Rediscovery of the Testate Amoeba Genus Penardeugenia (Thaumatomonadida, Imbricatea)
We describe two new species from Germany, P. huelsmannii and P. hausmannii. (Source: Protist)
Source: Protist - December 20, 2017 Category: Molecular Biology Source Type: research
Ploidy and Number of Chromosomes in the Alveolate Alga Chromera velia
Publication date: Available online 18 December 2017 Source:Protist Author(s): Jan Vazač, Zoltán Füssy, Irena Hladová, Sireesha Killi, Miroslav Oborník Chromera velia is an alveolate alga which represents the closest known phototrophic relative to apicomplexan parasites. Although the nuclear, mitochondrial, and plastid genomes of this alga have been sequenced, the number of chromosomes and ploidy of C. velia are unknown. We explored ploidy in the vegetative cell, the predominant stage in cultures of Chromera, using the tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH) in isolated nuclei...
Source: Protist - December 19, 2017 Category: Molecular Biology Source Type: research
Evidence of Subpopulations with Distinct Biological Features Within a Leishmania (Viannia) braziliensis Strain
Publication date: Available online 5 December 2017 Source:Protist Author(s): Léa Cysne-Finkelstein, Mariana Silva-Almeida, Bernardo Acácio Santini Pereira, Karen dos Santos Charret, Álvaro Luiz Bertho, Leonardo Soares Bastos, Luzia de Oliveira Pinto, Francisco Odêncio Rodrigues de Oliveira, Mirian Cláudia da Souza Pereira, Carlos Roberto Alves The present study demonstrates that the Leishmania (Viannia) braziliensis strain MCAN/BR/1998/R619 is composed of multiple subpopulations with measurable distinctions. Single parasites were separated from a culture of promastigotes in stationary phase by cell sorting...
Source: Protist - December 6, 2017 Category: Molecular Biology Source Type: research
Eyes of the Deep-sea Floor: The Integrative Taxonomy of the Foraminiferal Genus Vanhoeffenella
Publication date: Available online 26 November 2017 Source:Protist Author(s): Ivan Voltski, Andrew J. Gooday, Jan Pawlowski Vanhoeffenella is a common deep-sea monothalamous foraminifer, some species of which have a unique eye-like test morphology. Owing to its world-wide distribution, it has been recorded numerous times since the “heroic age” of the deep-sea exploration in the early 20th century. So far, only 4 species have been described, and no attempts have been made to estimate the real diversity of this peculiar genus. Over the last fifteen years, we have collected specimens of Vanhoeffenella from various de...
Source: Protist - November 27, 2017 Category: Molecular Biology Source Type: research
A New Classification of the Dictyostelids
Publication date: Available online 24 November 2017 Source:Protist Author(s): Sanea Sheikh, Mats Thulin, James C. Cavender, Ricardo Escalante, Shin-ichi Kawakami, Carlos Lado, John C. Landolt, Vidyanand Nanjundiah, David C. Queller, Joan E. Strassmann, Frederick W. Spiegel, Steven L. Stephenson, Eduardo M. Vadell, Sandra L. Baldauf Traditional morphology-based taxonomy of dictyostelids is rejected by molecular phylogeny. A new classification is presented based on monophyletic entities with consistent and strong molecular phylogenetic support and that are, as far as possible, morphologically recognizable. Al...
Source: Protist - November 24, 2017 Category: Molecular Biology Source Type: research
The Chytrid-like Parasites of Algae Amoeboradix gromovi gen. et sp. nov. and Sanchytrium tribonematis Belong to a New Fungal Lineage
Publication date: Available online 21 November 2017 Source:Protist Author(s): Sergey A. Karpov, Purificación López-García, Maria A. Mamkaeva, Vladimir I. Klimov, Andrey E. Vishnyakov, Victoria S. Tcvetkova, David Moreira Fungi encompass, in addition to classically well-studied lineages, an ever-expanding diversity of poorly known lineages including zoosporic chytrid-like parasites. Here, we formally describe Amoeboradix gromovi gen. et sp. nov. comprising a set of closely related strains of chytrid-like parasites of the yellow-green alga Tribonema gayanum unusually endowed with amoeboid zoospores. Morphological...
Source: Protist - November 22, 2017 Category: Molecular Biology Source Type: research
Tetrahymena Cilia Cap is Built in a Multi-step Process: A Study by Atomic Force Microscopy
Publication date: Available online 20 October 2017 Source:Protist Author(s): Cecília Seixas, João Gonçalves, Luís Viseu Melo, Helena Soares Cilia are complex and dynamic organelles that have motility and sensory functions. Defects in cilia biogenesis and function are at the origin of human ciliopathies. In motile cilia, a basal body organizes the axoneme composed of nine microtubule doublets surrounding a central pair of singlet microtubules. The distal ends of axonemal microtubules are attached to the membrane by microtubule-capping structures. Little is known about the early steps of cilium assembly. Although c...
Source: Protist - November 1, 2017 Category: Molecular Biology Source Type: research
Establishment of a de novo Reference Transcriptome of Histomonas meleagridis Reveals Basic Insights About Biological Functions and Potential Pathogenic Mechanisms of the Parasite
Publication date: Available online 6 October 2017 Source:Protist Author(s): Rounik Mazumdar, Lukas Endler, Andreas Monoyios, Michael Hess, Ivana Bilic The flagellate protozoan Histomonas meleagridis is the causative agent of histomonosis in poultry. In turkeys, high mortality might be noticed whereas in chickens the disease is less severe despite production losses. Discovered over a century ago, molecular data on this parasite are scarce and genetic studies are in its infancy. To expand genomic information, a de novo transcriptome sequencing of H. meleagridis was performed from a virulent and an attenuated strain, c...
