Journal of Molecular Catalysis B: Enzymatic This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Immobilization of Cellulase onto a Recyclable Thermo-responsive Polymer as Bioconjugate
Publication date: Available online 19 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Zhaoyang Ding, Xuexuan Zheng, Sipeng Li, Xuejun Cao Here we report a recyclable thermo-responsive polymer-cellulase bioconjugate, a recoverable, thermo-responsive polymer (PNMN) which was synthesized through the polymerization of N-isopropylmethacrylamide (NIPMA) with methyl acrylate and N-(Hydroxymethyl) acrylamide was used for connection with cellulase to form bioconjugate. The polymer exhibited a thermo-responsive lower critical solution temperature (LCST). The aminooxy group of PNMN provided a hand...
Source: Journal of Molecular Catalysis B: Enzymatic - March 20, 2016 Category: Biochemistry Source Type: research

Synthesis of acacetin and resveratrol 3,5-di-O-β-glucopyranoside using lipase-catalyzed regioselective deacetylation of polyphenol glycoside peracetates as the key step
Publication date: Available online 19 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Shun Hanamura, Kengo Hanaya, Mitsuru Shoji, Takeshi Sugai Acacetin and resveratrol 3,5-di-O-β-glucopyranoside were synthesized from naturally abundant naringin and piceid in 65% and 62% overall yield, respectively. The key steps were the regioselective deacetylation of the peracetates of the glycosylated forms with Candida antarctica lipase B (Novozym 435) and Burkholderia cepacia lipase (Amano PS-IM). Deacetylation occurred exclusively at the least hindered position of the aromatic moieties and all a...
Source: Journal of Molecular Catalysis B: Enzymatic - March 20, 2016 Category: Biochemistry Source Type: research

Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis
Publication date: Available online 17 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Yuan Liu, Shun-Hua Zhou, Cheng-Yu Rong, Zhe Chi, Zhen-Ming Chi, Guang Lei Liu An exo-inulinase gene from Kluyveromyces marxianus and an endo-inulinase gene from Aspergillus niger were cloned and expressed in Yarrowia lipolytica, respectively. Then, the purified recombinant exo-inulinase (rEXINU) and endo-inulinase (rENINU), either individual or combination with another, were used for hydrolysis of inulin and inulin in tuber meal of Jerusalem artichoke. After the analysis of the dynamic hydrolysis p...
Source: Journal of Molecular Catalysis B: Enzymatic - March 18, 2016 Category: Biochemistry Source Type: research

Reversible immobilization of lipases on octyl-glutamic agarose beads: a mixed adsorption that reinforces enzyme immobilization
Publication date: Available online 16 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Nazzoly Rueda, Cleiton S. dos Santos, Maria Daniela Rodriguez, Tiago L. Albuquerque, Oveimar Barbosa, Rodrigo Torres, Claudia Ortiz, Roberto Fernandez-Lafuente A new octyl-glutamic(OCGLU) heterofunctional agarose bead has been prepared. It has been compared to octyl-agarose (OC) in their performance to immobilize 5 different lipases, those from Candida antarctica (A (CALA) and B (CALB)), from Thermomyces lanuginosus (TLL), from Rhizomucor miehei (RML) and from Candida rugosa (CRL) and a phospho...
Source: Journal of Molecular Catalysis B: Enzymatic - March 17, 2016 Category: Biochemistry Source Type: research

In vivo and In vitro hydroxylation of Cineole and Camphor by Cytochromes P450CYP101A1, CYP101B1 and N242A CYP176A1
In this study three bacterial P450s, P450cam (CYP101A1), CYP101B1 and the mutant N242A-P450cin (N242A-CYP176A1), were used to produce a range of products from the oxidation of the monoterpenes (1R)- and (1S)-camphor and 1,8-cineole. We demonstrate that both in vitro and in vivo catalytic turnover with these P450s can produce a complement of up to seven hydroxycamphors and seven hydroxycineoles, in addition to compounds produced from further oxidation. The CYP101B1 whole cell catalytic system was found to produce 300–600mg/L of culture of oxidation products that could be easily separated chromatographically. The CYP101B1 ...
Source: Journal of Molecular Catalysis B: Enzymatic - March 17, 2016 Category: Biochemistry Source Type: research

