The evaluation of an immunoperoxidase assay applicable in antiviral drug screening
In this study, the application of an immunoperoxidase assay in the screening of antiviral drugs was tested using two known BVDV inhibitors, ribavirin and ammonium chloride (NH4Cl). Phospholipase C inhibitor U73122 was identified to affect BVDV infection by using this immunoperoxidase assay. In addition, the results of immunoperoxidase assay were validated by real-time PCR. Taken together, the immunoperoxidase assay is a useful and versatile method suitable for antiviral drug screening targeting NCP-BVDV. (Source: Biologicals)
Source: Biologicals - November 24, 2018 Category: Biology Source Type: research
Development and validation of a robust multiplex serological assay to quantify antibodies specific to pertussis antigens
Publication date: Available online 18 November 2018Source: BiologicalsAuthor(s): Gowrisankar Rajam, George Carlone, Ellie Kim, Jin Choi, Simon Paulos, SoHee Park, Amilia Jeyachandran, Yamini Gorantla, Emily Wong, Amit Sabnis, Peter Browning, Rita Desai, Conrad P. Quinn, Jarad SchifferAbstractDespite wide spread vaccination, the public health burden of pertussis remains substantial. Current acellular pertussis vaccines comprise upto five Bordetella pertussis (Bp) antigens. Performing an ELISA to quantify antibody for each antigen is laborious and challenging to apply to pediatric samples where serum volume may be limited. W...
Source: Biologicals - November 18, 2018 Category: Biology Source Type: research
Avian leukosis virus subgroup – J as a contaminant in live commercially available poultry vaccines distributed in Nigeria
Publication date: Available online 16 November 2018Source: BiologicalsAuthor(s): Ismaila Shittu, Adeyinka J. Adedeji, Pam D. Luka, Olayinka O. Asala, Nancy M. Sati, Ijeoma O. Nwagbo, Chinonyerem N. Chinyere, Oluwadamilola O. Arowolo, Jolly A. Adole, Paulinus Emennaa, Paul A. Abdu, Tony M. JoannisAbstractGlobally, vaccines are used to prevent and control the menace of infectious diseases in livestock with some reported to be inadvertently contaminated with extraneous agents (EAs). With the aim of screening and characterizing for some selected EAs, 44 live viral poultry vaccines were randomly selected based on availability. ...
Source: Biologicals - November 17, 2018 Category: Biology Source Type: research
Determination of residual poly diallyldimethylammonium chloride (pDADMAC) in monoclonal antibody formulations by size exclusion chromatography and evaporative light scattering detector
Publication date: Available online 14 November 2018Source: BiologicalsAuthor(s): Mehdi Khodadadian, Maryam Ghassemi, Hossein Behrouz, Shayan Maleknia, Fereidoun MahboudiAbstractThe cationic polyelectrolyte pDADMAC is widely used in biopharmaceutical industry as a flocculating agent to enhance clarification throughput and downstream filtration operations. Due to the possible toxicity, pDADMAC should be assessed for an acceptable residual level to ascertain the safety of the product to patients. The strong protein-polyelectrolyte interaction, however, can negatively affect sensitivity and accuracy of measurements. This paper...
Source: Biologicals - November 15, 2018 Category: Biology Source Type: research
Aims and Scope/Editorial Board/Publishing Details
Publication date: November 2018Source: Biologicals, Volume 56Author(s): (Source: Biologicals)
Source: Biologicals - November 11, 2018 Category: Biology Source Type: research
Differentiation of stem cells from the apical papilla into osteoblasts by the elastic modulus of porous silk fibroin scaffolds
Publication date: Available online 2 November 2018Source: BiologicalsAuthor(s): Mehdi Amirikia, Seyed Gholam Ali Jorsaraei, Seyed Mohammad Ali Shariatzadeh, Malek Soleimani MehranjaniAbstractIn addition to traditional approach for differentiation (biochemical factors), physical properties (elastic modulus) of the matrix will be becoming an important factor in differentiation of stem cells into different lineages. The porous scaffolds were prepared from the silk fibroin solutions 4%, 5%, 6%, 7% and 8.4% weight per volume (w/v) by freeze drying which produced scaffolds with the minimal changes in the pore size (96–160 μ...
Source: Biologicals - November 3, 2018 Category: Biology Source Type: research
Human single-chain variable fragment antibody expressed in E. coli with optimal in vitro cross-neutralizing and no enhancing activity
Publication date: Available online 26 October 2018Source: BiologicalsAuthor(s): Surachet Benjathummarak, Chonlatip Pipattanaboon, Khwanchit Boonha, Waranya Wongwit, Pongrama Ramasoota, Pannamthip PitaksajjakulAbstractSingle chain fragment variable (scFv) is a small molecule antibody comprising of only the variable region of heavy and light chain responsible for antigen binding. For dengue disease, the Fc region of antibody molecule was reported to be involved with dengue complication caused by Antibody-dependent enhancement (ADE). We attempted to produce small molecule scFv human monoclonal antibody (HuMAb), which lacking ...
