Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF Detection

Nonaqueous microchip electrophoresis (NAMCE), which makes use of an organic medium instead of a conventional aqueous buffer solution, is a promising separation method for analytical chemistry due to the enhanced solubility of hydrophobic analytes and tailored selectivity of separation. Here, we describe an NAMCE with LIF detection combined with a pump-free negative pressure sampling device for rapid determination of catecholamines (CAs) in urine samples, and the whole analysis time (including sampling time and separation time) was less than 1 min.

Source: Springer protocols feed by Biochemistry - February 13, 2015 Category: Biochemistry Source Type: news