Integrated control of dry root rot of chickpea caused by Rhizoctoniabataticola under the natural field condition
Publication date: March 2020Source: Biotechnology Reports, Volume 25Author(s): Abdul Khaliq, Sartaj Alam, Irfan Ullah Khan, Dilawar Khan, Shakela Naz, Yaxin Zhang, Assar Ali ShahAbstractThe study investigated the effect of three fungicides Bavistin®, Aliette®, and Biomagic® at the rate of 2 g/L were tested In vitro and In vivo to control dry root rot and their impact on yield were evaluated at the rate of 4 g/L against R. bataticola while varieties used were Karak-1, Karak-2, Karak-3, and Sheenghar. Disease severity was recorded lowest (19 %) and highest yield (1467 Kg/ha), plant height (47.5 cm), number of pods/plant (...
Source: Biotechnology Reports - January 23, 2020 Category: Biotechnology Source Type: research
Integrated control of dry root rot of chickpea caused by Rhizoctonia Bataticola under the natural field condition
Publication date: Available online 20 January 2020Source: Biotechnology ReportsAuthor(s): Abdul Khaliq, Sartaj Alam, Irfan Ullah Khan, Dilawar Khan, Shakela Naz, Yaxin Zhang, Assar Ali ShahAbstractThe study investigated the effect of three fungicides Bavistin®, Aliette®, and Biomagic® at the rate of 2 g/L were tested In vitro and In vivo to control dry root rot and their impact on yield were evaluated at the rate of 4 g/L against R. bataticola while varieties used were Karak-1, Karak-2, Karak-3, and Sheenghar. Disease severity was recorded lowest (19%) and highest yield (1467 Kg/ha), plant height (47.5 cm), number...
Source: Biotechnology Reports - January 21, 2020 Category: Biotechnology Source Type: research
Immobilisation of Lipase enzyme onto bacterial magnetosomes for stain removal
Publication date: Available online 17 January 2020Source: Biotechnology ReportsAuthor(s): Jobin John Jacob, Suthindhiran KAbstractLipase was immobilized onto bacterial magnetosomes using glutaraldehyde cross-linking and confirmed by Fourier transform infrared spectrometry (FT-IR) and Scanning electron microscopy (SEM). Enzyme activity of immobilised lipase as well as free lipase was estimated by the release of p-nitro phenol due to the hydrolysis of p-nitro phenyl acetate (pNPA). The immobilisation yield of lipase onto magnetosome was found to be 88%. The optimal pH (7) and temperature (40 °C) for activity was standardi...
Source: Biotechnology Reports - January 17, 2020 Category: Biotechnology Source Type: research
Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher
Publication date: Available online 11 January 2020Source: Biotechnology ReportsAuthor(s): Hiroki Akiba, Kensuke Takayanagi, Osamu Kusano-Arai, Hiroko Iwanari, Takao Hamakubo, Kouhei TsumotoAbstractBiparatopic fragment antibodies can overcome deficiencies in avidity of conventional antibody fragments. Here, we describe a technology for generating biparatopic antibodies through two-step targeting using a pair of polypeptides, SpyTag and SpyCatcher, that spontaneously react to form a covalent bond between antibody fragments. In this method, two antibody fragments, each targeting different epitopes of the antigen, are fused to...
Source: Biotechnology Reports - January 12, 2020 Category: Biotechnology Source Type: research
The complete genome sequence of the algicidal bacterium Bacillus subtilis strain JA and the use of quorum sensing to evaluate its antialgal ability
We describe the isolation of Bacillus subtilis strain JA and demonstrate that this bacterium exhibited strong algicidal effects on the algae Alexandrium minutum with an inhibition rate exceeding 80% within 48 h. B. subtilis JA significantly reduced the photosynthetic efficiency of A. minutum and caused extensive morphological damage to the algae. Genomic analysis of B. subtilis JA demonstrated that a putative AI-2 type quorum sensing (QS) gene (LuxS) is present in its genome cluster, which is regulate pheromone biosynthesis. Interestingly, the exogenous addition of a QS-oligopeptide (ComX-pheromone) improved the algicidal ...