Source: Biologicals - October 27, 2018 Category: Biology Source Type: research
Optimized upstream and downstream process conditions for the improved production of recombinant human asparaginase (rhASP) from Escherichia coli and its characterization
Publication date: Available online 13 October 2018Source: BiologicalsAuthor(s): Rajesh Kumar Kante, Sandeep Vemula, Silpa Somavarapu, Maheswara Reddy Mallu, B.H. Boje Gowd, Srinivasa Reddy RondaAbstractThe present work elucidates the production of recombinant human asparaginase (rhASP) under optimized fermentation and downstream processes in Escherichia coli. The maximum biomass yield of 6.7 g/L was achieved with fed-batch fermentation. The highest rhASP inclusion bodies recovery yield (91%) was achieved with the optimized lysis conditions. The 8.0 M urea at pH 8.5 has shown efficient solubilization (94%) of rhASP incl...
Source: Biologicals - October 13, 2018 Category: Biology Source Type: research
The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells
This study shows that estrogen affects the expression of specific markers of primordial germ cells. Also, estrogen and retinoic acid speed up and increase the level of expression of specific markers. (Source: Biologicals)
Source: Biologicals - October 9, 2018 Category: Biology Source Type: research
Aims and Scope/Editorial Board/Publishing Details
Publication date: September 2018Source: Biologicals, Volume 55Author(s): (Source: Biologicals)
Source: Biologicals - September 8, 2018 Category: Biology Source Type: research
Establishment of the first International Standard for human anti-typhoid capsular Vi polysaccharide IgG
Publication date: Available online 7 September 2018Source: BiologicalsAuthor(s): Sjoerd Rijpkema, Jason Hockley, Alastair Logan, Peter Rigsby, Eleanor Atkinson, Celina Jin, David Goldblatt, Haoyu Liang, Novilia S. Bachtiar, Jae Seung Yang, Akshay Goel, Venkatesan Ramasamy, Marcela F. Pasetti, Andrew J. Pollard, the anti-Vi IgG working groupAbstractVi capsular polysaccharide (Vi) conjugate vaccines, which can prevent typhoid in infants and young children, are being developed. Comparative immunogenicity studies are facilitated by an International Standard (IS) for human anti-Vi IgG. 16/138, a pool of sera from volunteers whi...
Source: Biologicals - September 8, 2018 Category: Biology Source Type: research
Immunization with Salmonella Abortusequi phage lysate protects guinea pig against the virulent challenge of SAE-742
Publication date: Available online 1 September 2018Source: BiologicalsAuthor(s): Abhishek, B. Kumar, Anjay, A.K. Mishra, C. Prakash, A. Priyadarshini, M. RawatAbstractSalmonella Abortusequi causes important clinical diseases in horses possibly leading to abortion. In the present investigation, the protective efficacy of both plain and aluminum hydroxide gel adjuvanted phage lysate was evaluated in guinea pig model. Broad host range bacteriophage PIZ-SAE-2, was characterized and used for generation of lysates. Three different lysate batches, produced through separate cycles and characterized, were pooled together for immuni...
Source: Biologicals - September 2, 2018 Category: Biology Source Type: research
Influence of hydrodynamic pressure on chondrogenic differentiation of human bone marrow mesenchymal stem cells cultured in perfusion system
Publication date: Available online 31 August 2018Source: BiologicalsAuthor(s): Soheila Zamanlui, Leila Mohammadi Amirabad, Masoud Soleimani, Shahab FaghihiAbstractThe natural conditions of chondrocytes in native cartilage including mechanical forces and surface topology could be simulated to enhance chondrogenesis. A perfusion system recapitulating the hydrodynamic pressure of cartilage tissue is designed. Mesenchymal stem cells (MSCs) are isolated and seeded on aligned nanofibrous PCL/PLGA scaffolds that mimic the structure of superficial zone of articular cartilage. The cell-seeded scaffolds are placed into the perfusion...
Source: Biologicals - September 1, 2018 Category: Biology Source Type: research
A risk assessment on extraneous virus contamination in a viral vaccine production environment justifies waiving of in vivo testing
Publication date: Available online 31 August 2018Source: BiologicalsAuthor(s): Uffe B. Westergaard, Bodil van Overeem Hansen, Kasper Abildgaard Rud, Claus Nielsen, Gitte Stawski, Kaare R. HasløvAbstractFor many years in vivo assays have been a corner stone in safety testing of vaccines for human use. However, there is now an increasing regulatory focus on replacement, reduction and refinement of methods involving animal use. Accordingly, European Pharmacopoeia (Ph.Eur.) monographs and chapters are currently being revised to reduce or discontinue the use of animals in safety and other testing, when such in vivo tests are n...
Source: Biologicals - August 31, 2018 Category: Biology Source Type: research
Preparation of F(ab’)2 antivenom in Iraq against scorpion (Hottentotta saulcyi) venom
Publication date: Available online 25 August 2018Source: BiologicalsAuthor(s): Brwa Nawzad Ismael, Kasim Sakran Abass, Khalil Abdulla Khalil, Kamal Ali SalihAbstractThe aim of this study was to prepare specific F(ab’)2 antivenom against Iraqi scorpion (Hottentotta saulcyi). Venom was obtained by electrical stimulation method, the scorpions venom storage capacity was 1.7 ± 0.4 mg and LD50 found to be 1.07 mg/kg by subcutaneous (s.c) route. Three local horses aging 3–4 years were selected for immunization. During the schedule each horse received 0.5–14 mg venom in 49 days by s.c route. The horses immune resp...
Source: Biologicals - August 26, 2018 Category: Biology Source Type: research
