Plant growth-promoting activities and genomic analysis of the stress-resistant Bacillus megaterium STB1, a bacterium of agricultural and biotechnological interest
Publication date: Available online 4 December 2019Source: Biotechnology ReportsAuthor(s): Francisco X. Nascimento, Anabel G. Hernández, Bernard R. Glick, Márcio J. RossiAbstractIn this work, the stress-resistant Bacillus megaterium STB1 is characterized and its ability to promote plant growth under normal and stress conditions is demonstrated. The genomic sequence of this bacterium, and a detailed analysis of the genes involved in facilitating its stress resistance and plant growth-promoting activities is also reported.The B. megaterium STB1 genome is rich in genetic elements involved in multiple stress resistance, xenob...
Source: Biotechnology Reports - December 5, 2019 Category: Biotechnology Source Type: research
Optimization of Carotenoids production by Rhodotorula mucilaginosa (MTCC-1403) using agro-industrial waste in Bioreactor: A statistical approach
Publication date: Available online 5 December 2019Source: Biotechnology ReportsAuthor(s): Rajan Sharma, Gargi GhoshalAbstractBio-colorants are preferred over synthetic colors as bio-colorants not only impart characteristic color to the food also contain harmless bio-active antioxidant nutrients. The present study was undertaken to investigate the potential of agro-industrial waste (Onion peels, potato skin, mung bean husk and pea pods) for carotenoid production from Rhodotorula mucilaginosa. After screening of appropriate carbon, nitrogen sources from agro-industrial waste, the fermentation conditions (pH, temperature, agi...
Source: Biotechnology Reports - December 5, 2019 Category: Biotechnology Source Type: research
Study of antioxidant activity and some herbal compounds of Dracocephalum kotschyi Boiss. in different ages of growth
ConclusionBut in general, it can be said that the six-year-old plant with the highest antioxidant capacity, phenolic, and flavonoid compounds is the best age and mainly flower organ is preferred. (Source: Biotechnology Reports)
Source: Biotechnology Reports - December 4, 2019 Category: Biotechnology Source Type: research
Sustainability potentials of novel laccase tinctures from Stenotrophomonas maltophilia BIJ16 and Bordetella bronchiseptica HSO16: from dye decolorization to denim bioscouring
Publication date: Available online 3 December 2019Source: Biotechnology ReportsAuthor(s): John O. UnuofinAbstractThe aim of this present study was to investigate the environmental proficiency of two laccase producing bacterial strains, Hb16c and Berl11b2. Here, laccase which were secreted in media containing environmental wastes were characterized for biochemical and kinetic novelty and applied in the decolourization of some synthetic dyes and subsequently, denim bleaching. The laccases exhibited enhanced pH-, thermo-, psychro-, metal-, halo-, and surfacto-tolerance, eliciting residual activities of at least ca. 71%. There...
Source: Biotechnology Reports - December 4, 2019 Category: Biotechnology Source Type: research
Controlling Anthracnose By Means of extracts, and their major constituents, from Brosimum rubescens Taub
Publication date: Available online 30 November 2019Source: Biotechnology ReportsAuthor(s): Janio Martinez, Andrés Gomez, Cesar Ramirez, Jesús Gil, Diego DurangoAbstractAnthracnose, caused by the fungus Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., is the most limiting fungal disease of mango and papaya crops in Colombia. The in vivo and in vitro activity against C. gloeosporioides of the extracts from sawdust of Brosimum rubescens Taub. (Moraceae) was evaluated. The extracts of less polarity (n-hexane and dichloromethane) displayed the greatest inhibitory effects. Then, the coumarins xanthyletin (2.74% d.w.) and ...
Source: Biotechnology Reports - November 30, 2019 Category: Biotechnology Source Type: research
Selective and sensitive colorimetric detection of platinum using Pseudomonas stutzeri mediated optimally synthesized antibacterial silver nanoparticles
Publication date: Available online 22 November 2019Source: Biotechnology ReportsAuthor(s): Megha P. Desai, Reshma V. Patil, Kiran D. PawarAbstractIn this work, Pseudomonas stutzeri was used for the optimum biogenic synthesis of antibacterial silver nanoparticles (AgNPs) which were applied for colorimetric detection of platinum ions (Pt+2). The optimum synthesis conditions were 2 mM AgNO3, pH 9 and incubation at 60 °C for 24 h. The FTIR spectra indicated that biomolecules such as amino acids, proteins or enzymes from P. stutzeri were involved in the synthesis of AgNPs in the size range of 10-50 nm. Among the variou...
Source: Biotechnology Reports - November 23, 2019 Category: Biotechnology Source Type: research
Draft genomes and initial characteriaztion of siderophore producing pseudomonads isolated from mine dump and mine drainage
Publication date: Available online 23 November 2019Source: Biotechnology ReportsAuthor(s): Marika Hofmann, Thomas Heine, Vivian Schulz, Sarah Hofmann, Dirk TischlerAbstractSiderophores are of high interest for biotechnological, pharmaceutical, agricultural and industrial applications. Although they are synthesized by various organisms, the yield is usually low which hindrances their suitability for broad range uses. Thus, it is necessary to identify novel producers and to increase the understanding of the biosynthesis pathways. Herein we report the isolation of two novel Pseudomonas strains and the identification of the ge...
Source: Biotechnology Reports - November 23, 2019 Category: Biotechnology Source Type: research
Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
Publication date: Available online 21 November 2019Source: Biotechnology ReportsAuthor(s): Mitsuki Sasaki, Akari Takagi, Shuhei Ota, Shigeyuki Kawano, Daisaku Sasaki, Munehiko AsayamaAbstractA novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG110 medium (BG11—N) with 2% CO2-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivat...
