Chemical design and bioefficacy screening of new insect growth regulators as potential insecticidal agents against Spodoptera littoralis (Boisd.)
Publication date: Available online 12 November 2019Source: Biotechnology ReportsAuthor(s): Antar A. Abdelhamid, M.M. Elwassimy, Safwat A. Aref, Mohamed A. GadAbstractThe 13 new compounds were chemically synthesized and their spectroscopic analysis was done to determine their chemical structure. All the compounds were screened for their insecticidal potential against Spodoptera littoralis (Boisd.). Among the tested compounds, the compound 13 was found to be the most potent. It displayed one fold more activity than a reported insect growth regulator, fenoxycarb. The other target compounds demonstrated weak to strong toxicolo...
Source: Biotechnology Reports - November 13, 2019 Category: Biotechnology Source Type: research
Genome-Wide Identification of Doublesex and Mab-3-Related Transcription Factor (DMRT) Genes in Nile Tilapia (Oreochromis niloticus)
Publication date: Available online 11 November 2019Source: Biotechnology ReportsAuthor(s): Imran zafar, Mohd Ashraf Rather, Bhushan C. DhandareAbstractDoublesex and Mab-3-related transcription factor (DMRT) gene family is extensively known for its contribution in sex determination and differentiation across phyla. Here we report the identification of five DM (doublesex and mab-3) domain genes in the Nile tilapia which includes DMRT1, DMRTa2, DMRT2a, DMRT2b and DMRT3a. The full-length sequence of DMRT genes ranges from 3526 (DMRTA2) to 1471bp (DMRT1) which encode putative proteins series from 469 to 372 amino acids. All the...
Source: Biotechnology Reports - November 12, 2019 Category: Biotechnology Source Type: research
Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines
Publication date: Available online 9 November 2019Source: Biotechnology ReportsAuthor(s): Sadegh Khorrami, Atefeh Zarepour, Ali ZarrabiAbstract"Black Peel Pomegranate" is a rare pomegranate cultivar that its specific features are still uncovered particularly in the bio-nano researches. The present study was organized to evaluate this pomegranate's potential in the biosynthesis of silver nanoparticles as well as bio-medical activities. According to the results, the pomegranate peel extract incredibly inhibited 100% of DPPH free radicals (EC50 = 5 μg/mL). This extract also induced more than 70% cell death in the treat...
Source: Biotechnology Reports - November 10, 2019 Category: Biotechnology Source Type: research
Biochemical similarity between cultured chondrocytes and in situ chondrocytes by chemometric analysis from FTIR microspectroscopy
ConclusionThe biochemical composition and biodistribution analysis using FTIRM have been useful for comparing cultured chondrocytes and in situ chondrocytes. (Source: Biotechnology Reports)
Source: Biotechnology Reports - November 10, 2019 Category: Biotechnology Source Type: research
Protective responses of an engineered PspA recombinant antigen against Streptococcus pneumoniae
In this study, we developed two immunogenic antigens based on recombinant PspA proteins that including the different antigenic regions of PspA from both two families. The cross-reactivity of antibodies elicited against two PspA proteins PspAB1-5 and PspA4ABC and their role in complement deposition with three strains of pneumococci were tested. The protective effects of developed anti-PspA antibodies in mice in intranasal and intraperitoneal challenges were evaluated using a strain from clade 2. Sera from immunized mice with PspAB1-5 in comparison with PspA4ABC was able to deposit more C3 complement component on surface of ...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research
Biodegradation of phenanthrene as a model hydrocarbon: power display of a super-hydrophobic halotolerant enriched culture derived from a saline-sodic soil
In this study, after evaluating the degradation activity of enriched cultures from four crude oil-contaminated soils in mineral salt medium, the most efficient ones were selected for further studies. The chemical analysis of cell-free extract containing phenanthrene by HPLC suggested the superior enriched culture was able to degrade 87.66% of phenanthrene at the concentration of 40 mg L-1 within 10 days. This experiment was done under optimal conditions (37 °C, 10% salinity, and pH around 7 to 7.5). The 16S rRNA sequencing of isolates from this superior enriched culture indicated the highest similarity to Acidovorax...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research
Heterologous expression and functional characterization of a GH10 endoxylanase from Aspergillus fumigatus var. niveus with potential biotechnological application
Publication date: Available online 30 October 2019Source: Biotechnology ReportsAuthor(s): Josman Velasco, Bianca Oliva, Evandro José Mulinari, Leidy Patricia Quintero, Awana da Silva Lima, Aline Larissa Gonçalves, Thiago Augusto Gonçalves, André Damasio, Fabio Marcio Squina, Adriane Maria Ferreira Milagres, Asmaa Abdella, Mark R. Wilkins, Fernando SegatoAbstractXylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var. niveus (AFUMN-GH10) was successfully e...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research
RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance
This study reports on the ability of a transgene-derived RNA hairpin, homologous to an overlapping region of the SACMV replication associated protein and putative virus suppressor of silencing protein (AC1/AC4), to confer tolerance in the CMD-susceptible model cassava cultivar 60444. Three of the fourteen transgenic lines expressing SACMV AC1/AC4 hairpin-derived siRNAs showed decreased symptoms and viral loads compared to untransformed control plants. Expression of SACMV AC1/AC4 homologous siRNAs showed that this tolerance is most likely associated with post-transcriptional gene silencing of the virus. This is the first re...
