Stat5a KO(Stat5a tm1Mam) Mouse
Stat 5a Knockout: Stat5a deficiency results in the loss of prolactin-dependent mammary gland development and lactogenesis.Prolactin induces mammary gland development and lactogenesis. Binding of Prolactin to its receptor leads to the phosphorylation and activation of STAT (signal transducers and activators of transcription) proteins. Two Stat proteins, Stat 5a and Stat5b, are expressed in mammary tissues during pregnancy. Stat5a null mice developed normally, and were indistinguishable from hemizygous and wild-type littermates in size, weight and fertility. Mammary lobulo-alveolar outgrowth during pregnancy was reduced and ...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Stat1LoxP (Stat1 tm1Mam) Mouse
Selective inactivation of Stat 1 in mammary cells indicates that its effect as a tumor suppressor in breast is direct.STAT1 is considered a tumor suppressor, but it is not known if this effect occurs directly in mammary cells or secondarily by disrupting interferon signaling through the JAK/STAT1 pathway to induce immune responses. ERBB2/neu-induced breast cancer appeared sooner in mice lacking STAT1 only in mammary cells than in wild-type mice, indicating that STAT1 tumor suppression was intrinsic to mammary cells and not secondary to an induced immune response. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Stat1LoxP (Stat1 tm1Mam) Mouse
Selective inactivation of Stat 1 in mammary cells indicates that its effect as a tumor suppressor in breast is direct.STAT1 is considered a tumor suppressor, but it is not known if this effect occurs directly in mammary cells or secondarily by disrupting interferon signaling through the JAK/STAT1 pathway to induce immune responses. ERBB2/neu-induced breast cancer appeared sooner in mice lacking STAT1 only in mammary cells than in wild-type mice, indicating that STAT1 tumor suppression was intrinsic to mammary cells and not secondary to an induced immune response. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Sphk2 KO Mouse
A knockout mouse for Sphingosine kinase 2 (Sphk2).Sphingosine kinase 1 and 2 are enzymes that produce sphingosine-1-phosphate (S1P), a potent bioactive compound that activates a family of G-protein coupled receptors known as S1P receptors. Triggering these receptors on cells has important effects related to inflammation, immunity, cancer, angiogenesis, cell proliferation, adhesion, cardiovascular function, nervous system function and injury responses. Sphingosine kinase 1 is the predominant isozyme for extracellular S1P production. Sphingosine kinase 2 is the predominant isozyme that activates fingolimod (brand name: Gilen...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Sphk2 KO Mouse
A knockout mouse for Sphingosine kinase 2 (Sphk2).Sphingosine kinase 1 and 2 are enzymes that produce sphingosine-1-phosphate (S1P), a potent bioactive compound that activates a family of G-protein coupled receptors known as S1P receptors. Triggering these receptors on cells has important effects related to inflammation, immunity, cancer, angiogenesis, cell proliferation, adhesion, cardiovascular function, nervous system function and injury responses. Sphingosine kinase 1 is the predominant isozyme for extracellular S1P production. Sphingosine kinase 2 is the predominant isozyme that activates fingolimod (brand name: Gilen...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Sphk1 KO Mouse
A Sphingosine kinase 1 (Sphk1) knockout mouse.Sphingosine kinase 1 and 2 are enzymes that produce sphingosine-1-phosphate (S1P), a potent bioactive compound that activates a family of G-protein coupled receptors known as S1P receptors. Triggering these receptors on cells has important effects related to inflammation, immunity, cancer, angiogenesis, cell proliferation, adhesion, cardiovascular function, nervous system function and injury responses. Sphingosine kinase 1 is the predominant isozyme for extracellular S1P production. Sphingosine kinase 2 is the predominant isozyme that activates Fingolimod (brand name: Gilenya??...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Sphk1 KO Mouse
A Sphingosine kinase 1 (Sphk1) knockout mouse.Sphingosine kinase 1 and 2 are enzymes that produce sphingosine-1-phosphate (S1P), a potent bioactive compound that activates a family of G-protein coupled receptors known as S1P receptors. Triggering these receptors on cells has important effects related to inflammation, immunity, cancer, angiogenesis, cell proliferation, adhesion, cardiovascular function, nervous system function and injury responses. Sphingosine kinase 1 is the predominant isozyme for extracellular S1P production. Sphingosine kinase 2 is the predominant isozyme that activates Fingolimod (brand name: Gilenya??...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Small volume viscometer
