptors, cellular activation, cytotoxic granule exocytosis, or accumulation of cytolytic and immune signaling factors have been used to evaluate ADCC at the level of the effector cells. Alternatively, assays that measure killing or loss of target cells provide a direct assessment of the specific killing activity of ant...
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: Justin Pollara, Chiara Orlandi, Charles Beck, R. Whitney Edwards, Yi Hu, Shuying Liu, Shixia Wang, Richard A. Koup, Thomas N. Denny, Shan Lu, Georgia D. Tomaras, Anthony DeVico, George K. Lewis, Guido Ferrari Tags: Original Article Source Type: research
Dynamic behavior of different quantities of osteoblasts during formation of micromass cultures
This study aims to investigate and clarify the extent to which cell quantity influences the dynamics of micromass formation of osteoblastic cell cultures. For this purpose, the migration and aggregation during this process are investigated by optical inspection employing image processing software that allows for automated tracking of cell groups using digital image correlation. An exponential time behavior is observed with respect to the velocity of the cells and the distance of the cells to their common center of gravity. Characteristic time constants are derived as quantitative measures of the cell dynamics. The results ...
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: Susanne Sch äfer, Kent Urban, Maria Gerber, Markus Dekiff, Dieter Dirksen, Ulrich Plate Tags: Brief Report Source Type: research
Cellular endogenous NAD(P)H fluorescence as a label ‐free method for the identification of erythrocytes and reticulocytes
Abstract
Reticulocytes and erythrocytes are the ultimate differentiated stages of erythropoiesis. In addition to being anucleate cells, they are characterized by the clearance of their mitochondrial pool or lack thereof. Given that for most research‐oriented flow cytometry experiments erythrocytes and reticulocytes are often undesirable cell types, their identification and exclusion from analyses can be essential. Here, we describe a flow cytometric method based on cellular NAD(P)H‐related autofluorescence, whose localization is mainly associated with mitochondria. By increasing the sensitivity of the specific NAD(P)H...
Source: Cytometry Part A - February 1, 2018 Category: Molecular Biology Authors: L. Tauzin, V. Campos, M. Tichet Tags: Technical Note Source Type: research