Source: Biotechnology Reports - November 22, 2019 Category: Biotechnology Source Type: research
Biogas production from recycled paper mill wastewater by UASB digester: optimal and mesophilic conditions
In this study, the AD experiment was performed in continuous mode with an effluent inlet flow rate equal to 1 L/h. Using the optimal OLR value 5.18 g COD/Ld and with an effluent 5,7 g COD/L, a biogas yield of 92 N mL/g COD removed (at normal temperature and pressure) was obtained during the RPMW anaerobic treatment. The reactor was operated at an optimal hydraulic retention time (HRT) of 15.14 h with a biogas production volume of the optimal value 62.5 L/d. These results indicate that RPMW can be effectively treated in a UASB reactor with the advantage of producing biogas. We tested our system with RPMW, to s...
Source: Biotechnology Reports - November 22, 2019 Category: Biotechnology Source Type: research
Household aluminum foil matte and bright side reflectivity measurements: Application to a photobioreactor light concentrator design
Publication date: Available online 20 November 2019Source: Biotechnology ReportsAuthor(s): Victor Pozzobon, Wendie Levasseur, Khanh-Van Do, Bruno Palpant, Patrick PerréAbstractThis work reports the design of a light concentrator intended to be used to cast uniform lighting over a photobioreactor. Household aluminum foils was chosen as reflective material to build the concentrator. This choice raised the question of which side to use. Thus measurements of household aluminum foil reflectivity spectra on both bright and matte sides were undergone. These measurements were done using an integrating sphere, over a 250–2500&nb...
Source: Biotechnology Reports - November 21, 2019 Category: Biotechnology Source Type: research
Monitoring method for transgene expression in target tissue by blood sampling
In this study, we have developed a novel method to monitor transgene expression in tissues by blood sampling. We administered plasmid DNA (pDNA) encoding non-secretory form of firefly luciferase as a reporter gene and pDNA encoding secretable Gaussia princeps luciferase as a monitor gene simultaneously into mice. Good positive correlations were found between log-transgene expression of the reporter gene and the monitor gene in the treated muscle, between the monitor gene in the treated muscle and plasma, and consequently between the reporter gene in the treated muscle and the monitor gene in plasma after naked pDNA transfe...
Source: Biotechnology Reports - November 16, 2019 Category: Biotechnology Source Type: research
Hyper-production of Taxol from Aspergillus fumigatus, an endophytic fungus isolated from Taxus sp. of the Northern Himalayan region
Publication date: Available online 15 November 2019Source: Biotechnology ReportsAuthor(s): Pradeep Kumar, Balwant Singh, Vikram Thakur, Abhishek Thakur, Nandita Thakur, Deepak Pandey, Duni ChandAbstractTaxol® (generic name Paclitaxel) is a chemotherapeutic drug, effective against head, neck, breast, lung, bladder, ovary, and cervix cancers. Rising demands in chemotherapy and limited supply of natural taxol have ultimately increased the cost of the drug. Semi synthesis using taxol precursors is not able to meet the global supply and has intensified the need to find alternative ways of taxol production. In the present study...
Source: Biotechnology Reports - November 16, 2019 Category: Biotechnology Source Type: research
A simple, efficient and rapid screening technique for differentiating nitrile hydratase and nitrilase producing bacteria
Publication date: Available online 15 November 2019Source: Biotechnology ReportsAuthor(s): Ruchi Sahu, Anil Kumar Meghavarnam, Savitha JanakiramanAbstractNitrile hydrolyzing enzymes catalyze the hydration of nitrile compounds to corresponding amides and acids. Bacteria, isolated from soil samples were screened for nitrile hydrolyzing enzymes by simple dye based 96 well plate and nesslerization method. Bromothymol blue was used as an indicator for the detection of amides and acids based on colour change of the indicator dye from blue to dark green or yellow. The screening assay also differentiates between nitrile hydratase ...
Source: Biotechnology Reports - November 15, 2019 Category: Biotechnology Source Type: research
The current strategies and parameters for the enhanced microbial production of 2,3-butanediol
Publication date: Available online 13 November 2019Source: Biotechnology ReportsAuthor(s): Olivier Hakizimana, Emmanuel Matabaro, Byong H. LeeAbstract2,3-Butanediol (2,3-BD) is a propitious compound with many industrial uses ranging from rubber, fuels, and cosmetics to food additives. Its microbial production has especially attracted as an alternative way to the petroleum-based production. However, 2,3-BD production has always been hampered by low yields and high production costs. The enhanced production of 2,3-butanediol requires screening of the best strains and a systematic optimization of fermentation conditions. Moreo...
Source: Biotechnology Reports - November 15, 2019 Category: Biotechnology Source Type: research
Maltose deterioration approach: Catalytic behavior optimization and stability profile of maltase from Bacillus licheniformis KIBGE-IB4
In this study, the catalytic behavior of maltase was optimized under various physicochemical condition. Results indicated that bacterial maltase exhibited maximum catalytic activity at 45 °C and pH-6.5 after 5.0 min. It presented greater stability within 0.1 M K2HPO4 buffer having pH-6.5 and showed 100% activity even after 1.0 h. It retained 83.6% and 45.0% activity at 40 °C after 1.0 and 3.0 h, respectively. The enzyme retained 90.0% activity at ˗20 °C even after 60 days. The molecular weight of enzyme was deduced to be 157.2 kDa as calculated using polyacrylamide gel electrophoresis (PAGE) and zymogra...
Source: Biotechnology Reports - November 13, 2019 Category: Biotechnology Source Type: research
