Evaluation of carboxyl beads based latex agglutination test for rapid sero-diagnosis of Japanese encephalitis
Publication date: Available online 10 September 2019Source: BiologicalsAuthor(s): M.R. Grace, Jyotsana Chauhan, M. Suman Kumar, Ashok Kumar, K.N. Bhilegaonkar, Mithilesh Singh, Himani DhanzeAbstractJapanese encephalitis (JE) is a major public health problem in the South Asian countries including India. Pigs serve as a relevant sentinel model, the surveillance of which could predict a potential JE outbreak in human population nearby. However, existing serological detection methods like Enzyme-Linked Immuno Sorbent Assay (ELISA), virus neutralization test (VNT) and Haemagglutination Inhibition (HI) require elaborative labora...
Source: Biologicals - September 10, 2019 Category: Biology Source Type: research
Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
Publication date: Available online 6 September 2019Source: BiologicalsAuthor(s): Vinícius Augusto Simão, Caetano Pedro Evangelista-Ribeiro, Heloisa Brand, Lougan Lagass-Pereira, Laís Fernanda Marques, Pedro Henrique Benites-Aoki, Roseli Nunes da Silveira-Antunes, Aldo Tonso, João Tadeu Ribeiro-PaesAbstractAdipose-derived mesenchymal stromal/stem cells (ASC) have acquired a prominent role in tissue engineering and regenerative medicine. However, the standardization of basic culture procedures in this cellular type is still not well established according to the main qualitative cellular attributes. We evaluate the cell g...
Source: Biologicals - September 6, 2019 Category: Biology Source Type: research
The new China vaccine administration law: Re-establishing confidence in vaccines
Publication date: Available online 30 August 2019Source: BiologicalsAuthor(s): Baobin Huang (Source: Biologicals)
Source: Biologicals - August 31, 2019 Category: Biology Source Type: research
The duck EB66® cell substrate reveals a novel retrotransposon
Publication date: Available online 30 August 2019Source: BiologicalsAuthor(s): Fabien Perugi, Caroline Freslon-Evain, Luc Batard, Pauline Guillet, Klaus SchwambornAbstractDuring the establishment of the duck EB66® cell line as a new cell substrate for vaccine production in industry, a very low level of reverse transcriptase (RT) activity was detected in the culture supernatant by product-enhanced RT assay but a whole battery of tests failed to evidence infectious particles. Results from extensive biochemical and physical testing demonstrated that RT activity was associated to an intracellular, non-enveloped and dense stru...
Source: Biologicals - August 30, 2019 Category: Biology Source Type: research
Evaluation of a capture antigen ELISA for the characterisation of tetanus vaccines for veterinary use
Publication date: Available online 27 August 2019Source: BiologicalsAuthor(s): Rebecca Riches-Duit, Laura Hassall, Peter Rigsby, Paul StickingsAbstractWe previously developed an ELISA assay for detection of tetanus toxoid antigen in tetanus vaccines for human use. Tetanus vaccines for veterinary use are qualitatively different to those used in humans, often containing a larger number and variety of non-tetanus antigens in the multi-valent products, and adjuvants that are not found in human vaccines. We assessed performance of the capture ELISA with a range of veterinary tetanus vaccines as a first step towards development ...
Source: Biologicals - August 28, 2019 Category: Biology Source Type: research
A single immunogenicity assay for testing potency of combination DTaP vaccines: Simultaneous quantitation of anti-DT, anti-TT, anti-PTxd and anti-FHA antibodies in Guinea-pig serum with a Luminex®-xMAP® bead-based serological assay
Publication date: Available online 23 August 2019Source: BiologicalsAuthor(s): Silvio Bandiera, Annabelle Lebas, Livia Canizares-Martinello, Fabien Guinchard, Corinne Lyonnais, Sophie Perrin, Marine Nicolas, Sylvie Uhlrich, Martine Chabaud-RiouAbstractThe diphtheria toxoid (DT), tetanus toxoid (TT), and acellular pertussis (aP) single immunogenicity assay (DTaP SIA) is a Luminex®-xMAP®-bead-based multiplex immunoassay for estimating the potency of DTaP pediatric combination vaccines in guinea pigs. This manuscript describes the validation of this assay for the simultaneous quantitation of anti-diphtheria toxoid (anti-DT)...
Source: Biologicals - August 25, 2019 Category: Biology Source Type: research
Characterization of the viral genomes present in commercial batches of horse serum obtained by high-throughput sequencing
Publication date: Available online 23 August 2019Source: BiologicalsAuthor(s): W.P. Paim, M.N. Weber, S.P. Cibulski, M.S. da Silva, D.E. Puhl, R.F. Budaszewski, A.P.M. Varela, F.Q. Mayer, C.W. CanalAbstractHorses are often used as blood donors for commercial horse serum (HS) production and to manufacture biologicals. HS is an alternative for fetal bovine serum (FBS) used as a supplement for cell culture and vaccine production. Furthermore, HS is also frequently obtained in order to produce antisera toxins and pathogens. The advent of high-throughput sequencing (HTS) has promoted changes in virus detection, since previous k...
