Development of a highly sensitive PCR/DNA chip method to detect mycoplasmas in a veterinary modified live vaccine
Publication date: July 2018Source: Biologicals, Volume 54Author(s): Sylvie Mbelo, Virginie Gay, Stephanie Blanchard, Eric Abachin, Stephanie Falque, Jacques Lechenet, Hervé Poulet, Blandine de Saint-VisAbstractMycoplasmas are potential contaminants that introduce undesirable changes in mammalian cell cultures. They frequently contaminate cell substrates and other starting materials used for manufacturing cell-derived biologics, such as vaccines and pharmaceutical products. Mycoplasma purity testing of live vaccines, active ingredients, raw material, and seed lots is required during vaccine production. Previously, testing ...
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Adherence to biologic therapy – Does it vary with ethnicity?
ConclusionTo our knowledge, this is the first study to demonstrate ethnic differences in disease activity score and related negative beliefs regarding subcutaneous biologic therapies in people with rheumatic diseases. (Source: Biologicals)
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Electrospun composite PLLA/Oyster shell scaffold enhances proliferation and osteogenic differentiation of stem cells
Publication date: July 2018Source: Biologicals, Volume 54Author(s): Roghaieh Didekhani, Mahmoud Reza Sohrabi, Ehsan Seyedjafari, Masoud Soleimani, Hana Hanaee-AhvazAbstractIn bone tissue engineering, bioceramics are of the most widely used materials for treatment of bone defects clinically. The composites of bioceramic/polymer fibrous scaffolds have been designed and developed to fulfill the mechanical and biological requirements of the damaged tissue. In the present study, oyster shell (OS) as a bioceramic in combination with the biodegradable and biocompatible poly (l-lactide) has been used to prepare a new tissue-engine...
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Coculture of conjunctiva derived mesenchymal stem cells (CJMSCs) and corneal epithelial cells to reconstruct the corneal epithelium
This study aimed to find an improved and convenient system for the corneal epithelial differentiation of conjunctiva derived mesenchymal stem cells (CJMSCs). Thus, the cells were used to reconstruct corneal epithelial cells. Obtained by flow cytometry data, 51.9% of isolated CJMSCs were immune reactive for SSEA4+ antibody which are more potent to differentiate into corneal epithelial cells. A differential medium in a single culture plate was applied and compared to a Coculture with a SHEM medium and a Coculture with a commercial medium. It was found that CJMSCs can be induced to corneal epithelial cells through in vitro co...
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Development of an anti-EPO antibody detection kit based on lab-on-a-chip and bridging antibody technologies
Publication date: July 2018Source: Biologicals, Volume 54Author(s): Jin-Gyo Oh, Jihyun Seong, Sunmi Han, Tae-Hwe HeoAbstractImmunogenicity is a major concern in the use of biological drugs. In particular, antibody-mediated pure red cell aplasia (PRCA) is a rare condition that is caused by administration of recombinant erythropoietin. There are numerous assay platforms for detect EPO anti-drug antibody (ADA), and most have appropriate assay sensitivity, but in need of improvement in terms of assay turnaround time and user accessibility. Here, the new method was developed based on lab-on-a-chip technology and bridging ELISA....
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Evaluation of toll-like receptor 4 expression in human bone marrow mesenchymal stem cells by lipopolysaccharides from Shigella
In this study, we investigate the effects of various concentrations of LPS from different Shigella strains on TLR4 expression in human bone marrow (hBM)-MSCs. At the mRNA level, we have found that untreated hBM-MSCs (control) did not express TLR4 compared to the experimental groups. Cells treated with LPS from Shigella flexneri had the highest expression of TLR4, whereas cells treated with LPS from Shigella sonnei had the lowest expression. We observed that LPSs had a dose-dependent effect on TLR4 expression in all of the treatment groups. ELISA findings for interleukin-6 secretion have confirmed mRNA expression results fo...
Source: Biologicals - July 11, 2018 Category: Biology Source Type: research
Distribution and absence of generalized lesions in mice following single dose intramuscular inoculation of the vaccine candidate MVA-MERS-S
Publication date: July 2018Source: Biologicals, Volume 54Author(s): Martin C. Langenmayer, Anna-Theresa Lülf-Averhoff, Silvia Adam-Neumair, Robert Fux, Gerd Sutter, Asisa VolzAbstractModified Vaccinia Virus Ankara (MVA) is a highly attenuated and replication-deficient virus serving as vaccine against infectious diseases. Here, we assessed the in vivo distribution of a recombinant MVA candidate vaccine against the Middle Eastern Respiratory Syndrome (MVA-MERS-S) in mice. Intramuscularly inoculated mice were necropsied at different time points and examined by histology, immunohistochemistry and real-time PCR. We detected in...
