Springer protocols feed by Biochemistry 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Electrochromatography on Acrylate-Based Monolith in Cyclic Olefin Copolymer Microchip: An Attractive Technology
Electrochromatography (EC) on a porous monolithic stationary phase prepared within the channels of a microsystem is an attractive alternative for on-chip separation. It combines the separation mechanisms of electrophoresis and liquid chromatography. Moreover, the porous polymer monolithic materials have become popular as stationary phase due to the ease and rapidity of fabrication via free radical photopolymerization. Here, we describe a hexyl acrylate (HA)-based porous monolith which is simultaneously in situ synthesized and anchored to the inner walls of the channel of a cyclic olefin copolymer (COC) device in only 2 min...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Carbon Nanotube-Based Separation Columns for Microchip Electrochromatography
Fabrication of the stationary phase for microchip chromatography is most often done by packing of the individual separation channel after fabrication of the microfluidic chip, which is a very time-consuming and costly process (Kutter. J Chromatogr A 1221:72–82, 2012). Here, we describe in detail the fabrication and operation protocols for devices with microfabricated carbon nanotube stationary phases for reverse-phase chromatography. In this protocol, the lithographically defined stationary phase is fabricated in the channel before bonding of a lid, thereby circumventing the difficult packaging procedures used in mor...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF Detection
Nonaqueous microchip electrophoresis (NAMCE), which makes use of an organic medium instead of a conventional aqueous buffer solution, is a promising separation method for analytical chemistry due to the enhanced solubility of hydrophobic analytes and tailored selectivity of separation. Here, we describe an NAMCE with LIF detection combined with a pump-free negative pressure sampling device for rapid determination of catecholamines (CAs) in urine samples, and the whole analysis time (including sampling time and separation time) was less than 1 min. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Fast and Continuous-Flow Detection and Separation of DNA Complexes and DNA in Nanofluidic Chip Format
Fast separation of DNA and detection of protein/DNA complexes are important in many state-of-the-art molecular medicine technologies, like the production of gene vaccines or medical diagnostics. Here, we describe a nanofluidic chip-based technique for fast, efficient, and virtually label-free detection and separation of protein/DNA and drug/DNA complexes and topological DNA variants. The mechanism is based on a continuous-flow dielectrophoresis at a nanoslit and allows efficient separation of small DNA fragments (<7,000 base pairs) and fast detection of DNA complexes within 1 min. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Sex Identification of Ancient DNA Samples Using a Microfluidic Device
Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Strepta...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Fast High-Throughput Screening of H1N1 Virus by Parallel Detection with Multichannel Microchip Electrophoresis
Influenza is one of the acute respiratory diseases of human caused by the influenza A (H1N1) virus and accounted for major public health concerns worldwide. The polymerase chain reaction (PCR) methods are the most popular tools for clinical diagnosis of influenza A virus. Microchip electrophoresis is a widely used method for DNA molecules separation. Herein, we describe the fast and high-throughput separation of hemagglutinin (HA) and nucleocapsid protein (NP) gene PCR products (116 bp and 195 bp, respectively) by parallel detection with multichannel microchip electrophoresis and programmed step electric field strength (PS...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation: Rapid Superparamagnetic-Beads-Based Automated Immunoseparation of Zn-Proteins from Staphylococcus aureus with Nanogram Yield
The emergence of drug-resistant bacteria and new or changing infectious pathogens is an important public health problem as well as a serious socioeconomic concern. Immunomagnetic separation-based methods create new possibilities for rapidly recognizing many of these pathogens. Nanomaterial-based techniques including fluorescent labeling by quantum dots as well as immunoextraction by magnetic particles are excellent tools for such purposes. Moreover, the combination with capillary electrophoresis in miniaturized microchip arrangement brings numerous benefits such as fast and rapid analysis, low sample consumption, very sens...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Fast Assembly of Anti-Voltage Photonic Crystals in Microfluidic Channels for Ultrafast Separation of Amino Acids and Peptides
