Composition and functional profiling of the microbiota in the casts of Eisenia fetida during vermicomposting of brewers’ spent grains
Publication date: Available online 20 February 2020Source: Biotechnology ReportsAuthor(s): Marilena Budroni, Ilaria Mannazzu, Severino Zara, Sara Saba, Antonio Pais, Giacomo ZaraAbstractVermicomposting is a cost-effective biotechnology for the management of organic wastes that relies on the activity of earthworms and their associated microbiota. Here, the microbiotas of the earthworm Eisenia fetida fed with brewers’ spent grains (FBSG), cow manure (FCM) and a mix of brewers’ spent grains/cow manure (FMIX), were identified by high-throughput DNA sequencing (16S rRNA). Bacterial community variance correlated with the pH ...
Source: Biotechnology Reports - February 21, 2020 Category: Biotechnology Source Type: research
Molecular weight and viscosifying power of alginates produced by mutant strains of Azotobacter vinelandii under microaerophilic conditions
Publication date: Available online 20 February 2020Source: Biotechnology ReportsAuthor(s): Andres García, Tania Castillo, Diego Ramos, Carlos L. Ahumada-Manuel, Cinthia Núñez, Enrique Galindo, Jochen Büchs, Carlos PeñaAbstractAlginates are polysaccharides that are of interest in various industrial applications. This is due to the viscosifying properties of alginates, which depends on the weight-average molecular weight. The aim of the present study was to evaluate the changes in alginate quality, in terms of the viscosifying power and weight-average molecular weight of the polymer produced by Azotobacter vinelandii mu...
Source: Biotechnology Reports - February 21, 2020 Category: Biotechnology Source Type: research
Identification of a Novel 1,2 Oxazine That Can Induce Apoptosis by Targeting NF-κB in Hepatocellular Carcinoma Cells
Publication date: Available online 19 February 2020Source: Biotechnology ReportsAuthor(s): Chaithanya Somu, Chakrabhavi Dhananjaya Mohan, Sachin Ambedkar, Dukanya, Shobith Rangappa, CP Baburajeev, Alexey Sukhorukov, Srishti Mishra, Muthu K Shanmugam, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Sulaiman Ali Alharbi, Basappa, Kanchugarakoppal S. RangappaAbstractConstitutive activation of NF-κB is associated with proinflammatory diseases and suppression of the NF-κB signaling pathway has been considered as an effective therapeutic strategy in the treatment of various cancers including hepatocellular carcinoma (HCC). Her...
Source: Biotechnology Reports - February 20, 2020 Category: Biotechnology Source Type: research
Bio-selective hormonal breast cancer cytotoxic and antioxidant potencies of Melia azedarach L. wild type leaves: Running title: M. azedarach bio-selective anti breast cancer potency
Publication date: Available online 17 February 2020Source: Biotechnology ReportsAuthor(s): Martha Ervina, Hadi Poerwono, Retno Widyowati, Katsuyoshi Matsunami, SukardimanAbstractMelia azedarach L. is used widely in traditional medicine for local or systemic ailments. Although studies exist on phytochemicals and potencies of Chinese and Indian cultivars of Melia, the present study investigated in vitro antioxidant properties of Melia wild type and its cytotoxicity against T47D cell. The ethanolic extract of the Melia leaves was fractionated with n-hexane, ethyl acetate and water, and the secondary metabolites were obtained....
Source: Biotechnology Reports - February 18, 2020 Category: Biotechnology Source Type: research
Mesenchymal stem cells proliferation and remote manipulation upon exposure to magnetic semiconductor nanoparticles
Publication date: Available online 11 February 2020Source: Biotechnology ReportsAuthor(s): Tudor Braniste, Vitalie Cobzac, Polina Ababii, Irina Plesco, Simion Raevschi, Alexandru Didencu, Mihail Maniuc, Viorel Nacu, Ion Ababii, Ion TiginyanuAbstractIn this paper, we report on spatial redistribution of bone marrow mesenchymal stem cells loaded with magnetic nanoparticles under the influence of continuously applied magnetic field. Semiconductor nanoparticles were synthesized by epitaxial growth of a GaN thin layer on magnetic sacrificial core consisting of ZnFe2O4 nanoparticles. Different quantities of nanoparticles were inc...
