Grl1 Protein is a Candidate K Antigen in Tetrahymena thermophila
Publication date: Available online 27 March 2018 Source:Protist Author(s): Takahide Ota In Tetrahymena, K antigens associate only with mature basal bodies and are expected to play important roles in the morphogenesis and function of the membrane skeleton around basal bodies, but these proteins have not been identified and their functions are unknown. Commercially available anti-human Rho GDP-dissociation inhibitor α (RhoGDIα) antibody (sc-33201) was accidentally found to show very similar immunofluorescence staining patterns to those of anti-K antigen antibodies, such as 424A8 and 10D12 mouse monoclonal antibodies, in...
Source: Protist - March 27, 2018 Category: Molecular Biology Source Type: research
Morphology and Molecular Phylogeny of Peritrich Ciliate Epibionts on Pelagic Diatoms: Vorticella oceanica and Pseudovorticella coscinodisci sp. nov. (Ciliophora, Peritrichia)
Publication date: Available online 20 March 2018 Source:Protist Author(s): Fernando Gómez, Lu Wang, Senjie Lin Consortia of the peritrich ciliate Vorticella oceanica and the planktonic diatom Chaetoceros coarctactus are reported from the South Atlantic Ocean. The morphologies of the constituent species were similar to their counterparts from other geographical regions, revealing a widespread distribution in tropical waters. Besides, we describe Pseudovorticella coscinodisci sp. nov. that lives on Coscinodiscus wailesii and other pelagic diatoms off Brazil. The zooids were 34–45μm long, and 19–30μm wide with a s...
Source: Protist - March 21, 2018 Category: Molecular Biology Source Type: research
New Taxa of Streptophyte Algae (Streptophyta) from Terrestrial Habitats Revealed Using an Integrative Approach
Publication date: Available online 20 March 2018 Source:Protist Author(s): Tatiana Mikhailyuk, Alena Lukešová, Karin Glaser, Andreas Holzinger, Sabrina Obwegeser, Svetlana Nyporko, Thomas Friedl, Ulf Karsten Two new genera (Streptosarcina and Streptofilum) and three new species (Streptosarcina arenaria, S. costaricana and Streptofilum capillatum) of streptophyte algae were detected in cultures isolated from terrestrial habitats of Europe and Central America and described using an integrative approach. Additionally, a strain isolated from soil in North America was identified as Hormidiella parvula and proposed a...
Source: Protist - March 21, 2018 Category: Molecular Biology Source Type: research
Evaluation of Morphological Characteristics to Delineate Taxa of the Genus Trigonopyxis (Amoebozoa, Arcellinida)
Publication date: Available online 13 March 2018 Source:Protist Author(s): Garvin Schulz, Mark Maraun, Eckhard Völcker, Stefan Scheu, Valentyna Krashevska Morphological features are often the only characteristics suitable for identification of taxa in testate amoebae, especially in ecological and palaeoecological studies. However, whereas the morphology of some species is rather stable it may vary considerably in others. Within the order Arcellinida the genus Trigonopyxis with the type species Trigonopyxis arcula is morphologically highly variable. To identify reliable characteristics for morphology-based taxon del...
Source: Protist - March 14, 2018 Category: Molecular Biology Source Type: research
Unprecedented Symbiont Eukaryote Diversity Is Governed by Internal Trophic Webs in a Wild Non-Human Primate
Publication date: Available online 9 March 2018 Source:Protist Author(s): Justin J.S. Wilcox, Hope Hollocher Research on host-associated microbiomes has highlighted major divisions between the role of eukaryotes in free-living and symbiont systems. These trends call into question the relevance of macroecological processes to host-associated systems and the relative importance of parasitism, commensalism, and mutualism as evolutionary patterns across the domains of life. However, it is unclear as to whether these apparent differences reflect biological realities or methodologies in community characterization: free-livin...
Source: Protist - March 10, 2018 Category: Molecular Biology Source Type: research
Beat Blum Memoriam
Publication date: Available online 6 March 2018 Source:Protist Author(s): Larry Simpson (Source: Protist)
Source: Protist - March 8, 2018 Category: Molecular Biology Source Type: research
Collimyces mutans gen. et sp. nov. (Rhizophydiales, Collimycetaceae fam. nov.), a New Chytrid Parasite of Microglena (Volvocales, clade Monadinia)
Publication date: Available online 5 March 2018 Source:Protist Author(s): Kensuke Seto, Yousuke Degawa Chytrids are early diverging lineages of true fungi that reproduce with posteriorly uniflagellate zoospores. In aquatic ecosystems, parasitic chytrids of algae have important ecological roles by influencing the population dynamics of phytoplankton and transferring nutrients and energy from inedible algae to zooplankton via zoospores. Despite their ecological importance, information on parasitic chytrids is lacking in the current systematics of chytrids. Here, we investigated a novel chytrid culture KS100 that parasiti...
Source: Protist - March 6, 2018 Category: Molecular Biology Source Type: research
The Rainwater Rock-pool Dinoflagellate Nottbeckia ochracea gen. et comb. nov. (syn.: Hemidinium ochraceum) - A Fine-structural and Molecular Study with Emphasis on the Motile Stage
Publication date: Available online 24 February 2018 Source:Protist Author(s): Gert Hansen, Niels Daugbjerg, Øjvind Moestrup The rarely recorded dinoflagellate Hemidinium ochraceum Levander was isolated from a rainwater rock-pool in Finland and the motile stage studied by light- and electron microscopy and molecular sequencing. The ultrastructure of the motile stage revealed several features not previously observed in other dinoflagellates, e.g. thecal plates with inwards ‘knobs’ and a peculiar corrugated left sulcal plate. The apparent lack of the R2 and R4 flagellar roots was also unusual. The typical plate tabu...