Source: Biologicals - August 23, 2019 Category: Biology Source Type: research
Qualification of a surface plasmon resonance assay to determine binding of IgG-type antibodies to complement component C1q
Publication date: Available online 21 August 2019Source: BiologicalsAuthor(s): Milan Jovic, Florian CymerAbstractInteraction of therapeutic antibodies with complement component C1q are frequently part of pharmaceutical characterization and production process comparability studies. Assays currently used to assess this interaction and/or the activation of the complement cascade are often cumbersome, time consuming or imprecise. We here report the further development, successful qualification and suitability evaluation of an SPR-based C1q binding assay for the characterization of IgG-C1q interactions. We evaluate different Ig...
Source: Biologicals - August 22, 2019 Category: Biology Source Type: research
Development of a novel chimeric PA-LF antigen of Bacillus anthracis, its immunological characterization and evaluation as a future vaccine candidate in mouse model
In this study, we developed a recombinant PA-LF chimera antigen of Bacillus anthracis by fusing the PA domain 2-4 with lethal factor (LF) domain 1 and evaluated its protective potential against B. anthracis in mouse model. The anti-PA-LF chimera serum reacted with both PA and LF antigen, individually. The chimera elicited a strong antibody titer in mice with predominance of IgG1 isotype followed by IgG2b, IgG2a and IgG3. Cytokines were assessed in splenocytes of immunized mice and a significant up-regulation in the expression of IL-4, IL-10, IFN-γ and TNF-α was observed. The PA-LF chimera immunized mice exhibited 80% sur...
Source: Biologicals - August 14, 2019 Category: Biology Source Type: research
Development of a new 15-valent pneumococcal conjugate vaccine (PCV15) and evaluation of its immunogenicity
In this study, we propose a new PCV15 candidate, in which the common 13 serotypes and a licensed control vaccine have equivalent efficacy while two additional serotypes showed adequate immunogenicity in the rabbit model. (Source: Biologicals)
Source: Biologicals - August 14, 2019 Category: Biology Source Type: research
Diagnostics in the veterinary field: The role in health surveillance and disease identification
Publication date: Available online 13 August 2019Source: BiologicalsAuthor(s): Anja Holm, Richard Hill, Attila Farsang, Carmen JungbäckAbstractAn international workshop, held in Wiesbaden, Germany on 15–17 May 2019 provided an overview of existing and new methods and approaches to diagnostics in animal health and their benefits and challenges. The variability in quality and authority review of test kits across the world is a concern for the reliability of test results and the decisions that are based on the diagnostic data. In countries or regions without regulatory oversight, there is an urgent need for international h...
Source: Biologicals - August 14, 2019 Category: Biology Source Type: research
High-throughput LC-MS quantitation of cell culture metabolites
In this study, we present a high-throughput LC-MS method with a 17-min cycle time that is capable of simultaneously monitoring 93 cell culture metabolites, including amino acids, nucleic acids, vitamins, sugars and others. This method has high precision and accuracy and has been successfully applied to the daily profiling of bioreactors and raw material qualification. Information obtained in these studies has been used to identify limiting amino acids during production, which guided adjustments to the feed strategy that prevented the potential misincorporation of amino acids. This type of metabolite profiling can be furt...
Source: Biologicals - August 7, 2019 Category: Biology Source Type: research
Establishing China's national standards of antigen content and neutralizing antibody responses for evaluation of SFTS vaccines
Publication date: Available online 27 July 2019Source: BiologicalsAuthor(s): Yuhua Li, Zheng Jia, Xiaohong Wu, Ling Wang, Lei Chen, Xinxian Dai, Xiuling Li, Junzhi WangAbstractSevere fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by severe fever with thrombocytopenia syndrome virus (SFTSV). SFTS is mainly characterized by severe fever with thrombocytopenia and has a high mortality rate. The virus has been found in China, South Korea, and Japan. Effective antiviral drugs or vaccines still have been unavailable. Now, two vaccine manufacturers in China are actively engaged in the development...
Source: Biologicals - July 28, 2019 Category: Biology Source Type: research
Prescription-based prevalence of biological therapy in patients with psoriasis, rheumatoid arthritis, and inflammatory bowel diseases
This study is based on data from IMS® Diagnosis Monitor and included patients who had received a biological drug in dermatology practices due to psoriasis (PSO), gastroenterology practices due to Crohn's disease (CD) or ulcerative colitis (UC), or rheumatology practices due to rheumatoid arthritis (RA) between April 2015 and December 2018. We analyzed 1,748,948 CD/UC-related prescriptions, 3,968,879 RA-related prescriptions, and 7,321,496 PSO-related prescriptions. Of these, 343,263 (19.6%) prescriptions for IBD, 92,343 (16.2%) prescriptions for RA, and 169,573 (6.9%) prescriptions for PSO were for biologicals. The propor...
Source: Biologicals - July 19, 2019 Category: Biology Source Type: research
Aims and Scope/Editorial Board/Publishing Details
Publication date: July 2019Source: Biologicals, Volume 60Author(s): (Source: Biologicals)
Source: Biologicals - July 12, 2019 Category: Biology Source Type: research