Source: Biotechnology Reports - February 12, 2020 Category: Biotechnology Source Type: research
Optimization of recombinant Zika virus NS1 protein secretion from HEK293 cells
Publication date: Available online 11 February 2020Source: Biotechnology ReportsAuthor(s): Julieta S. Roldán, Alejandro Cassola, Daniela S. CastilloAbstractSensitive, accurate and cost-effective diagnostic tests are urgently needed to detect Zika virus (ZIKV) infection. Nonstructural 1 (NS1) glycoprotein is an excellent diagnostic marker since it is released in a hexameric conformation from infected cells into the patient's bloodstream early in the course of the infection. We established a stable rZNS1-His-expression system in HEK293 cells through lentiviral transduction. A novel optimization approach to enhance rZNS1-His...
Source: Biotechnology Reports - February 12, 2020 Category: Biotechnology Source Type: research
Characterization of Soluble Algal Products (SAPs) After Electrocoagulation of a Mixed Algal Culture
Publication date: Available online 10 February 2020Source: Biotechnology ReportsAuthor(s): Poorya Rafiee, Sirous Ebrahimi, Maryam Hosseini, Yen Wah TongAbstractThe dewatering of algal culture requires coagulation of the algal cells. However, the coagulation in a continuous operation is slowed down through the excretion of Soluble Algal Products (SAPs). Electrocoagulation (EC), already utilized as a coagulation technique, has been investigated for its effects on SAPs characterizations. A mixed culture of Chlorella vulgaris, Scenedesmus Obliquus, Botryococcus braunii, Botryococcus sudeticus, and Afrocarpus falcatus was prepa...
Source: Biotechnology Reports - February 11, 2020 Category: Biotechnology Source Type: research
Viability of microencapsulated and non-microencapsulated Lactobacilli in a commercial beverage
In conclusion, this study suggests that the encapsulation and double coating of L. acidophilus and L. rhamnosus can increase the viability of them in Doogh beverage and in simulated GI conditions. (Source: Biotechnology Reports)
Source: Biotechnology Reports - February 6, 2020 Category: Biotechnology Source Type: research
Manganese (II) removal from aqueous solutions by Cladosporium halotolerans and Hypocrea jecorina
This article reported the Mn removal of two fungi strains isolated from mine wastewater. ITS rRNA region sequencing identified the fungi strains as Cladosporium halotolerans and Hypocrea jecorina. Mn2+ removal assays were performed in Sabouraud broth with 50 mg L-1 Mn2+ supplemented and bioleaching assays using MnO2 instead of MnSO4 at the same conditions. C. halotolerans removed 96% of 50 mg L-1 Mn2+ at two weeks without MnO2 bioleaching with 649.9 mg of biomass and H. jecorina removed about 50% of Mn2+ in 21 days from initial 50 mg of Mn2+ L-1 with 316.8 mg of biomass. Extracellular laccases were pres...
Source: Biotechnology Reports - February 5, 2020 Category: Biotechnology Source Type: research
Metabolomic applications for understanding complex tripartite plant-microbes interactions: Strategies and Perspectives
Publication date: Available online 1 February 2020Source: Biotechnology ReportsAuthor(s): Adetomiwa Ayodele Adeniji, Olubukola Oluranti Babalola, Du Toit LootsAbstractPhytopathogens from the Alternaria sp., Fusarium sp., Penicillium sp., and Pseudomonas sp. and their toxigenic metabolites - alternariol, fumonisin, citrinin, and coronatine respectively, negatively impact crop yields and sales by eliciting plant diseases and/or causing human and veterinary toxicoses upon the consumption of contaminated food. These phytopathogens and their associated toxins, however, are present and most likely in undetectable concentrations ...