Source: Biotechnology Reports - January 11, 2020 Category: Biotechnology Source Type: research
Phosphorus Removal from Landfill Leachate by Microalgae
Publication date: Available online 7 January 2020Source: Biotechnology ReportsAuthor(s): Zareen T. KhanzadaAbstractPhosphorus is an essential constituent of all living organisms but it is non-renewable and its natural reserves are fast depleting. Phosphorus discharged in wastewater could be sustainably reused by microalgae. Knowledge about cellular phosphorus dynamics in microalgae has been rapidly advancing and luxury phosphorus (poly-P) uptake phenomenon by microalgae is becoming the focus point for many research studies. Ultra-membrane treated landfill leachate was used as a nutrient medium for the growth of indigenous ...
Source: Biotechnology Reports - January 8, 2020 Category: Biotechnology Source Type: research
Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers in Morus alba L. cv. Chinese white
Publication date: Available online 3 January 2020Source: Biotechnology ReportsAuthor(s): Gulab Khan Rohela, Phanikanth Jogam, Mohammad Yaseen Mir, Aftab Ahmad Shabnam, Pawan Shukla, Sadanandam Abbagani, Azra Nahaid KamiliAbstractA reliable protocol was developed for in vitro micro propagation of Morus alba L.cv. Chinese white. Initially, friable callus was induced (242.8 and 128.5 mg) from in vivo leaf and nodal explants on Murashige and Skoog’s (MS) medium amended with 4.0 µM/L of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 3.0 µM/L of Naphthalene acetic acid (NAA) respectively within 3 weeks. Shoot regeneration (12....
Source: Biotechnology Reports - January 5, 2020 Category: Biotechnology Source Type: research
Impact of process parameters and plant polysaccharide hydrolysates in cellulase production by Trichoderma reesei and Neurospora crassa under wheat bran based solid state fermentation
Publication date: Available online 2 January 2020Source: Biotechnology ReportsAuthor(s): Nitin Verma, Vivek KumarAbstractLignocellulosic biomass is a promising feedstock for future renewable fuels. It constitutes a substantial renewable substrate for the production of value-added chemicals. Lignocellulosic materials mostly contain a mixture of polymers such as cellulose, hemicelluloses, and lignin. Utilizing waste lignocellulosic materials such as agricultural residues, grasses, forestry wastes can significantly reduce the cost of raw materials. Optimization of significant process parameters is also a very important stage ...
Source: Biotechnology Reports - January 3, 2020 Category: Biotechnology Source Type: research
Draft genome sequence and nomenclature adjustment of Rhodococcus qingshengii CS98, a cesium-accumulating strain isolated in Japan
Publication date: Available online 31 December 2019Source: Biotechnology ReportsAuthor(s): Candice Raeburn, Shin Kasahara, Toshi Komoda, Catherine Abbott, Peter M SmookerAbstractStrains within the Rhodococcus genus have the ability to endure a range of recalcitrant compounds and metabolise a variety of pollutants. As a result there is increasing interest in these robust prokaryotes for their applications in bioremediation of contaminated environments and bioconversion of industrial wastes. In this announcement we present the draft genome sequence of R. qingshengii CS98, a soil isolate from Japan with the demonstrated abili...
Source: Biotechnology Reports - January 2, 2020 Category: Biotechnology Source Type: research
Investigation of antifouling properties of polypropylene/TiO2 nanocomposite membrane under different aeration rate in membrane bioreactor system
Publication date: Available online 31 December 2019Source: Biotechnology ReportsAuthor(s): Habib Etemadi, Milad Fonouni, Reza YeganiAbstractThis paper presents the results of aeration rate effect on antifouling properties of polypropylene (PP)/TiO2 nanocomposite membrane in membrane bioreactor (MBR) system in order to oil refinery wastewater treatment. For this purpose, three levels of aeration rate with specific aeration demand per membrane area (SADm) of 0.5, 1, and 1.5 m3/m2h was used. According to the obtained results, PP nanocomposite membrane showed high hydrophilicity, porosity, and high flux compared to neat PP m...
