Springer protocols feed by Biochemistry 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
In nature, enzymes often form multienzyme complexes to enhance their catalytic efficiencies and, moreover, evolve into genetically fused multidomain enzymes. Inspired by a natural fusion cytochrome P450 (P450) containing a monooxygenase domain and a reductase domain, we have developed a heterotrimeric protein-utilized method to form a multienzyme complex composed of a bacterial P450 and its catalytically essential two redox proteins. Three distinct proliferating cell nuclear antigens (PCNAs) from Sulfolobus solfataricus, each of which can be separately expressed, spontaneously form a heterotrimer. Fusion to the PCNAs ...
Source: Springer protocols feed by Biochemistry - February 21, 2013 Category: Biochemistry Source Type: news
FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants
Methods for the cloning of large numbers of open reading frames (ORFs) into expression vectors are of critical importance for diverse disciplines in biology. Here I describe a system termed FX cloning that facilitates the high-throughput generation of expression constructs. FX cloning combines attractive features of established recombination- and single-strand-annealing-based cloning methods that were thus far not unified in one single method. FX cloning allows the straightforward transfer of a sequence-verified ORF to a variety of expression vectors, and it avoids the common but undesirable feature of significantly extend...
Source: Springer protocols feed by Biochemistry - February 21, 2013 Category: Biochemistry Source Type: news
Screening of Pirkle-Type Chiral Stationary Phases for HPLC Enantioseparations
The majority of enantiomeric separations for purity analysis and quality control continue to be performed by normal-phase liquid chromatography and supercritical fluid chromatography. In this chapter, representative chromatographic screening procedures for the enantioseparations using Pirkle-type stationary phases are presented. As Pirkle-type phases are commonly applied to the preparative chromatographic isolation of enantiomers, volatile modifiers are used in this screen in order to be subsequently compatible with the techniques used to recover analytes from preparative scale isolations. (Source: Springer protocols feed by Biochemistry)
Source: Springer protocols feed by Biochemistry - January 1, 2013 Category: Biochemistry Source Type: news