Photonic crystals (PCs) with periodically ordered particle beds are ideal media for high-performance separation but hard to be stably and crack-freely assembled in various microfluidic channels. Here we describe a facile method to fast assemble crack-free and high-voltage-sustainable PCs into the micro channels. The key is to speed up an evaporation-induced assembly by heating up (at 70 °C) and blowing away the solvent vapor from one end of a channel and supplying silica suspension at the other end. Crack-free PCs can be prepared at a speed of 0.2 cm/min. The heat also accelerates the silica particles to gel with solve...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Multidimensional Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk Formula
Functional proteins have been found in infant milk formula as supplements, added by an increasing number of manufacturers. Their supplementations are expected to be controlled and monitored. Here, we describe a microchip-integrated CE method for the determination of these low levels of functional proteins in a protein-rich sample matrix. On-chip isoelectric focusing (IEF) is used to separate high-abundance proteins from low-abundance proteins instead of using some complicated time-consuming protein purification process. After that, transient isotachophoresis hyphenated capillary zone electrophoresis (t-ITP-CZE) can preconc...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Analysis of Ofloxacin in Ofloxacin Ear Drops by Microfluidic Chip Coupled with Contactless Conductivity Detection
A method using a microfluidic chip coupled with contactless conductivity detection (C4D) is demonstrated for the determination of ofloxacin in Ofloxacin Ear Drops. Settings, optimizing procedures, electrophoresis conditions, regression equations, and the average recovery rate are discussed. Under optimum conditions, the determination of ofloxacin in standard solution is achieved within 1 min, which allows detection of ofloxacin in Ofloxacin Ear Drops. The demonstrated method is rapid, high efficient, sensitive, and economical. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Analysis of Thiols by Microchip Capillary Electrophoresis for In Situ Planetary Investigations
Microchip capillary electrophoresis with laser-induced fluorescence detection (μCE-LIF) enables sensitive analyses of a wide range of analytes employing small volumes of sample and reagent (nL to μL) on an instrument platform with minimal mass, volume, and power requirements. This technique has been used previously in the analysis of amino acids and other organic molecules of interest in the fields of astrobiology and planetary science. Here, we present a protocol for the analysis of thiols using μCE-LIF. This protocol utilizes Pacific Blue C5-maleimide for fluorescent derivatization of thiols, enabling limits of ...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Microchip Capillary Electrophoresis of Nitrite and Nitrate in Cerebrospinal Fluid
Microchip capillary electrophoresis (MCE) is a relatively new analytical method requiring only small sample amounts, which is very favorable for the analysis of volume-limited biofluids. The practical use of MCE in bioanalysis is still restricted in terms of requirements for simplifying and/or concentrating sample pretreatment techniques. Here, we describe an MCE method for trace analysis of nitrite and nitrate, indicators of various neurological diseases, in cerebrospinal fluid (CSF). The complex CSF samples were simplified by solid-phase microextraction prior to an online combination of isotachophoresis with capillary zo...
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Microchip Electrophoresis for Fast and Interference-Free Determination of Trace Amounts of Glyphosate and Glufosinate Residues in Agricultural Products
Fast screening of herbicide residues is becoming important to ensure food safety, but traditional chromatographic methods may not be suitable for rapid on-site analysis of samples with complicated matrices. Here, we describe a method for rapid and sensitive determination of glyphosate (GLYP) and glufosinate (GLUF) residues in agricultural products by electrophoresis on disposable microchips with laser-induced fluorescence detection. With this method, quantitative analysis of trace amounts of GLYP and GLUF can be achieved with relatively simple sample preparation. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
An Overview of the Use of Microchips in Electrophoretic Separation Techniques: Fabrication, Separation Modes, Sample Preparation Opportunities, and On-Chip Detection
This chapter is intended as a basic introduction to microchip-based capillary electrophoresis to set the scene for newcomers and give pointers to reference material. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news
Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC Coupled with Electrochemical Detector
A highly sensitive, precise, and accurate reversed-phase high performance liquid-chromatography/electrochemical detection method for simultaneous determination of the endogenous free α-lipoic acid and dihydrolipoic acid in biological matrices is presented. The two analytes are extracted from samples with acetonitrile–10 % m-phosphoric acid solution(aqueous) (50:50 v/v). To determine the total lipoic acid, samples are treated with tris(2-carboxyethyl)phosphine solution in phosphate buffer: pH 2.5 with 85 % o-phosphoric acid prior to deproteination. The two analytes are separated on a C18 (150 ×&thin...
Source: Springer protocols feed by Biochemistry - October 20, 2014 Category: Biochemistry Source Type: news
