Porcine survival model to simulate acute upper gastrointestinal bleedings
The existing animal models used for the simulation of acute gastrointestinal bleedings are usually non-survival models. We developed and evaluated a new porcine model (domestic pig, German Landrace) in which the animal remains alive and survives the artificial bleeding without any cardiovascular impairment. This consists of a bleeding catheter which is implanted into the stomach, then subcutaneously tunnelled from the abdomen to the neck where it is exteriorized and fixed with sutures. Using the injection of porcine blood, controllable and reproducible acute upper gastrointestinal bleeding can be simulated while maintainin...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Prosst, R. L., Schurr, M. O., Schostek, S., Krautwald, M., Gottwald, T. Tags: Short Reports Source Type: research
Transcranial direct current stimulation in the male mouse to promote recovery after stroke
Transcranial direct current stimulation (tDCS) constitutes a promising approach for promoting recovery of function after stroke, although the underlying neurobiological mechanisms are unclear. To conduct translational research in animal models, stimulation parameters should not lead to neuronal lesions. Liebetanz et al. recommend charge densities for cathodal stimulation in rats, but parameters for mice are not established. We established tDCS in the wild-type mouse, enabling studies with genetically-engineered mice (GEM). tDCS equipment was adapted to fit the mouse skull. Using different polarities and charge densiti...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Pikhovych, A., Walter, H. L., Mahabir, E., Fink, G. R., Graf, R., Schroeter, M., Rueger, M. A. Tags: Short Reports Source Type: research
Alfaxalone-butorphanol versus alfaxalone-morphine combination for immersion anaesthesia in oriental fire-bellied toads (Bombina orientalis)
Oriental fire-bellied toads (Bombina orientalis) are small semi-aquatic anuran species popular as both pets and laboratory animals. Although they are commonly anaesthetized to undergo clinical and experimental procedures, very little is known about their anaesthetic management. The aims of this prospective, randomized, cross-over experimental trial were to establish effective butorphanol and morphine concentrations to be added to alfaxalone for immersion anaesthesia (pilot study), and to compare the anaesthetic and antinociceptive effects of the two drug mixtures (alfaxalone–butorphanol and alfaxalone–morphine)...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Adami, C., dOvidio, D., Casoni, D. Tags: Original Articles Source Type: research
Maternal and fetal arterial blood gas data during general anaesthesia for caesarean delivery of preterm twin lambs
Much remains to be understood with regards the effects of prolonged anaesthesia on maternal and fetal haemodynamics and oxygenation. With the aim of improving anaesthetic management of pregnant sheep undergoing recovery surgery under anaesthesia, paired maternal and fetal arterial blood samples were collected during caesarean delivery of twin preterm lambs to document the blood gas status of the ewe and fetus. Twenty-one Merino twin pregnant ewes at 126 (±1) days of gestation were anaesthetized for caesarean delivery of their fetuses. Arterial blood samples were collected from the radial artery of the ewe and umbili...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Musk, G. C., Kemp, M. W. Tags: Original Articles Source Type: research
Inhalation anesthesia of rats: influence of the fraction of inspired oxygen on limb ischemia/reperfusion injury
Inhalation anesthesia with isoflurane is a well-established and safe method used in small laboratory animals. In most cases oxygen is used as a carrier gas for isoflurane, but room air or mixtures of oxygen with air or nitrous oxide are also being used. Anesthesia is therefore administered using different fractions of inspired oxygen (FiO2), and this may have consequences for the outcome of experiments. The aim of the present study was to investigate the influence of FiO2 on rat hind limb ischemia/reperfusion injury and to refine the used inhalation anesthesia. Male Wistar rats were subjected to 3.5 h of ischemia and 2 h o...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Zhang, S., Duehrkop, C., Plock, J., Rieben, R. Tags: Original Articles Source Type: research
The effects of pentobarbital, ketamine-pentobarbital and ketamine-xylazine anesthesia in a rat myocardial ischemic reperfusion injury model
To achieve reliable experimental data, the side-effects of anesthetics should be eliminated. Since anesthetics exert a variety of effects on hemodynamic data and incidence of arrhythmias, the selection of anesthetic agents in a myocardial ischemic reperfusion injury model is very important. The present study was performed to compare hemodynamic variables, the incidence of ventricular arrhythmias, and infarct size during 30 min of ischemia and 120 min of reperfusion in rats using pentobarbital, ketamine–pentobarbital or ketamine–xylazine anaesthesia. A total of 30 rats were randomly divided into three groups. In...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Shekarforoush, S., Fatahi, Z., Safari, F. Tags: Original Articles Source Type: research
The scent of stress: environmental challenge in the peripartum environment of mice affects emotional behaviours of the adult offspring in a sex-specific manner
Early adverse experiences are known to influence the risk of developing psychiatric disorders later. To shed further light on the development of laboratory mice, we systematically examined the influence of a prenatal or postnatal olfactory stressor, namely unfamiliar male mouse faeces, presented to pregnant or nursing mouse dams. Maternal and offspring behaviours were then examined. Maternal behaviours relative to controls revealed changes in nest building by the pregnant dams exposed to the unfamiliar faeces. There were no differences among groups on pup retrieval or exploration by the dams. Behavioural phenotyping of mal...
Source: Laboratory Animals - May 11, 2016 Category: Research Authors: Lerch, S., Dormann, C., Brandwein, C., Gass, P., Chourbaji, S. Tags: Original Articles Source Type: research
Translated abstracts, in French, German and Spanish
(Source: Laboratory Animals)
Source: Laboratory Animals - May 11, 2016 Category: Research Tags: Translated abstracts Source Type: research
News from Rus-LASA
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Authors: Krasilshchikova, M. Tags: News Source Type: research
1st iberian congress for laboratory animal sciences
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Authors: Reyes Panadero, M. Tags: News Source Type: research
Directive 2010/63/EU: facilitating full and correct implementation
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Authors: Chlebus, M., Guillen, J., Prins, J.-B. Tags: News Source Type: research
FELASA 2016 program
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Tags: News Source Type: research
Brussels Revisited
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Tags: News Source Type: research
News from the editors
(Source: Laboratory Animals)
Source: Laboratory Animals - April 17, 2016 Category: Research Authors: Riederer, B. M., Rodgers, J., Bomzon, A., Baehler, N. Tags: News Source Type: research
Environmental complexity and feeding enrichment can mitigate effects of space constraints in captive callitrichids
We examined the effects of enclosure types of differing size and environmental complexity on the activities of two species of callitrichids. We found that cotton-top tamarins housed in an enclosure of larger size and more environmental complexity showed higher activity levels, which was mainly contributed by more feeding/foraging activity. By contrast, Goeldi’s monkeys housed in an enclosure of larger size and more environmental complexity showed lower activity levels, which was mainly contributed by less locomotory activity. In both species, off-exhibit groups housed in smaller enclosures did not show significantly ...
Source: Laboratory Animals - April 17, 2016 Category: Research Authors: Sha, J. C. M., Ismail, R., Marlena, D., Lee, J. L. Tags: Original Articles Source Type: research
