Phosphatase inhibition increases AQP2 accumulation in the rat IMCD apical plasma membrane
In conclusion, PP1/PP2A regulates the phosphorylation and apical plasma membrane accumulation of AQP2 differently than PP2B. Serine-264 of AQP2 is a phosphorylation site that is regulated by both PP1/PP2A and PP2B. This dual regulatory pathway may suggest a previously unappreciated role for multiple phosphatases in the regulation of urine concentration. (Source: AJP: Renal Physiology)
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Ren, H., Yang, B., Ruiz, J. A., Efe, O., Ilori, T. O., Sands, J. M., Klein, J. D. Tags: ARTICLES Source Type: research
Mechanisms of connecting tubule glomerular feedback enhancement by aldosterone
Connecting tubule glomerular feedback (CTGF) is a mechanism where an increase in sodium (Na) concentration in the connecting tubule (CNT) causes the afferent arteriole (Af-Art) to dilate. We recently reported that aldosterone within the CNT lumen enhances CTGF via a nongenomic effect involving GPR30 receptors and sodium/hydrogen exchanger (NHE), but the signaling pathways of this mechanism are unknown. We hypothesize that aldosterone enhances CTGF via cAMP/protein kinase A (PKA) pathway that activates protein kinase C (PKC) and stimulates superoxide (O2–) production. Rabbit Af-Arts and their adherent CNTs were microd...
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Ren, Y., Janic, B., Kutskill, K., Peterson, E. L., Carretero, O. A. Tags: ARTICLES Source Type: research
Role of O-linked N-acetylglucosamine modification in diabetic nephropathy
In conclusion, hyperglycemia-induced elevation of O-GlcNAcylation contributes to the progression of DN via inhibition of Akt/eNOS phosphorylation and HSP72 induction. RAAS blockers successfully inhibit this process, suggesting a novel pathomechanism of their renoprotective action in the treatment of DN. (Source: AJP: Renal Physiology)
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Gellai, R., Hodrea, J., Lenart, L., Hosszu, A., Koszegi, S., Balogh, D., Ver, A., Banki, N. F., Fulop, N., Molnar, A., Wagner, L., Vannay, A., Szabo, A. J., Fekete, A. Tags: ARTICLES Source Type: research
What we need to know about the effect of lithium on the kidney
Lithium has been a valuable treatment for bipolar affective disorders for decades. Clinical use of lithium, however, has been problematic due to its narrow therapeutic index and concerns for its toxicity in various organ systems. Renal side effects associated with lithium include polyuria, nephrogenic diabetes insipidus, proteinuria, distal renal tubular acidosis, and reduction in glomerular filtration rate. Histologically, chronic lithium nephrotoxicity is characterized by interstitial nephritis with microcyst formation and occasional focal segmental glomerulosclerosis. Nevertheless, this type of toxicity is uncommon, wit...
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Gong, R., Wang, P., Dworkin, L. Tags: PERSPECTIVE Source Type: research
Functionomics of NCC mutations in Gitelman syndrome using a novel mammalian cell-based activity assay
In conclusion, we developed a mammalian cell-based assay in which NCC activity assessment together with a profiling of mutated protein processing aid our understanding of the pathogenic mechanism of the NCC mutations. (Source: AJP: Renal Physiology)
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Valdez-Flores, M. A., Vargas-Poussou, R., Verkaart, S., Tutakhel, O. A. Z., Valdez-Ortiz, A., Blanchard, A., Treard, C., Hoenderop, J. G. J., Bindels, R. J. M., Jelen, S. Tags: CALL FOR PAPERS Source Type: research
