UTX LoxP Mouse
UTX-flox. Conditional knockout mice for the histone demethylase UTX (Kdm6a) conditional knockout will help understand its role as a tumor suppressor.Di- and tri-methylations on histone H3 lysine 27 (H3K27me2 and H3K27me3) are epigenetic marks for gene repression. UTX (ubiquitously transcribed X chromosome protein), also known as Kdm6a (lysine (K)-specific demethylase 6a) is a histone demethylase that specifically removes H3K27me2 and H3K27me3. UTX knockout mice are embryonic lethal, so we have generated UTX conditional knockout mice (UTX-flox) in which exon 24 is flanked with loxP sites. UTX has been found to be a tumor su...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
UTX LoxP Mouse
UTX-flox. Conditional knockout mice for the histone demethylase UTX (Kdm6a) conditional knockout will help understand its role as a tumor suppressor.Di- and tri-methylations on histone H3 lysine 27 (H3K27me2 and H3K27me3) are epigenetic marks for gene repression. UTX (ubiquitously transcribed X chromosome protein), also known as Kdm6a (lysine (K)-specific demethylase 6a) is a histone demethylase that specifically removes H3K27me2 and H3K27me3. UTX knockout mice are embryonic lethal, so we have generated UTX conditional knockout mice (UTX-flox) in which exon 24 is flanked with loxP sites. UTX has been found to be a tumor su...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Use of nitrite salts for the treatment of cardiovascular conditions (U.S. Patent Application Number 20070154569 A1 )
It has been surprisingly discovered that administration of nitrite to subjects causes a reduction in blood pressure and an increase in blood flow to tissues. The effect is particularly beneficial, for example, to tissues in regions of low oxygen tension. This discovery provides useful treatments to regulate a subject's blood pressure and blood flow, for example, by the administration of nitrite salts. Provided herein are methods of administering a pharmaceutically-acceptable nitrite salt to a subject, for treating, preventing or ameliorating a condition selected from: (a) ischemia-reperfusion injury (e.g., hepatic or cardi...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Use of leptin for treating human lipoatrophy and method of determining predisposition to said treatment (U.S. Patent Application Number 20070099836)
Leptin, leptin analogs, and leptin derivatives are used to treat patients with lipoatrophy. Leptin is effective against conditions of lipoatrophy for both genetic and acquired forms of the disease. A therapeutically effective amount of leptin can be administered in a variety of ways, including subcutaneously and using gene therapy methods. Methods of the present invention contemplate administration of leptin, leptin analogs, and leptin derivatives to patients having a leptin level of approximately 4 ng/ml or less before treatment. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Use of homo sapiens chromosome 1 open reading frame 28 (c1orf28) in the diagnosis of hyperparathyroidism-jaw tumor syndrome (U.S. Patent Application Number WO 2003094860 A3)
The present invention provides an isolated or purified oligonucleotide consisting essentially of the nucleotide sequence of Clorf28 and comprising a mutation; a fragment of the oligonucleotide; a vector comprising the oligonucleotide or fragment thereof; a cell comprising the vector; an isolated or purified polypeptide encoded by the above oligonucleotide or fragment thereof; a method of detecting HPT-JT or a predisposition to HPT-JT in a human, which method comprises detecting either at least one mutation in a nucleic acid comprising the nucleotide sequence of C1orf28 or a mutant C1orf28 protein; and an antibody which bin...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Transframe peptide inhibitor of viral protease (U.S. Patent Number 5,872,210)
The present invention describes small, water soluble peptides isolated from a native virus inhibitory sequence that blocks maturation of the virally encoded protease and inhibits the mature protease as well. The peptides may be used in the treatment of virally infected cells, in the preparation of vaccine formulations, in the generation of clinically relevant antibodies and anti-idiotypic antibodies and in the generation of a screening assay or kit that can be used to identify other similarly acting protease inhibitors. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Therapeutic Polypeptides and Their Use (U.S. Patent Application Number 61/890,585)
NIDDK investigators have developed a synthetic polypeptide with beneficial effects and prospective treatment of chronic kidney disease (CKD). End stage renal disease (ESRD) is a major health and economic burden and requires transplantation or dialysis to avoid imminent death. ESRD is the final stage of CKD, which progresses over the course of decades. The major causes of CKD are diabetes and hypertension, and treatments for diabetes and hypertension are only partially effective to slow the progression of CKD. Only one class of drugs Angiotensin Converting Enzyme inhibitors and/or Angiotensin Receptor Blockers (ACEi/ARB) is...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Therapeutic Polypeptides and Their Use (U.S. Patent Application Number 61/890,585)
