A field ‐applicable method for flow cytometric analysis of granulocyte activation: Cryopreservation of fixed granulocytes
Abstract
Upon activation granulocytes upregulate several adhesion molecules (CD11b) and granule proteins (CD35, CD66b) and shed surface l‐selectin (CD62L). These changes in expression, as assessed by flow cytometry, can be used as markers for activation. Whereas these markers are usually studied in fresh blood samples, a new method is required when samples are collected at a field site with no direct access to a flow cytometer. Therefore, we developed and tested a field‐applicable method in which fixed leukocytes were cryopreserved. Using this method, the intensity of granulocyte activation markers was compared to samp...
Source: Cytometry Part A - March 13, 2018 Category: Molecular Biology Authors: Karin de Ruiter, Selma van Staveren, Bart Hilvering, Edward Knol, Nienke Vrisekoop, Leo Koenderman, Maria Yazdanbakhsh Tags: Original Article Source Type: research
Model ‐free quantification and visualization of colocalization in fluorescence images
Abstract
The spatial association between fluorescently tagged biomolecules in situ provides valuable insight into their biological relationship. Within the limits of diffraction, such association can be measured using either Pearson's Correlation Coefficient (PCC) or Spearman's Rank Coefficient (SRC), which are designed to measure linear and monotonic correlations, respectively. However, the relationship between real biological signals is often more complex than these measures assume, rendering their results difficult to interpret. Here, we have adapted methods from the field of information theory to measure the associatio...
Source: Cytometry Part A - March 13, 2018 Category: Molecular Biology Authors: Aaron B. Taylor, Maria S. Ioannou, Jesse Aaron, Teng ‐Leong Chew Tags: Original Article Source Type: research
OMIP ‐046: Characterization of invariant T cell subset activation in humans
(Source: Cytometry Part A)
Source: Cytometry Part A - March 13, 2018 Category: Molecular Biology Authors: Kerri G. Lal, Edwin Leeansyah, Johan K. Sandberg, Michael A. Eller Tags: OMIP Source Type: research
A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - March 8, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - March 8, 2018 Category: Molecular Biology Source Type: research
A flow cytometry ‐based assay to determine the phagocytic activity of both clinical and nonclinical antibody samples against Chlamydia trachomatis
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - March 7, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - March 7, 2018 Category: Molecular Biology Source Type: research
Application of area scaling analysis to identify natural killer cell and monocyte involvement in the GranToxiLux antibody dependent cell ‐mediated cytotoxicity assay
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - March 2, 2018 Category: Molecular Biology Source Type: research
Antibody ‐based cell‐surface proteome profiling of metastatic breast cancer primary explants and cell lines
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - March 2, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - March 2, 2018 Category: Molecular Biology Source Type: research
Antibody ‐based cell‐surface proteome profiling of metastatic breast cancer primary explants and cell lines
Abstract
Flow cytometric cell surface proteomics provides a new and powerful tool to determine changes accompanying neoplastic transformation and invasion, providing clues to essential interactions with the microenvironment as well as leads for potential therapeutic targets. One of the most important advantages of flow cytometric cell surface proteomics is that it can be performed on living cells that can be sorted for further characterization and functional studies. Here, we document the surface proteome of clonogenic metastatic breast cancer (MBrCa) explants, which was strikingly similar to that of normal mesenchymal str...
Source: Cytometry Part A - March 2, 2018 Category: Molecular Biology Authors: Vera S. Donnenberg, Jayce Jieming Zhang, Erika Moravcikova, Ernest Michael Meyer, Haihui Lu, Christian T. Carson, Albert D. Donnenberg Tags: Original Article Source Type: research
Dynamic behavior of different quantities of osteoblasts during formation of micromass cultures
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - March 1, 2018 Category: Molecular Biology Source Type: research
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Cytometry Part A, Ahead of Print. (Source: Cytometry Part A)
Source: Cytometry Part A - March 1, 2018 Category: Molecular Biology Source Type: research
Cellular endogenous NAD(P)H fluorescence as a label ‐free method for the identification of erythrocytes and reticulocytes
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - February 26, 2018 Category: Molecular Biology Source Type: research