Source: Biotechnology Reports - November 1, 2019 Category: Biotechnology Source Type: research
In vitro proliferation and differentiation of canine bone marrow derived mesenchymal stem cells over hydroxyl functionalized CNT substrates
Publication date: Available online 27 October 2019Source: Biotechnology ReportsAuthor(s): A.P. Madhusoodan, Kinsuk Das, Bhabesh Mili, Kuldeep Kumar, Ajay Kumar, A.C. Saxena, Praveen Singh, Triveni Dutt, Sadhan BagAbstractNanotopography of culture substrate acts as a positive cue in cell-biomaterial based tissue regeneration. Considering the potentiality of carbon nanotubes (CNTs) this study was designed to evaluate its two functionalized form by an in vitro culture condition using canine mesenchymal stem cells as cellular model. Cells were isolated and its behaviour, proliferation and differentiation processes were elucida...
Source: Biotechnology Reports - October 29, 2019 Category: Biotechnology Source Type: research
Nematicidal Activity of Thermostable Alkaline Protease Produced by Saccharomonospora viridis strain Hw G550
Publication date: Available online 25 October 2019Source: Biotechnology ReportsAuthor(s): Osama M. Darwesh, Ahmad S. El-Hawary, Usama S. El Kelany, Gamal M. El-SherbinyAbstractApplication of thermostable alkaline protease to control the harmful nematodes was investigated in the current study. A total of 14 proteolytic actinomycetes were isolated from Egyptian harsh environments. Out of them, isolate G550 exhibited the highest proteolytic activity (528.9 U/ml). Protease from isolate G550 exhibited high nematicidal activity against M. incognita under laboratory conditions and caused hydrolysis of J2S cuticle. This isolate wa...
Source: Biotechnology Reports - October 26, 2019 Category: Biotechnology Source Type: research
Enzymatic hydrolysis of Opuntia ficus-indica cladode by Acinetobacter pittii and alcohol fermentation by Kluyveromyces marxianus: pH, temperature and microorganism effect
Publication date: Available online 25 October 2019Source: Biotechnology ReportsAuthor(s): C.M. López-Domínguez, M.O. Ramírez-Sucre, I.M. Rodríguez-BuenfilAbstractIn the present work the effect of three factors: pH, temperature and type of microorganism using a factorial design 32x2 was evaluated on: growth, total cellulases hydrolytic activity (FPases), endoglucanases hydrolytic activity (CMCases), free reducing sugars (FRS), glucose, sucrose, and alcohol production using a culture medium based on mineral salts added with cladodes flour of Opuntia ficus-indica at 20% as the unique carbon source. Two wild microorganisms...
Source: Biotechnology Reports - October 26, 2019 Category: Biotechnology Source Type: research
In silico analysis of functional linkage among arsenic induced MATE genes in rice
Publication date: Available online 24 October 2019Source: Biotechnology ReportsAuthor(s): Snigdhamayee Seth, Sandip Debnath, NR ChakrabortyAbstractMATE genes play an important role in cellular detoxification processes. Nine MATE genes were identified by a transcriptomics study previously. Candidate gene prioritization was done where 29 new genes were found to interact with 09 guide genes. Therefore, a total of 38 genes were analyzed here to predict a concise model by gene prioritization study. Those genes were analyzed further in Rice Interactions Viewer programme, and based on high ICV, 10 new genes were found to interact...
Source: Biotechnology Reports - October 25, 2019 Category: Biotechnology Source Type: research
Mutagenicity, genotoxicity and oxidative stress induced by pesticide industry wastewater using bacterial and plant bioassays
Publication date: Available online 24 October 2019Source: Biotechnology ReportsAuthor(s): Mohammad Tarique Zeyad, Kumar Murugan, Abdul MalikAbstractAtomic absorption spectrophotometer and gas chromatography analysis revealed the presence of heavy metals, organochlorine and organophosphate pesticides in industrial wastewater. XAD, Dichloromethane and n-Hexane extracted wastewater were analysed for genotoxic potential using Ames Salmonella/mammalian microsome test. The XAD concentrated sample displayed remarkable mutagenic activity compared to solvent assisted liquid–liquid extraction. Strain TA98 was found utmost sensitiv...
Source: Biotechnology Reports - October 25, 2019 Category: Biotechnology Source Type: research
Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides)
Publication date: Available online 5 October 2019Source: Biotechnology ReportsAuthor(s): Pavel A. Levashov, Darya A. Matolygina, Oxana A. Dmitrieva, Ekaterina D. Ovchinnikova, Irina Yu. Adamova, Nataliya V. Karelina, Vladimir A. Nelyub, Nikolay L. Eremeev, Andrey V. LevashovAbstractChemical modification of lysozyme was carried out using benzaldehyde and anisaldehyde. It was shown that chemical modification affects only 1-2 amino groups of the protein molecule which does not prevent further covalent immobilization of lysozyme using the remaining free amino groups. The bacteriolytic activity of lysozyme is preserved after ch...
Source: Biotechnology Reports - October 5, 2019 Category: Biotechnology Source Type: research
Red light controlled callus morphogenetic patterns and secondary metabolites production in Withania somnifera L
This study concludes that red light treatment was optimum for maximum biomass accumulation and anti-oxidant activity in calli of W. somnifera. (Source: Biotechnology Reports)
Source: Biotechnology Reports - September 20, 2019 Category: Biotechnology Source Type: research