Source: Protist - October 7, 2017 Category: Molecular Biology Source Type: research
Novel Specificity of IDO Enzyme Involved in the Biosynthesis of Mating Pheromone in the Ciliate Blepharisma stoltei
Publication date: Available online 18 September 2017 Source:Protist Author(s): Mayumi Sugiura, Hajime J. Yuasa, Terue Harumoto Mating pheromones (gamone 1 and gamone 2) in the ciliate Blepharisma are biologically active substances that trigger sexual reproduction (conjugation) under starvation conditions. Gamone 1 is a glycoprotein secreted by type I cells, and gamone 2 is a tryptophan (Trp)-derivative compound secreted by type II cells. Both gamones stimulate complementary mating type cells to promote each gamone production and induce pair formation. To elucidate the biosynthetic pathway of gamone 2, we investigated ...
Source: Protist - September 19, 2017 Category: Molecular Biology Source Type: research
Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation
Publication date: Available online 14 September 2017 Source:Protist Author(s): Eva Bínová, David Bína, David A. Ashford, Jane Thomas-Oates, Eva Nohýnková The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pse...
Source: Protist - September 16, 2017 Category: Molecular Biology Source Type: research
Revision of the Genus Micromonas Manton et Parke (Chlorophyta, Mamiellophyceae), of the Type Species M. pusilla (Butcher) Manton & amp; Parke and of the Species M. commoda van Baren, Bachy and Worden and Description of Two New Species Based on the Genetic and Phenotypic Characterization of Cultured Isolates
Publication date: Available online 14 September 2017 Source:Protist Author(s): Nathalie Simon, Elodie Foulon, Daphné Grulois, Christophe Six, Yves Desdevises, Marie Latimier, Florence Le Gall, Margot Tragin, Aude Houdan, Evelyne Derelle, Fabien Jouenne, Dominique Marie, Sophie Le Panse, Daniel Vaulot, Birger Marin The green picoalgal genus Micromonas is broadly distributed in estuaries, coastal marine habitats and open oceans, from the equator to the poles. Phylogenetic, ecological and genomic analyses of culture strains and natural populations have suggested that this cosmopolitan genus is composed of se...
Source: Protist - September 16, 2017 Category: Molecular Biology Source Type: research
Morphological and Molecular Characterization of Aggregata spp. Frenzel 1885 (Apicomplexa: Aggregatidae) in Octopus vulgaris Cuvier 1797 (Mollusca: Cephalopoda) from Central Mediterranean
Publication date: Available online 11 September 2017 Source:Protist Author(s): Perla Tedesco, Camino Gestal, Kristina Begić, Ivona Mladineo, Sheila Castellanos-Martínez, Gaetano Catanese, Antonio Terlizzi, Graziano Fiorito Coccidian parasites of the genus Aggregata are known to parasitize cephalopods as definitive hosts, however one of the genus members, A. octopiana, has shown an unresolved phylogeny within the same definitive host, the common octopus (Octopus vulgaris). Our study represents a large-scale investigation aimed at characterizing morphological traits and phylogeny of A. octopiana isolated from O. ...
Source: Protist - September 12, 2017 Category: Molecular Biology Source Type: research
Ultrastructure and Phylogeny of Kirithra asteri gen. et sp. nov. (Ceratoperidiniaceae, Dinophyceae) − a Free-Living, Thin-Walled Marine Photosynthetic Dinoflagellate from Argentina
Publication date: Available online 9 September 2017 Source:Protist Author(s): Pernille Vængebjerg Boutrup, Øjvind Moestrup, Urban Tillmann, Niels Daugbjerg A gymnodinioid photosynthetic dinoflagellate was isolated from Argentina and examined by light and electron microscopy and analysis of nuclear-encoded LSU rDNA. Kirithra asteri gen. et sp. nov. was proposed as morphology and molecular phylogeny separated this dinoflagellate from others within the family Ceratoperidiniaceae. Cells were surrounded by a hyaline amphiesma comprising polygonal vesicles. Each vesicle contained a honeycomb and a trilaminar structure. A...
Source: Protist - September 9, 2017 Category: Molecular Biology Source Type: research
Diversification and Phylogenetics of Mobilid Peritrichs (Ciliophora) with Description of Urceolaria parakorschelti sp. nov.
Publication date: August 2017 Source:Protist, Volume 168, Issue 4 Author(s): Nicholas A.T. Irwin, Mona Sabetrasekh, Denis H. Lynn Ciliates within the order Mobilida comprise a group of ectosymbionts that form ecologically and economically important symbioses with aquatic organisms. However, despite their significance, the factors driving mobilid diversification are poorly understood. To address this, we sought to examine the genetic diversity of two mobilid families, the trichodinids and urceolariids, over geographical areas and from different hosts. To this end we collected and provide morphological and molecular cha...
Source: Protist - August 19, 2017 Category: Molecular Biology Source Type: research
Corrigendum to “Revision of the Genus Cryptomonas (Cryptophyceae): A Combination of Molecular Phylogeny and Morphology Provides Insights into a Long-Hidden Dimorphism” [Protist 154 (2003) 371–409]
Publication date: August 2017 Source:Protist, Volume 168, Issue 4 Author(s): Kerstin Hoef-Emden, Michael Melkonian (Source: Protist)
Source: Protist - August 18, 2017 Category: Molecular Biology Source Type: research