Source: Biotechnology Reports - January 2, 2020 Category: Biotechnology Source Type: research
Relevance of biogas technology to vhembe district of the limpopo province in south africa
In this study, the sampled households involved 72 biogas users and 128 non-users. The sampling techniques was purposive and simple random. The study was based on primary data that was elicited using open and closed-ended questionnaires. The logistic regression model was employed for data analysis. The energy demand of low-income South African households for cooking with fuelwood was found to be 27 MJ/day and the total energy demand 68 MJ/day. Approximately 625 000 households in Vhembe district can potentially benefit from bio-digester fed with cattle and pig waste, on the basis of livestock numbers. Most governments wo...
Source: Biotechnology Reports - December 21, 2019 Category: Biotechnology Source Type: research
Draft genomes and initial characterization of siderophore producing pseudomonads isolated from mine dump and mine drainage
Publication date: March 2020Source: Biotechnology Reports, Volume 25Author(s): Marika Hofmann, Thomas Heine, Vivian Schulz, Sarah Hofmann, Dirk TischlerAbstractSiderophores are of high interest for biotechnological, pharmaceutical, agricultural and industrial applications. Although they are synthesized by various organisms, the yield is usually low which hindrances their suitability for broad range uses. Thus, it is necessary to identify novel producers and to increase the understanding of the biosynthesis pathways. Herein we report the isolation of two novel Pseudomonas strains and the identification of the gene clusters ...
Source: Biotechnology Reports - December 18, 2019 Category: Biotechnology Source Type: research
Isolation and characterization of an alkali and thermostable laccase from a novel Alcaligenes faecalis and its application in decolorization of synthetic dyes
Publication date: Available online 14 December 2019Source: Biotechnology ReportsAuthor(s): Seema Mehandia, S.C. Sharma, Shailendra Kumar AryaAbstractA laccase producing new bacterial strain (Alcaligenes faecalis XF1) was isolated from green site of Chandigarh (India) by standard screening method. Nutrient broth medium containing 0.2 mM CuSO4 was used for the production of laccase. Maximum production (110 U/ml) was achieved after four days of incubation. The extracellular laccase from the medium was purified by simple salt precipitation and ion exchange technique to get 3.8 fold purified protein with 1637.8 U/mg specific ...
Source: Biotechnology Reports - December 15, 2019 Category: Biotechnology Source Type: research
Sequential degradation of raw vinasse by a laccase enzyme producing fungus Pleurotus sajor-caju and its ATPS purification
This study evaluated simultaneously the raw vinasse degradation, an effluent from the sugar-alcohol industry, the laccase production by Pleurotus sajor-caju and its purification using aqueous two-phase systems (ATPS). To improve laccase production, different concentrations of inducers (ethanol and CuSO4) were added. The higher laccase production promoted an increase of 4-fold using 0.4 mM of CuSO4 as inducer, with maximum enzymatic activity of 539.3 U/L on the 3rd day of fermentation. The final treated vinasse had a decolorization of 92% and turbidity removal of 99% using CuSO4. Moreover, the produced laccase was then pu...
Source: Biotechnology Reports - December 13, 2019 Category: Biotechnology Source Type: research
Removal of Pb (II) from aqueous solution using nanoadsorbent of Oryza sativa husk: Isotherm, Kinetic and Thermodynamic studies
Publication date: Available online 6 December 2019Source: Biotechnology ReportsAuthor(s): Mandeep Kaur, Santosh Kumari, Praveen SharmaAbstractThis research focus on the removal of Pb (II) ions from aqueous solution by adsorption process using nanoadsorbent developed from agricultural waste Oryza sativa husk (OSH). Surface morphology of nanoadsorbent was analyzed by FE-SEM, elemental composition by EDX and size by AFM. Attachment of functional groups on nanoadsorbent was determined by FTIR. The effect of pH, dose, contact time, initial concentration and temperature were investigated. Optimum adsorption of lead at pH 8, cont...
Source: Biotechnology Reports - December 7, 2019 Category: Biotechnology Source Type: research
