Journal of Molecular Catalysis B: Enzymatic Journal of Molecular Catalysis B: Enzymatic RSS feedThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Bridging the bridge —Stabilization of CalB against H2O2 and its application in epoxidation reactions
Publication date: Available online 5 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Max Steinhagen, Anna Gräbner, Janine Meyer, Angelika E.W. Horst, Anja Drews, Dirk Holtmann, Marion B. Ansorge-Schumacher Chemo-enzymatic reactions combine the advantages of chemistry and biocatalysis. Chemical synthesis provides precursor molecules and low costs, whereas the application of enzymes provides selectivity. Additionally, the mild reaction conditions of enzymatic approaches allow the chemical application of intermediates and/or products that otherwise are not accessible. One example is the in...
Source: Journal of Molecular Catalysis B: Enzymatic - January 17, 2017 Category: Biochemistry Source Type: research

Modification and characterization of natural aluminosilicates, expanded perlite, and its application to immobilise α – amylase from A. oryzae.
Publication date: Available online 18 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): J. Rodriguez, F. Soria, H. Geronazzo, H. Destefanis The aim of this study was to modify natural silicoaluminates as expanded perlite (EP) through simple and inexpensive treatments in order to get a greater reactivity of surface and immobilise α-amylase from A. oryzae by adsorption and covalent binding. The materials obtained (expanded perlite treated with HCl, rehydroxylated expanded perlite, zeolite, and the incorporation of polystyrene in expanded perlite) were analysed by infrared spectroscopy, thermal...
Source: Journal of Molecular Catalysis B: Enzymatic - January 17, 2017 Category: Biochemistry Source Type: research

The immobilisation of proteases produced by SSF onto functionalized magnetic nanoparticles: Application in the hydrolysis of different protein sources
Publication date: Available online 16 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Noraziah Abu Yazid, Raquel Barrena, Antoni Sánchez Alkaline proteases produced from protein-rich waste (hair waste and soya residues) by solid state fermentation (SSF) were immobilised onto functionalized magnetic iron oxide nanoparticles (MNPs) using glutaraldehyde as a crosslinking agent. The covalent binding method had a better immobilisation yield compared to simple adsorption, retaining 93%-96% (459±106U/mg nanoparticles, 319±34U/mg nanoparticles) of hair waste and soya residues proteases, respectiv...
Source: Journal of Molecular Catalysis B: Enzymatic - January 16, 2017 Category: Biochemistry Source Type: research

Biocatalytic study of novel oleate hydratases
Publication date: Available online 16 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Jens Schmid, Lisa Steiner, Silvia Fademrecht, Jürgen Pleiss, Konrad B. Otte, Bernhard Hauer The direct hydration of CC bonds to yield alcohols or the reverse dehydration is chemically challenging but highly sought after. Recently, oleate hydratases (OAHs) gained attention as biocatalytic alternatives capable of hydrating isolated, non-activated CC bonds. Their natural reaction is the conversion of oleic acid to (R)-10-hydroxystearic acid. In this work, we report the first comparative study of several OA...
Source: Journal of Molecular Catalysis B: Enzymatic - January 16, 2017 Category: Biochemistry Source Type: research

Catalase immobilized-radiation grafted functional cellulose matrix: A novel biocatalytic system
Publication date: Available online 4 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Nilanjal Misra, N.K. Goel, S.A. Shelkar, L. Varshney, Virendra Kumar The work highlights a unique, one step protocol for room temperature immobilization of enzyme catalase onto epoxy functionalised cellulose matrix developed via gamma radiation induced simultaneous irradiation grafting of Glycidylmethacrylate (GMA). Effect of grafting parameters, such as radiation dose, monomer concentration and solvent composition on the grafting yield, was studied in order to optimize the radiation grafting process. Poly...
Source: Journal of Molecular Catalysis B: Enzymatic - January 13, 2017 Category: Biochemistry Source Type: research

Structural and catalytic alteration of sarcosine oxidase through reconstruction with coenzyme-like ligands
Publication date: Available online 13 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Yu Xin, Mengling Zheng, Qing Wang, Liushen Lu, Ling Zhang, Yanjun Tong, Wu Wang A sarcosine oxidase (SOX) gene from Bacillus sp. (AY626822.2) was expressed in Escherichia coli BL21 (DE3) in the form of inclusion bodies. A 3D model of SOX was then built and refined, and molecular docking was used to investigate the interactions between SOX and natural or coenzyme-like ligands, including flavin adenine dinucleotide (FAD); flavin mononucleotide (FMN); riboflavin; isoalloxazine; 7-methyl-8-chloro-10-(1′-D...
Source: Journal of Molecular Catalysis B: Enzymatic - January 12, 2017 Category: Biochemistry Source Type: research

Cloning, expression and characterization of two thermostable esterases from Aquifex aeolicus VF5
In this study, two novel esterases were identified in hyperthermophilic bacterium Aquifex aeolicus VF5 based on the typical characteristics of lipolytic enzymes. Two genes, Aaeo1 and Aaeo2 encoded 207 and 226 amino acids respectively, were synthesized after optimization according to the P. pastoris codon preferences. Using sequence alignment and phylogenetic analysis, Aaeo1 and Aaeo2, were classified into the bacterial lipolytic enzyme family VIII and V, respectively. Two genes were cloned and expressed in P. pastoris cells, and the recombinant enzymes were purified from culture supernatants by Ni2+-NTA affinity chromatogr...
Source: Journal of Molecular Catalysis B: Enzymatic - January 9, 2017 Category: Biochemistry Source Type: research