Source: Biotechnology Reports - February 2, 2020 Category: Biotechnology Source Type: research
Microalgae polysaccharides bio-stimulating effect on tomato plants: growth and metabolic distribution
Publication date: Available online 31 January 2020Source: Biotechnology ReportsAuthor(s): Farid Rachidi, Redouane Benhima, Laila Sbabou, Hicham El ArroussiAbstractMicroalgae polysaccharides represent a potentially bioressource for the enhancement and the protection of agricultural crops. We investigate the possibility to use microalgae polysaccharides as a plant biostimulant. The crude polysaccharides extract (PS) from three microalgae strains were applied to Solanum lycopersicum plants by irrigation and compared basing on their effects on shoot and root length, nodes number and shoot and root dry weight. The application o...
Source: Biotechnology Reports - February 1, 2020 Category: Biotechnology Source Type: research
Green synthesis of metallic nanoparticles as effective alternatives to treat antibiotics resistant bacterial infections: A review
Publication date: Available online 31 January 2020Source: Biotechnology ReportsAuthor(s): Anirudh Singh, Pavan Kumar Gautam, Arushi Verma, Vishal Singh, P. M. Shiva Priya, Saurabh Shivalkar, Amaresh Kumar Sahoo, Sintu Kumar SamantaAbstractDue to development of bacterial resistance to the conventional antibiotics, the treatment of bacterial infections has become a major issue of concern. The unprescribed and uncontrolled use of antibiotics has lead to the rapid development of antibiotic resistance in bacterial strains. Therefore, the development of novel and potent bactericidal agents is of great clinical importance. Intere...
Source: Biotechnology Reports - February 1, 2020 Category: Biotechnology Source Type: research
Porous starch citrate biopolymer for controlled release of carbofuran in the management of root knot nematode meloidogyne incognita
Publication date: Available online 30 January 2020Source: Biotechnology ReportsAuthor(s): Oluwatoyin FabiyiAbstractThe undesirable environmental impacts of inappropriate application of pesticides have brought about research into new matrices for controlled release of pesticides. Porous starch citrate biopolymer was designed for the release of carbofuran in this experiment and characterized using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermo-Gravimetric Analysis (TGA) for functional group, surface morphology and thermal stability properties respectively. The SEM revealed highl...
Source: Biotechnology Reports - February 1, 2020 Category: Biotechnology Source Type: research
2-Phenylethanol and 2-phenylethylacetate production by nonconventional yeasts using tequila vinasses as a substrate
In this study, four yeasts species (Wickerhamomyces anomalus, Candida glabrata, Candida utilis, and Candida parapsilosis) were evaluated with two different chemically defined media and tequila vinasses. Differences in the aroma compounds production were observed depending on the medium and yeast species used. In tequila vinasses, the highest concentration (65 mg/L) of 2-PEA was reached by C. glabrata, the inhibitory compounds decreased biomass production and synthesis of 2-PEA, and biochemical and chemical oxygen demands were reduced by more than 50%. Tequila vinasses were suitable for the production of 2-phenylethylacet...
Source: Biotechnology Reports - January 26, 2020 Category: Biotechnology Source Type: research
At-line high throughput site-specific glycan profiling using targeted mass spectrometry
Publication date: Available online 22 January 2020Source: Biotechnology ReportsAuthor(s): Bertie Chi, Christel Veyssier, Toyin Kasali, Faisal Uddin, Christopher A. SellickAbstractProtein post-translational modification (PTM) plays an important role in many biological processes; of which glycosylation is arguably one of the most complex and diverse modifications and is crucial for the safety and efficacy of biotherapeutic proteins.Mass spectrometric characterization of protein glycosylation is well established with clear advantages and disadvantages; on one hand it is precise and information-rich, as well as being relative ...
Source: Biotechnology Reports - January 23, 2020 Category: Biotechnology Source Type: research
