Chapter 2 - A Review of the Oesophageal Microbiome in Health and Disease
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Dervla Kelly, Liying Yang, Zhiheng PeiAbstractMicrobial members of the gastrointestinal tract, known as the GTI microbiome, are beneficial for host metabolism and digestion, thereby creating a symbiotic relationship with their host (Petersen & Round, 2014). The microbiome has recently emerged as contributing to many intestinal disease states such as gastrointestinal reflux disease, Barrett's oesophagus and oesophageal cancers. Given that the local bacterial population influences individuals’ disease susceptibility, the microbiome of the gastrointe...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Chapter 3 - The Application of Molecular Methods Towards an Understanding of the Role of the Vaginal Microbiome in Health and Disease
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Joanna-Lynn C. Borgogna, Carl J. YeomanAbstractThe human vagina harbours one of the most ecologically interesting and important microbial ecosystems of our holobiome. The healthy human vagina is typically dominated by Lactobacillus species. Therein, vaginal lactobacilli provide the first line of protection against potential pathogens and, consequently, in the protection and maintenance of female gynecologic and reproductive health. Although much of our understanding of this system was elucidated from early studies and the application of traditional ...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Series Page
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Contributors
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Preface
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Colin Harwood (Source: Methods in Microbiology)
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Chapter 1 - Bioinformatics in Microbiome Analysis
Publication date: 2017Source: Methods in Microbiology, Volume 44Author(s): Yuhan Hao, Zhiheng Pei, Stuart M. BrownAbstractDiseases driven by pathogens have been studied for decades, but the mechanisms of those diseases are still not completely understood. Next-generation sequencing provides a powerful technology with which to observe changes of pathogens at a genome level and the composition of microbial communities. This in turn has facilitated investigation on the associations between the microbiome and diseases. This chapter first will discuss bioinformatics applications under the “one pathogen to one disease” parad...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Cryo-Electron Microscopy of Extremely Halophilic Microbes
Publication date: Available online 20 June 2018Source: Methods in MicrobiologyAuthor(s): H. Engelhardt, D. BollschweilerAbstractHalophilic and extremely halophilic microorganisms in particular have been a challenge for conventional electron microscopy (EM) as well as for state-of-the-art cryo-EM and cryo-electron tomography (cryo-ET). Conventional preparation techniques such as negative staining of isolated macromolecular complexes and plastic embedding of whole cells for ultrathin sectioning suffer from the problem of eliminating most of the salt without destroying the biological samples. Cryo-EM on the other hand, which ...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Methods for Collection and Characterization of Samples From Icy Environments
Publication date: Available online 20 June 2018Source: Methods in MicrobiologyAuthor(s): Manuel Bedrossian, Jody W. Deming, Jay NadeauAbstractEnvironments where the prevailing temperature is below 0°C include both solid (frozen) substrates, such as glacier ice and permafrost, and liquid environments such as cold springs and cryopegs. Some of these environments have low solute concentrations, whereas others have very high levels of salts and other solutes, with corresponding freezing point depression. Some have high microbial density, while others represent the limits of cell survival. All of these present specific challen...
Source: Methods in Microbiology - July 5, 2018 Category: Microbiology Source Type: research
Cryo-Electron Microscopy of Extremely Halophilic Microbes
Publication date: Available online 20 June 2018 Source:Methods in Microbiology Author(s): H. Engelhardt, D. Bollschweiler Halophilic and extremely halophilic microorganisms in particular have been a challenge for conventional electron microscopy (EM) as well as for state-of-the-art cryo-EM and cryo-electron tomography (cryo-ET). Conventional preparation techniques such as negative staining of isolated macromolecular complexes and plastic embedding of whole cells for ultrathin sectioning suffer from the problem of eliminating most of the salt without destroying the biological samples. Cryo-EM on the other hand, which is...