NIDDK investigators have developed a synthetic polypeptide with beneficial effects and prospective treatment of chronic kidney disease (CKD). End stage renal disease (ESRD) is a major health and economic burden and requires transplantation or dialysis to avoid imminent death. ESRD is the final stage of CKD, which progresses over the course of decades. The major causes of CKD are diabetes and hypertension, and treatments for diabetes and hypertension are only partially effective to slow the progression of CKD. Only one class of drugs Angiotensin Converting Enzyme inhibitors and/or Angiotensin Receptor Blockers (ACEi/ARB) is...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Tg(Wap-cre)11738Mam Mouse
Cre-recombinase under the control of the whey acidic acid protein was only detected in alveolar epithelial epithelial cells of mammary tissue during lactation, and transcription occurred at all stages of mammary development.The Cre recombinase from bacteriophage P1 excises intervening DNA sequences located between two unidirectional lox sites positioned on the same linear DNA segment, leaving one lox site behind. Through insertion of lox sites via homologous recombination into the gene of interest and targeting Cre recombinase expression to a specific cell type using a tissue-specific promoter, it is possible to introduce ...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Tg(Wap-cre)11738Mam Mouse
Cre-recombinase under the control of the whey acidic acid protein was only detected in alveolar epithelial epithelial cells of mammary tissue during lactation, and transcription occurred at all stages of mammary development.The Cre recombinase from bacteriophage P1 excises intervening DNA sequences located between two unidirectional lox sites positioned on the same linear DNA segment, leaving one lox site behind. Through insertion of lox sites via homologous recombination into the gene of interest and targeting Cre recombinase expression to a specific cell type using a tissue-specific promoter, it is possible to introduce ...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Tg(MMTV-Cre)#Mam Mouse
Cre-recombinase under the control of mouse mammary tumor virus long terminal repeat (MMTV) was expressed in the salivary gland and mammary epithelial cells of adult mice, and induced recombination in all tissues.The Cre recombinase from bacteriophage P1 excises intervening DNA sequences located between two unidirectional lox sites positioned on the same linear DNA segment, leaving one lox site behind. Through insertion of lox sites via homologous recombination into the gene of interest and targeting Cre recombinase expression to a specific cell type using a tissue-specific promoter, it is possible to introduce predetermine...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Tg(MMTV-Cre)#Mam Mouse
Cre-recombinase under the control of mouse mammary tumor virus long terminal repeat (MMTV) was expressed in the salivary gland and mammary epithelial cells of adult mice, and induced recombination in all tissues.The Cre recombinase from bacteriophage P1 excises intervening DNA sequences located between two unidirectional lox sites positioned on the same linear DNA segment, leaving one lox site behind. Through insertion of lox sites via homologous recombination into the gene of interest and targeting Cre recombinase expression to a specific cell type using a tissue-specific promoter, it is possible to introduce predetermine...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Synthesis and purification of hepatitis C virus-like particles (U.S. Patent Number 6,387,662)
Production of enveloped RNA virus-like particles intracellularly in vitro in insect cells using a recombinant baculovirus vector containing a cDNA coding for viral structural proteins is disclosed. In vitro production and purification of hepatitis C virus (HCV)-like particles containing HCV core protein, E1 protein and E2 protein is disclosed. Production of antibodies in vivo to the purified HCV-like particles is disclosed. (Source: NIDDK Research Resources)
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Stat5a LoxP/Stat5b LoxP (Stat5a/Stat5b tm2Mam) Mouse
Conditional knockout of Stat5a and Stat5b: Combined deletion of conserved Stat5a and Stat5b in mammary epithelium at different times during pregnancy reveal multiple distinct functions.The signal transducer and activator of transcription (STAT) family of transcription factors conveys signals from membrane receptors to the nucleus, where they activate diverse genetic programs. Stat5a and Stat5b are highly conserved proteins that are activated by many cytokines, erythropoietin, prolactin and growth hormone. Despite their similarities, they have many unique functions. Stat5a deficiency results in the loss of prolactin-depende...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
Stat5a KO(Stat5a tm1Mam) Mouse
Stat 5a Knockout: Stat5a deficiency results in the loss of prolactin-dependent mammary gland development and lactogenesis.Prolactin induces mammary gland development and lactogenesis. Binding of Prolactin to its receptor leads to the phosphorylation and activation of STAT (signal transducers and activators of transcription) proteins. Two Stat proteins, Stat 5a and Stat5b, are expressed in mammary tissues during pregnancy. Stat5a null mice developed normally, and were indistinguishable from hemizygous and wild-type littermates in size, weight and fertility. Mammary lobulo-alveolar outgrowth during pregnancy was reduced and ...
Source: NIDDK Research Resources - February 16, 2017 Category: Endocrinology Source Type: research
