Catalase immobilized-radiation grafted functional cellulose matrix: A novel biocatalytic

Publication date: Available online 4 January 2017 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Nilanjal Misra, N.K. Goel, S.A. Shelkar, L. Varshney, Virendra Kumar The work highlights a unique, one step protocol for room temperature immobilization of enzyme catalase onto epoxy functionalised cellulose matrix developed via gamma radiation induced simultaneous irradiation grafting of Glycidylmethacrylate (GMA). Effect of grafting parameters, such as radiation dose, monomer concentration and solvent composition on the grafting yield, was studied in order to optimize the radiation grafting process. Poly(GMA)-g-cellulose matrices were characterized by grafting yield determination, FTIR, SEM and TGA techniques. The epoxy functionalized poly(GMA)-g-cellulose matrix was subsequently employed for covalent immobilization of an industrially relevant enzyme catalase. The catalytic activity of catalase-immobilized-poly(GMA)-g-cellulose was assayed by spectrophotometrically monitoring the enzymatic degradation of H2O2 at 240nm. Catalase-immobilized-poly(GMA)-g-cellulose was observed to be reusable for over 5 cycles within ten days without substantial loss in activity. Catalase was observed to show higher activity at higher pH after immobilization. Thermal stability of Catalase-immobilized-poly(GMA)-g-cellulose was also enhanced in comparison to the free enzyme system. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - January 7, 2017 Category: Biochemistry Source Type: research