Genomics, Biology and Phylogeny Aurantiochytrium acetophilum sp. nov. (Thraustrochytriaceae), Including First Evidence of Sexual Reproduction
We described our new organism as Aurantiochytrium acetophilum sp. nov. (Thraustochytriaceae, Thraustocytriales) using light microscopy, electron microscopy, substrate assimilation, biochemical composition and nuclear genomic data. We found some characteristics of biotechnological relevance that were not previously described in this family. First, strain HS-399 of A. acetophilum was extremely tolerant to acetate toxicity, and it used this substrate as a sole carbon source. Second, we observed putative gametes that fused together to form a zygote. Zygote fate and the life stage with meiosis were not determined; however, we f...
Source: Protist - March 27, 2019 Category: Molecular Biology Source Type: research
Ploeotids Represent Much of the Phylogenetic Diversity of Euglenids
Publication date: Available online 23 March 2019Source: ProtistAuthor(s): Gordon Lax, Won Je Lee, Yana Eglit, Alastair SimpsonPloeotids are an assemblage of rigid phagotrophic euglenids that have 10–12 pellicular strips and glide on their posterior flagellum. Molecular phylogenies place them as a poorly resolved, likely paraphyletic assemblage outside the Spirocuta clade of flexible euglenids, which includes the well-known phototrophs and primary osmotrophs. Here, we report SSU rRNA gene sequences from 38 ploeotids, using both single-cell and culture-based methods. Several contain group I or non-canonical introns. Our ph...
Source: Protist - March 24, 2019 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: February 2019Source: Protist, Volume 170, Issue 1Author(s): (Source: Protist)
Source: Protist - March 21, 2019 Category: Molecular Biology Source Type: research
Discovery of a New Clade Nested Within the Genus Alexandrium (Dinophyceae): Morpho-molecular Characterization of Centrodinium punctatum (Cleve) F.J.R. Taylor
Publication date: Available online 28 February 2019Source: ProtistAuthor(s): Zhun Li, Kenneth Neil Mertens, Elisabeth Nézan, Nicolas Chomérat, Gwenael Bilien, Mitsunori Iwataki, Hyeon Ho ShinInvestigation of phytoplankton from East China Sea of the Pacific Ocean, offshore Réunion Island of the Indian Ocean, and the French Atlantic coast revealed a species of poorly known armored fusiform dinoflagellate. To clarify this species, morphology and phylogeny based on mitochondrial and nuclear protein gene sequence (Cox1, Cob and Hsp90) concatenated with the SSU, ITS region and LSU rDNA sequences were analysed. Epifluorescence...
Source: Protist - March 1, 2019 Category: Molecular Biology Source Type: research
Time Calibrated Morpho-molecular Classification of Nassellaria (Radiolaria)
Publication date: Available online 22 February 2019Source: ProtistAuthor(s): Miguel M. Sandin, Loic Pillet, Tristan Biard, Camille Poirier, Estelle Bigeard, Sarah Romac, Noritoshi Suzuki, Fabrice NotNassellaria are marine protists belonging to the Radiolaria lineage (Rhizaria). Their skeleton, made of opaline silica, exhibit an excellent fossil record, extremely valuable in micro-paleontological studies for paleo-environmental reconstruction. Yet, to date very little is known about the extant diversity and ecology of Nassellaria in contemporary oceans, and most of it is inferred from their fossil record. Here we present an...
Source: Protist - February 23, 2019 Category: Molecular Biology Source Type: research
Mapping the Single Origin of Replication in the Naegleria gruberi Extrachromosomal DNA Element
We reported elsewhere the molecular cloning and the complete sequence analysis of the entire rRNA gene-containing element of N. gruberi (strain EGB). Using neutral/neutral two-dimensional agarose electrophoresis, the region in the element enclosing the single replication origin using DNA from asynchronous and axenically propagated N. gruberi populations was localized within a 2.1 kbp fragment located approximately 2,300 bp from the 18S rRNA gene and 3,700 bp from the 28S rRNA gene. The results indicate that replication occurs from a single origin via a theta-type mode of replication rather than by a rolling circl...
Source: Protist - February 14, 2019 Category: Molecular Biology Source Type: research
Survival and growth in vitro of Paramoeba perurans populations cultured under different salinities and temperatures
Publication date: Available online 21 December 2018Source: ProtistAuthor(s): Catherine Collins, Malcolm Hall, Mark J. Fordyce, Patricia WhiteAbstractGrowth rates of Paramoeba perurans cultures under different temperature and salinity conditions were investigated in vitro over a 15 day period. Optimal population growth, under the experimental conditions, was observed at 15 °C and a salinity of 35‰, with amoebae populations doubling every 14 h. Positive P. perurans populations growth was observed at 15 °C between salinities of above 20‰ and 50‰, and at 8 °C, 11 °C and 18 °C at sa...
