Concurrent detection of lysosome and tissue transglutaminase activation in relation to cell cycle position during apoptosis induced by different anticancer drugs
AbstractDescribed is the new cytometric approach do detect either stimulation or a collapse of lysosomal proton pump (lysosomes rupture) combined with activation of transglutaminase 2 (TG2) during induction of apoptosis. Apoptosis of human lymphoblastoid TK6 cells was induced by combination of 2 ‐deoxyglucose with the isoquinoline alkaloid berberine, by DNA topoisomerase I inhibitor camptothecin, its analog topotecan, topoisomerase II inhibitors etoposide or mitoxantrone, as well as by the cytotoxic anticancer ribonuclease ranpirnase (onconase). Activity of the proton pump of lysosomes wa s assessed by measuring entrapme...
Source: Cytometry Part A - November 13, 2018 Category: Molecular Biology Authors: H. Dorota Halicka,
Jiangwei Li,
Hong Zhao,
Zbigniew Darzynkiewicz Tags: Original Article Source Type: research
Next ‐generation amplicon TRB locus sequencing can overcome limitations of flow‐cytometric Vβ expression analysis and confirms clonality in all T‐cell prolymphocytic leukemia cases
AbstractT ‐cell receptor (TCR) β repertoire analysis can distinguish monoclonal from polyclonal T‐cell proliferations and crucially aid in the diagnosis of T‐cell malignancies. TCR repertoire can be assessed either by flow cytometry (FCM), or by molecular genetic techniques. We compared the results of parallel analyses of Vβ expression by FCM andTRB rearrangements by DNA ‐based next‐generation sequencing (NGS) in 80 diagnostic peripheral blood samples of patients with T‐cell prolymphocytic leukemia (T‐PLL) for (1) the diagnosis of clonality and (2) the assessment of dominant Vβ usage. FCM‐based analysis ...
Source: Cytometry Part A - November 10, 2018 Category: Molecular Biology Authors: Michaela Kotrova,
Michaela Novakova,
Sebastian Oberbeck,
Petra Mayer,
Alexandra Schrader,
Henrik Knecht,
Ondrej Hrusak,
Marco Herling,
Monika Br üggemann Tags: Original Article Source Type: research
Could the key be hidden in the lysosomes?
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - November 1, 2018 Category: Molecular Biology Authors: Gloria Juan Source Type: research
CD40 signaling —germinal center b cells go at their own pace
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - November 1, 2018 Category: Molecular Biology Authors: Thomas Liechti Source Type: research
A comparative study of two separation methods to isolate monocytes
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - October 31, 2018 Category: Molecular Biology Authors: Ronald Weiss,
Wilhelm Gerdes,
Franziska Leonhardt,
Rommy Berthold,
Ulrich Sack,
Anja Grahnert Source Type: research
Effect of storage time and temperature on cell cycle analysis by mass cytometry
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - October 31, 2018 Category: Molecular Biology Authors: Raymond D. Devine,
Palak Sekhri,
Gregory K. Behbehani Source Type: research
Minimum degree of overlap between IL ‐9R and IL‐2R on human T lymphoma cells: A quantitative CLSM and FRET analysis
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - October 31, 2018 Category: Molecular Biology Authors: Enik ő Nizsalóczki,
Péter Nagy,
Gábor Mocsár,
Ágnes Szabó,
István Csomós,
Thomas A. Waldmann,
György Vámosi,
László Mátyus,
Andrea Bodnár Source Type: research
OMIP ‐050: A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint Molecules
Cytometry Part A, EarlyView. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Authors: Leonard Nettey,
Amber J. Giles,
Pratip K. Chattopadhyay Source Type: research
Cytometry part A instructions to contributors
Cytometry Part A,Volume 93, Issue 10, Page 1072-1072, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
ISAC member application
Cytometry Part A,Volume 93, Issue 10, Page 1067-1068, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
Editorial Policy
Cytometry Part A,Volume 93, Issue 10, Page 1066-1066, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
Journal roundup
Cytometry Part A,Volume 93, Issue 10, Page 975-975, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
In this issue
Cytometry Part A,Volume 93, Issue 10, Page 974-974, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
Volume 93A, Number 10, October 2018 Cover Image
Cytometry Part A,Volume 93, Issue 10, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
Issue Information – Publication schedule
Cytometry Part A,Volume 93, Issue 10, Page 971-971, October 2018. (Source: Cytometry Part A)
Source: Cytometry Part A - October 22, 2018 Category: Molecular Biology Source Type: research