Source: Biologicals - July 6, 2018 Category: Biology Source Type: research
Aims and Scope/Editorial Board/Publishing Details
Publication date: July 2018Source: Biologicals, Volume 54Author(s): (Source: Biologicals)
Source: Biologicals - July 6, 2018 Category: Biology Source Type: research
Coculture of conjunctiva derived mesenchymal stem cells (CJMSCs) and corneal epithelial cells to reconstruct the corneal epithelium
This study aimed to find an improved and convenient system for the corneal epithelial differentiation of conjunctiva derived mesenchymal stem cells (CJMSCs). Thus, the cells were used to reconstruct corneal epithelial cells. Obtained by flow cytometry data, 51.9% of isolated CJMSCs were immune reactive for SSEA4+ antibody which are more potent to differentiate into corneal epithelial cells. A differential medium in a single culture plate was applied and compared to a Coculture with a SHEM medium and a Coculture with a commercial medium. It was found that CJMSCs can be induced to corneal epithelial cells through in vitro co...
Source: Biologicals - July 6, 2018 Category: Biology Source Type: research
Animal cell substrates for the production of biologicals: An introduction to WHO documents
Publication date: Available online 9 December 2014Source: BiologicalsAuthor(s): John Petricciani, Ivana Knezevic (Source: Biologicals)
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
Therapeutic monoclonal antibody N-glycosylation – Structure, function and therapeutic potential
Publication date: March 2018Source: Biologicals, Volume 52Author(s): Florian Cymer, Hermann Beck, Adelheid Rohde, Dietmar ReuschAbstractTherapeutic antibodies (IgG-type) contain several post-translational modifications (PTMs) whereby introducing a large heterogeneity, both structural and functional, into this class of therapeutics. Of these modifications, glycosylation in the fragment crystallizable (Fc) region is the most heterogeneous PTM, which can affect the stability of the molecule and interactions with Fc-receptors in vivo. Hence, the glycoform distribution can affect the mode of action and have implications for bio...
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
Evaluation of the use of various rat strains for immunogenic potency tests of Sabin-derived inactivated polio vaccines
Publication date: March 2018Source: Biologicals, Volume 52Author(s): Yuichi Someya, Yasushi Ami, Reiko Takai-Todaka, Akira Fujimoto, Kei Haga, Kosuke Murakami, Yoshiki Fujii, Haruko Shirato, Tomoichiro Oka, Takashi Shimoike, Kazuhiko Katayama, Takaji WakitaAbstractSlc:Wistar rats have been the only strain used in Japan for purpose of evaluating a national reference vaccine for the Sabin-derived inactivated polio vaccine (sIPV) and the immunogenicity of sIPV-containing products. However, following the discovery that the Slc:Wistar strain was genetically related to the Fischer 344 strain, other “real” Wistar strains, suc...
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
Sex bias in mouse humoral immune response to influenza vaccine depends on the vaccine type
Publication date: March 2018Source: Biologicals, Volume 52Author(s): Irena Živković, Raisa Petrović, Nevena Arsenović-Ranin, Vladimir Petrušić, Rajna Minić, Biljana Bufan, Olga Popović, Gordana LeposavićAbstractThe study explored influence of biological sex on development of humoral immune response to seasonal trivalent whole inactivated virus (WIV) and split virus (SV) influenza vaccines in outbred Swiss mouse model. To this end, mice of both sexes were immunized with WIV (WIV mice) and SV vaccines (SV mice) and examined for specific antibody response. Irrespective of sex, total IgG and neutralizing antibody resp...
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
Evaluation of cellular immunogenicity of recombinant cytochrome p450 cyp141 protein of Mycobacterium tuberculosis in human and mouse model
Publication date: March 2018Source: Biologicals, Volume 52Author(s): Mahshid salehi, Rahman Abdizadeh, Batoul Pourgheysari, Behnam Zamanzad, Amin Soltani, Abolfazl Gholipour, Reza HeidariAbstractMycobacterium tuberculosis (Mtb) is still considered one of the unsolved problems for the World Health Organization Identifying and selecting an immunogenic antigen capable of generating specific immune responses is generally the goal of all studies being carried out in to designing new vaccines. Accordingly, the present study was conducted to evaluate the immunogenicity of a M. tuberculosis recombinant protein which exist in the r...
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFβ3)
Conclusion, our data implies that miR-140 is a potent chondrogenic differentiation inducer for iPSCs and also, we have showed increasing chondrogenic differentiation by using overexpression of miR-140 and TGFβ3. (Source: Biologicals)
Source: Biologicals - July 5, 2018 Category: Biology Source Type: research
