Chapter 3 - The Application of Molecular Methods Towards an Understanding of the Role of the Vaginal Microbiome in Health and Disease
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Joanna-Lynn C. Borgogna, Carl J. YeomanAbstractThe human vagina harbours one of the most ecologically interesting and important microbial ecosystems of our holobiome. The healthy human vagina is typically dominated by Lactobacillus species. Therein, vaginal lactobacilli provide the first line of protection against potential pathogens and, consequently, in the protection and maintenance of female gynecologic and reproductive health. Although much of our understanding of this system was elucidated from early studies and the application of traditional ...
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Series Page
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Contributors
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Preface
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Colin Harwood (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Chapter 1 - Bioinformatics in Microbiome Analysis
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Yuhan Hao, Zhiheng Pei, Stuart M. BrownAbstractDiseases driven by pathogens have been studied for decades, but the mechanisms of those diseases are still not completely understood. Next-generation sequencing provides a powerful technology with which to observe changes of pathogens at a genome level and the composition of microbial communities. This in turn has facilitated investigation on the associations between the microbiome and diseases. This chapter first will discuss bioinformatics applications under the “one pathogen to one disease” parad...
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Cryo-Electron Microscopy of Extremely Halophilic Microbes
Publication date: Available online 20 June 2018Source: Methods in MicrobiologyAuthor(s): H. Engelhardt, D. BollschweilerAbstractHalophilic and extremely halophilic microorganisms in particular have been a challenge for conventional electron microscopy (EM) as well as for state-of-the-art cryo-EM and cryo-electron tomography (cryo-ET). Conventional preparation techniques such as negative staining of isolated macromolecular complexes and plastic embedding of whole cells for ultrathin sectioning suffer from the problem of eliminating most of the salt without destroying the biological samples. Cryo-EM on the other hand, which ...
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Methods for Collection and Characterization of Samples From Icy Environments
Publication date: Available online 20 June 2018Source: Methods in MicrobiologyAuthor(s): Manuel Bedrossian, Jody W. Deming, Jay NadeauAbstractEnvironments where the prevailing temperature is below 0°C include both solid (frozen) substrates, such as glacier ice and permafrost, and liquid environments such as cold springs and cryopegs. Some of these environments have low solute concentrations, whereas others have very high levels of salts and other solutes, with corresponding freezing point depression. Some have high microbial density, while others represent the limits of cell survival. All of these present specific challen...
Source: Methods in Microbiology - July 11, 2018 Category: Microbiology Source Type: research
Chapter 3 - Microfluidics for bacterial imaging
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): L.E. Eland, A. Wipat, S. Lee, S. Park, L.J. WuAbstractMicrofluidics offers the technology for creating and maintaining microenvironments that is much needed for the microscopic study of bacteria. Over the past decade, microfluidics has been used in an increasingly large number of research studies, resulting in many important insights and discoveries. This chapter highlights some recent applications of microfluidic microscopy for bacteria, ranging from research in the engineering of bacterial systems, multispecies biofilm and microbial ecology, bacte...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Index
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Series Page
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Contributors
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Preface
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): Colin Harwood, Grant J. Jensen (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Chapter 1 - Methods for visualization of peptidoglycan biosynthesis
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): Y.-P. Hsu, X. Meng, M.S. VanNieuwenhzeAbstractPeptidoglycan is a rigid envelope surrounding the cytoplasmic membrane of most bacterial species. It helps protect bacterial cells from environmental stress and helps preserve cell morphology throughout their life cycle. Peptidoglycan biosynthesis is also an important regulator of bacterial cell division. Since the discovery of penicillin, it has also been an important drug target for antibacterial discovery and development. As a result, a significant effort has been directed at expanding our knowledge o...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Chapter 2 - Time-lapse microscopy and image analysis of Escherichia coli cells in mother machines
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): Y. Yang, X. Song, A.B. LindnerAbstractHigh-throughput longitudinal measurements of single cells by automated time-lapse microscopy, combined with dynamic system modelling, have in recent decades advanced our understanding of microbial physiology, such as cell size control, gene expression and regulation, subcellular organisation and organelle dynamics, development and cell fate decision. However, the sheer large numbers of cells resulting from exponential growth often limit both the experimental duration and numbers of single cells quantified. To ad...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Chapter 4 - Electron cryotomography
Publication date: 2016Source: Methods in Microbiology, Volume 43Author(s): C.M. Oikonomou, M.T. Swulius, A. Briegel, M. Beeby, Q. Yao, Y.-W. Chang, G.J. JensenAbstractElectron cryotomography (ECT) delivers 3D images of native structures inside intact cells with resolution on the scale of large macromolecular complexes (~ 4 nm). It has proven to be an invaluable tool for interrogating the organization of bacterial cells and the structures of the nanomachines inside them. Here we present a brief introduction to the technique, a few examples of what we have learned by imaging bacterial cells with ECT over the last 1...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