Synthesis of butyl esters via ultrasound-assisted transesterification of maca úba (Acrocomia aculeata) acid oil using a biomass-derived fermented solid as biocatalyst
Publication date: Available online 9 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Jakeline K.P. Todeschini, Erika C.G. Aguieiras, Aline M. de Castro, Marta A.P. Langone, Denise M.G. Freire, Rafael C. Rodrigues Enzymatic synthesis of fatty acid esters from macaúba (Acrocomia aculeata) acid oil was studied in a solvent-free system using a fermented and dry babassu (Orbygnia oleifera) cake with lipase activity from Rhizomucor miehei, named solid enzymatic preparation (SEP). The reactions were carried out under the influence of ultrasound irradiation and using different alcohols. Higher e...
Source: Journal of Molecular Catalysis B: Enzymatic - January 8, 2017 Category: Biochemistry Source Type: research

Search and discovery of actinobacteria capable of transforming deoxycholic and cholic acids
Publication date: Available online 26 December 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): N.O. Deshcherevskaya, T.G. Lobastova, V.V. Kollerov, A.V. Kazantsev, M.V. Donova The capability of 54 selected actinobacteria strains of different phyla to convert deoxycholic (DCA) and cholic (CA) acids under aerobic conditions was studied. Except for the two species, the strains did not grow on DCA (1g/l) as a sole carbon source, but some of them effectively converted DCA performing 7β- and 9α- hydroxylation, 3α- and 12α-dehydrogenation, partial cleavage of the isoprenoic side chain and Δ4-dehydro...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Enzymatic synthesis of dioctyl sebacate
Publication date: Available online 26 December 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Igor Nascentes dos Santos Corrêa, Sabrina Spagnolo Pedro da Silva, Danielle Simas de Queiroz, Danielle Oliveira Rosas, Marta Antunes Pereira Langone Pollution and environmental conservation have increased visibility in the global context over past decades. Specifically, regarding lubricants market, concern has been raised due to the large amount of this material released to the environment. In this scenario, development of biodegradable lubricants was investigated. One of the most important class of bio...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Catalase immobilized-radiation grafted functional cellulose matrix: A novel biocatalytic
Publication date: Available online 4 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Nilanjal Misra, N.K. Goel, S.A. Shelkar, L. Varshney, Virendra Kumar The work highlights a unique, one step protocol for room temperature immobilization of enzyme catalase onto epoxy functionalised cellulose matrix developed via gamma radiation induced simultaneous irradiation grafting of Glycidylmethacrylate (GMA). Effect of grafting parameters, such as radiation dose, monomer concentration and solvent composition on the grafting yield, was studied in order to optimize the radiation grafting process. Poly...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Bridging the Bridge − Stabilization of CalB against H2O2 and its Application in Epoxidation Reactions
Publication date: Available online 5 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Max Steinhagen, Anna Gräbner, Janine Meyer, Angelika E.W. Horst, Anja Drews, Dirk Holtmann, Marion B. Ansorge-Schumacher Chemo-enzymatic reactions combine the advantages of chemistry and biocatalysis. Chemical synthesis provides precursor molecules and low costs, whereas the application of enzymes provides selectivity. Additionally, the mild reaction conditions of enzymatic approaches allow the chemical application of intermediates and/or products that otherwise are not accessible. One example is the in...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Peroxidase polymorphism in pubescent oak (Quercus pubescens Willd.) in relation to Q. petraea Matt. Liebl and Q. robur L.
Publication date: Available online 5 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Maria Krzakowa, Kamilla Bąkowska-Żywicka In spite of the increasing availability of DNA markers with a higher genetic resolution, electrophoretically detectable allozymes still provide the cutting edge in the estimation of genetic variability. Peroxidase was analysed in individual sleeping buds collected in three native species: Quercus pubescens, Q. petraea and Q. robur. The species were characterised with gene and genotype frequencies and showed specific degrees of intra- and inter-population diversity. G...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Optimization of a Photoregeneration System for NADH Using Pristine TiO2 as a Catalyst
In this study, the reduced nicotinamide adenine dinucleotide (NADH) was regenerated with high efficiency via UV photocatalysis using original TiO2 nanoparticles as the catalyst, disodium salt of ethylenediamine tetraacetic acid (EDTA) as the electron donor and [Cp*Rh(bpy)(H2O)]2+ as the mediator. When the loading amount of TiO2 was increased, the initial reaction rate first increased and subsequently remained almost unchanged. In the presence of 1.5mg/mL TiO2, the NADH yield reached approximately 90% after 30min of irradiation. Several electron donors were investigated. Disodium and tetrasodium salt of EDTA were demonstrat...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research

Diacylglycerol production by genetically modified lipase from Malassezia globosa
In this study, a mutant lipase of SMG1 (SMG1-F278D) from Malassezia globosa was studied for the production of DAG. The SMG1-F278D exhibited 4-fold increased esterification activity as well as superior fatty acid (FA) specificity for medium chain FAs. Molecular docking study suggested that the caprylic acid (CA) was strongly bound to the catalytic residue Ser171 present in the SMG1-F278D as compared to SMG1. Molecular Dynamics (MD) simulations were employed in order to understand the structural conformations of SMG1 and SMG1-F278D. The structure of SMG1-F278D was found to be more compact as well as less deviated from its na...
Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research