NIDDK scientists have developed a capillary-based device and system for measuring the rheological properties of solutions of synthetic and biological polymers. The device automatically serially dilutes and varies the flow rate of a sample, permitting measurement of solution viscosity across wide ranges of concentration and shear rate without changing samples. Viscosity is calculated directly from Poiseuille's law, given the measured difference in pressure between two ends of a capillary tube through which the solution is flowing at a known rate. The device can rapidly and accurately assay solute stability, solute-solvent a...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Single electrode corona discharge electrochemical/electrospray ionization (U.S. Patent Number 7,759,643)
A single electrode electrochemical/electrospray ionization source using a corona discharge and a method of analyzing a sample using a corona discharge single electrode electrochemical/electrospray ionization source are provided. In the corona discharge single electrode electrochemical/electrospray ionization technique electrons are removed from the metal tip of the device through gases present in the electrospray ion source resulting in electrochemical ionization of the sample of interest. The resulting odd electron sample cation (positive ion mode) or anion (negative ion mode) can then be analyzed by an appropriate techni...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Simple, rapid and reliable method for detecting thalassemia (U.S. Patent Number 5,281,519)
A simple, rapid and reliable method for diagnosis of thalassemia is described. The method comprises amplification of the cDNA by polymerase chain reaction and determining the ratio between α and β hemoglobin chain mRNAs. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
S1pr3 KO Mouse
A Spingosine-1-phosphate Receptor-3 (S1pr3) knockout mouse.Sphingosine-1-phosphate (S1P), a sphingolipid metabolite, activates cell signaling by interacting with a family of G-protein-coupled cell-surface S1P receptors that activate different and overlapping signaling pathways. Three S1P receptors (S1P1, S1P2 and S1P3) are abundant in embryonic endothelial cells. SIP1 knockouts are embryonic lethals because of hemorrhage, but neither S1P2 null mice nor S1P3 null mice were embryonic lethals. Double null embryos (S1P2 S1P3) and triple null embryos (S1P1 S1P2 S1P3), however, displayed a more severe vascular phenotype than did...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
S1pr3 KO Mouse
A Spingosine-1-phosphate Receptor-3 (S1pr3) knockout mouse.Sphingosine-1-phosphate (S1P), a sphingolipid metabolite, activates cell signaling by interacting with a family of G-protein-coupled cell-surface S1P receptors that activate different and overlapping signaling pathways. Three S1P receptors (S1P1, S1P2 and S1P3) are abundant in embryonic endothelial cells. SIP1 knockouts are embryonic lethals because of hemorrhage, but neither S1P2 null mice nor S1P3 null mice were embryonic lethals. Double null embryos (S1P2 S1P3) and triple null embryos (S1P1 S1P2 S1P3), however, displayed a more severe vascular phenotype than did...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
S1pr2 KO Mouse
A Spingosine-1-phosphate Receptor-2 (S1pr2) knockout mouse.Sphingosine-1-phosphate (S1P), a sphingolipid metabolite, activates cell signaling by interacting with a family of G-protein-coupled cell-surface S1P receptors that activate different and overlapping signaling pathways. Three S1P receptors (S1P1, S1P2 and S1P3) are abundant in embryonic endothelial cells. S1P1 knockouts are embryonic lethals because of hemorrhage. S1P2 null mice did not exhibit embryonic lethality or phenotypic abnormalities. However, double null embryos (S1P1 S1P2 and S1P2 S1P3), and triple null embryos (S1P1 S1P2 S1P3) displayed a more severe vas...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
S1pr2 KO Mouse
A Spingosine-1-phosphate Receptor-2 (S1pr2) knockout mouse.Sphingosine-1-phosphate (S1P), a sphingolipid metabolite, activates cell signaling by interacting with a family of G-protein-coupled cell-surface S1P receptors that activate different and overlapping signaling pathways. Three S1P receptors (S1P1, S1P2 and S1P3) are abundant in embryonic endothelial cells. S1P1 knockouts are embryonic lethals because of hemorrhage. S1P2 null mice did not exhibit embryonic lethality or phenotypic abnormalities. However, double null embryos (S1P1 S1P2 and S1P2 S1P3), and triple null embryos (S1P1 S1P2 S1P3) displayed a more severe vas...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
S1pr1 LoxP (S1pr1 tm2Rlp) Mouse
A conditional knockout mouse for S1pr1Sphingosine-1-phosphate (S1P) is a potent bioactive lipid that activates a family of G-protein coupled receptors, S1P1, S1P2, S1P3, S1P4 and S1P5. The S1P1 receptor is widely expressed and has critical functions in the immune, cardiovascular and nervous systems. It is a target for Fingolimod (brand name: Gilenya???), an immunomodulating drug, FDA approved for treating multiple sclerosis. The S1pr1 knockout mouse and the S1pr1 floxed mouse are useful for studying the function of the S1P1 receptor.Mice with a floxed S1P1 receptor gene were mated with mice expressing Cre-recombinase only ...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