Source: Protist - February 25, 2018 Category: Molecular Biology Source Type: research
The Hot Spot in a Cold Environment: Puzzling Parvodinium (Peridiniopsidaceae, Peridiniales) from the Polish Tatra Mountains
Publication date: Available online 23 February 2018 Source:Protist Author(s): Juliane Kretschmann, Paweł M. Owsianny, Anže Žerdoner Čalasan, Marc Gottschling Because of a great variety of remote localities and cold habitats, the Tatra Mountains are home to many freshwater protest lineages. Dinophytes have been subjected to a number of studies from this area dating mostly to the first half of the 20th century, but their true diversity remains elusive until today. We collected water tow samples at five lakes in the Tatra Mountains in order to establish monoclonal strains. We found four lineages that were distinctiv...
Source: Protist - February 24, 2018 Category: Molecular Biology Source Type: research
Phylogeny and Morphology of New Diplonemids from Japan
Publication date: Available online 21 February 2018 Source:Protist Author(s): Daria Tashyreva, Galina Prokopchuk, Akinori Yabuki, Binnypreet Kaur, Drahomíra Faktorová, Jan Votýpka, Chiho Kusaka, Katsunori Fujikura, Takashi Shiratori, Ken-Ichiro Ishida, Aleš Horák, Julius Lukeš Diplonemids were recently found to be the most species-rich group of marine planktonic protists. Based on phylogenetic analysis of 18S rRNA gene sequences and morphological observations, we report the description of new members of the genus Rhynchopus − R. humris sp. n. and R. serpens sp. n., and the establishment of two new gen...
Source: Protist - February 22, 2018 Category: Molecular Biology Source Type: research
Diversity and Distribution of Free-living Ciliates from High-Arctic Kara Sea Sediments
Publication date: Available online 12 February 2018 Source:Protist Author(s): Andrey I. Azovsky, Yuri A. Mazei For the first time, free-living ciliates were studied in sediments from a variety of habitats in the Kara Sea (the Arctic Ocean). Ciliates were found in a wide range of environmental conditions (from the intertidal zone up to depths of 554m, from salinities ranging from 0 to 34 psu, and from coarse sands and gravel to muddy sediments), with total abundances varying from 1 to 127 ind/cm3. Altogether, 114 species were identified, most of which were widely distributed and were previously reported from other Arcti...
Source: Protist - February 14, 2018 Category: Molecular Biology Source Type: research
Phylogenomic Analysis of Nassula variabilis n. sp., Furgasonia blochmanni, and Pseudomicrothorax dubius Confirms a Nassophorean Clade
Publication date: Available online 13 February 2018 Source:Protist Author(s): Denis H. Lynn, Martin Kolisko, William Bourland The class Nassophorea includes the microthoracids and nassulids, which share morphological similarities in their somatic kinetids and cytopharyngeal baskets. The monophyly of this clade has been challenged by small subunit rRNA gene sequences and multi-gene analyses that do not provide strong support. To provide a more robust test of the monophyly of the Nassophorea, phylogenomic analyses were based on 124 genes derived from the single-cell transcriptomes of the microthoracid Pseudomicrothorax ...
Source: Protist - February 14, 2018 Category: Molecular Biology Source Type: research
A Deep Hidden Diversity of Dictyostelia
Publication date: Available online 9 January 2018 Source:Protist Author(s): Sandra L. Baldauf, Maria Romeralo, Omar Fiz-Palacios, Nahid Heidari Dictyostelia is a monophyletic group of transiently multicellular (sorocarpic) amoebae, whose study is currently limited to laboratory culture. This tends to favour faster growing species with robust sorocarps, while species with smaller more delicate sorocarps constitute most of the group’s taxonomic breadth. The number of known species is also small (∼150) given Dictyostelia’s molecular depth and apparent antiquity (>600 myr). Nonetheless, dictyostelid sequence...
Source: Protist - January 23, 2018 Category: Molecular Biology Source Type: research
A Re-investigation of Sarcinochrysis marina (Sarcinochrysidales, Pelagophyceae) from its Type Locality and the Descriptions of Arachnochrysis, Pelagospilus, Sargassococcus and Sungminbooa genera nov.
Publication date: Available online 11 January 2018 Source:Protist Author(s): Kwi Young Han, Louis Graf, Carolina P. Reyes, Barbara Melkonian, Robert A. Andersen, Hwan Su Yoon, Michael Melkonian Systematists increasingly use molecular markers to identify species; however, most microalgae were described before gene sequencing and type specimens were often ink drawings. Cryptic speciation and biogeographic isolation are other potential problems when anchoring an old species name with a modern gene sequence. Therefore when biological type material is absent, the best approach is to recollect the alga from the type loc...
Source: Protist - January 23, 2018 Category: Molecular Biology Source Type: research
Symbiont Replacement Between Bacteria of Different Classes Reveals Additional Layers of Complexity in the Evolution of Symbiosis in the Ciliate Euplotes
Publication date: Available online 20 December 2017 Source:Protist Author(s): Vittorio Boscaro, Sergei I. Fokin, Giulio Petroni, Franco Verni, Patrick J. Keeling, Claudia Vannini Symbiosis is a diverse and complex phenomenon requiring diverse model systems. The obligate relationship between a monophyletic group of Euplotes species (“clade B”) and the betaproteobacteria Polynucleobacter and “Candidatus Protistobacter” is among the best-studied in ciliates, and provides a framework to investigate symbiont replacements. Several other Euplotes-bacteria relationships exist but are less understood, such as the co...
Source: Protist - December 21, 2017 Category: Molecular Biology Source Type: research