Source: Methods in Microbiology - June 21, 2018 Category: Microbiology Source Type: research
Methods for Collection and Characterization of Samples From Icy Environments
Publication date: Available online 20 June 2018 Source:Methods in Microbiology Author(s): Manuel Bedrossian, Jody W. Deming, Jay Nadeau Environments where the prevailing temperature is below 0°C include both solid (frozen) substrates, such as glacier ice and permafrost, and liquid environments such as cold springs and cryopegs. Some of these environments have low solute concentrations, whereas others have very high levels of salts and other solutes, with corresponding freezing point depression. Some have high microbial density, while others represent the limits of cell survival. All of these present specific challeng...
Source: Methods in Microbiology - June 21, 2018 Category: Microbiology Source Type: research
Chapter 3 The Application of Molecular Methods Towards an Understanding of the Role of the Vaginal Microbiome in Health and Disease
Publication date: 2017 Source:Methods in Microbiology, Volume 44 Author(s): Joanna-Lynn C. Borgogna, Carl J. Yeoman The human vagina harbours one of the most ecologically interesting and important microbial ecosystems of our holobiome. The healthy human vagina is typically dominated by Lactobacillus species. Therein, vaginal lactobacilli provide the first line of protection against potential pathogens and, consequently, in the protection and maintenance of female gynecologic and reproductive health. Although much of our understanding of this system was elucidated from early studies and the application of traditional mi...
Source: Methods in Microbiology - October 19, 2017 Category: Microbiology Source Type: research
Chapter 2 A Review of the Oesophageal Microbiome in Health and Disease
Publication date: 2017 Source:Methods in Microbiology, Volume 44 Author(s): Dervla Kelly, Liying Yang, Zhiheng Pei Microbial members of the gastrointestinal tract, known as the GTI microbiome, are beneficial for host metabolism and digestion, thereby creating a symbiotic relationship with their host (Petersen & Round, 2014). The microbiome has recently emerged as contributing to many intestinal disease states such as gastrointestinal reflux disease, Barrett's oesophagus and oesophageal cancers. Given that the local bacterial population influences individuals’ disease susceptibility, the microbiome of the gas...
Source: Methods in Microbiology - October 19, 2017 Category: Microbiology Source Type: research
Chapter 1 Bioinformatics in Microbiome Analysis
Publication date: 2017 Source:Methods in Microbiology, Volume 44 Author(s): Yuhan Hao, Zhiheng Pei, Stuart M. Brown Diseases driven by pathogens have been studied for decades, but the mechanisms of those diseases are still not completely understood. Next-generation sequencing provides a powerful technology with which to observe changes of pathogens at a genome level and the composition of microbial communities. This in turn has facilitated investigation on the associations between the microbiome and diseases. This chapter first will discuss bioinformatics applications under the “one pathogen to one disease” paradi...
Source: Methods in Microbiology - October 19, 2017 Category: Microbiology Source Type: research
The Application of Molecular Methods Towards an Understanding of the Role of the Vaginal Microbiome in Health and Disease
Publication date: Available online 12 October 2017 Source:Methods in Microbiology Author(s): Joanna-Lynn C. Borgogna, Carl J. Yeoman The human vagina harbours one of the most ecologically interesting and important microbial ecosystems of our holobiome. The healthy human vagina is typically dominated by Lactobacillus species. Therein, vaginal lactobacilli provide the first line of protection against potential pathogens and, consequently, in the protection and maintenance of female gynecologic and reproductive health. Although much of our understanding of this system was elucidated from early studies and the application ...
Source: Methods in Microbiology - October 13, 2017 Category: Microbiology Source Type: research
Bioinformatics in Microbiome Analysis
Publication date: Available online 10 October 2017 Source:Methods in Microbiology Author(s): Yuhan Hao, Zhiheng Pei, Stuart M. Brown Diseases driven by pathogens have been studied for decades, but the mechanisms of those diseases are still not completely understood. Next-generation sequencing provides a powerful technology with which to observe changes of pathogens at a genome level and the composition of microbial communities. This in turn has facilitated investigation on the associations between the microbiome and diseases. This chapter first will discuss bioinformatics applications under the “one pathogen to one ...
Source: Methods in Microbiology - October 11, 2017 Category: Microbiology Source Type: research