Source: Protist - December 22, 2018 Category: Molecular Biology Source Type: research
Tuberlatum coatsi gen. n., sp. n. (Alveolata, Perkinsozoa), a New Parasitoid with Short Germ Tubes Infecting Marine Dinoflagellates
Publication date: Available online 21 December 2018Source: ProtistAuthor(s): Boo Seong Jeon, Myung Gil ParkPerkinsozoa is an exclusively parasitic group within the alveolates and infections have been reported from various organisms, including marine shellfish, marine dinoflagellates, freshwater cryptophytes, and tadpoles. Despite its high abundance and great genetic diversity revealed by recent environmental rDNA sequencing studies, Perkinsozoa biodiversity remains poorly understood. During the intensive samplings in Korean coastal waters during June 2017, a new parasitoid of dinoflagellates was detected and was successful...
Source: Protist - December 21, 2018 Category: Molecular Biology Source Type: research
Cryptic Diversity: a Long-lasting Issue for Diatomologists
Publication date: February 2019Source: Protist, Volume 170, Issue 1Author(s): Alberto Amato, Wiebe H.C.F. Kooistra, Marina Montresor (Source: Protist)
Source: Protist - December 14, 2018 Category: Molecular Biology Source Type: research
Isosporan Oocysts in the faeces of bank Voles (Myodes glareolus; Arvicolinae, Rodentia): Real Parasites, or Pseudoparasites?
This study reveals the origin of isosporan oocysts found in faeces of bank voles based on morphological analyses, phylogenetic analyses, and experimental infections. Morphological analyses showed the presence of the Stieda body complex on sporocysts. Phylogenetic analyses demonstrated close phylogenetic relationships between Isospora from bank voles and avian isosporans. Experimental inoculations of bank voles with sporulated oocysts of Isospora did not result in the production of unsporulated oocysts. Hence, these organisms should be considered pseudoparasites of the bank voles/rodents (probably originating from avian Iso...
Source: Protist - December 12, 2018 Category: Molecular Biology Source Type: research
A Novel Phagomyxid Parasite Produces Sporangia in Root Hair Galls of Eelgrass (Zostera marina)
The objective of this study was to identify the parasite causing the formation of root hair galls on eelgrass (Zostera marina) in Puget Sound, WA. Microscopic and molecular analyses revealed that a novel protist formed plasmodia that developed into sporangia in root hair tip galls and released biflagellate swimming zoospores. Root hair galls were also observed in the basal section of root hairs, and contained plasmodia or formed thick-walled structures filled with cells (resting spores). Phylogenetic analyses of 18S rDNA sequence data obtained from cells in sporangia indicated that the closest relative of the parasite with...
Source: Protist - December 11, 2018 Category: Molecular Biology Source Type: research
Erratum to “The Uncoupled Assimilation of Carbon and Nitrogen from Urea and Glycine by the Bloom-forming Dinoflagellate Prorocentrum minimum” [Protist 169 (November (5)) (2018) 603–614]
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): Olga Matantseva, Ilya Pozdnyakov, Maren Voss, Iris Liskow, Sergei Skarlato (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research
Editorial Board
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research
Erratum to “Nutritional Intake by Ectoplasmic Nets of Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles” [Protist 169 (November (5)) (2018) 727–743]
Publication date: December 2018Source: Protist, Volume 169, Issue 6Author(s): Izumi Iwata, Daiske Honda (Source: Protist)
Source: Protist - December 5, 2018 Category: Molecular Biology Source Type: research
New Selectable Markers for Volvox carteri Transformation
Publication date: Available online 15 November 2018Source: ProtistAuthor(s): José A. Ortega-Escalante, Owen Kwok, Stephen M. MillerVolvox carteri is an excellent model for investigating the evolution of multicellularity and cell differentiation, and the rate of future progress with this system will depend on improved molecular genetic tools. Several selectable markers for nuclear transformation of V. carteri have been developed, including the nitrate reductase (nitA) gene, but it would be useful to have additional markers to multiplex transgenes in this species. To further facilitate molecular genetic analyses of V. carte...
Source: Protist - November 16, 2018 Category: Molecular Biology Source Type: research