Purification and characterization of a glycosidase with hydrolyzing multi-3-O-glycosides of spirostanol saponin activity from Gibberella intermedia
Publication date: Available online 17 March 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Hui Li, Xiangdong Wang, Yang Ma, Nannan Yang, Xiaojuan Zhang, Zhenghong Xu, Jinsong Shi The strain Gibberella intermedia WX12 preserved in our lab showed a strong ability to degrade dioscin from Dioscorea Zingiberensis C. H. Wright into diosgenin. The glycosidase enzyme of this strain was purified by a procedure consisting of ammonium sulfate fractionation, anion-exchange and hydrophobic chromatography and was named GiGly. This enzyme was a monomer with molecular mass of approximately 45kDa. The ...
Source: Journal of Molecular Catalysis B: Enzymatic - March 17, 2016 Category: Biochemistry Source Type: research

Immobilization of Candida Antarctica Lipase B on Epoxy Modified Silica by Sol-Gel Process
Publication date: Available online 26 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Hong-Zhi Ma, Xiao-Wei Yu, Cong Song, Qing-lan Xue, Bo Jiang An epoxy-functionalized silica support was prepared through 3-glycidoxypropyltrimethoxylsilane(KH560)modified silica sol in the presence of triblock copolymer F127 (F-560-S). The surface chemistry, micromorphology and pore structure of the supports were characterized by TG, FTIR, SEM and N2 adsorption-desorption, which showed that the BET surface areas and pore volumes of epoxy-activated supports increased with the addition of F127. The C...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Purple carrots: better biocatalysts for the enantioselective reduction of acetophenones than common orange carrots (D. carota)
Publication date: Available online 26 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Alvaro Takeo Omori, Flávia Gonçalves Lobo, Anna Carolina Gonçalves do Amaral, Camila de Souza de Oliveira The constant and reproducible enantioselective reduction of acetophenones were investigated in several edible plants. Among them, only carrot (Daucus carota) acted as a reliable biocatalyst. Different carrot varieties (orange, yellow and purple carrots) in contact with substituted acetophenones gave the corresponding optically-active alcohols in good-to-excellent rates of conversion with high...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Synthesis of butyl butyrate in batch and continuous enzymatic reactors using Thermomyces lanuginosus lipase immobilized in Immobead 150
Publication date: Available online 26 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Carla R. Matte, Carolina Bordinhão, Jakeline K. Poppe, Rafael C. Rodrigues, Plinho F. Hertz, Marco A.Z. Ayub Thermomyces lanuginosus lipase (TLL) was covalently immobilized on Immobead 150 (ImmTLL) via epoxy groups of the support and the lysine of the enzyme to be used in the synthesis of butyl butyrate. The optimization of the esterification reaction parameters was carried out by central composite design and response surface methodology, having product yield as target, with all tested variabl...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Urease-catalyzed synthesis of aminocyanopyridines from urea under fully green conditions
Publication date: Available online 26 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Fatemeh Tamaddon, Somayeh Ghazi, Mohammad Reza Noorbala This is an original work on catalytic performance of urease in organic synthesis which in one-pot dissociation of urea and condensation of the in situ generated ammonia with aldehydes, acetophenones, and malononitrile occurs in water to give 2-amino-3-cyanopyridines. Comparative experiments with ammonium salts supported the enzymatic specify of 0.01g/mL (50U/mg) of urease for bio-production of ammonia, while trace amount of heavy metal ions such ...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Quantitative insights and improvements of enzyme activity and stereoselectivity for CALB-catalyzed alcoholysis in two-step desymmetrization
Publication date: Available online 27 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Hsu-Ting Hsiao, Shin-Yan Lin, Shau-Wei Tsai A generalized analytical solution for the two-step desymmetrization is recommended for doing the parameter estimation and process simulation in practical applications. A formula is then derived for investigating effects of the dimensionless kinetic parameters and molar fraction of the substrate on varying the enantiomeric excess of the major enantiomer. With the preparation of (R)-3-phenylglutaric 4-(1,2,4-triazolidyl)-butyl ester from 3-phenylglutaric di-1,...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Covalent immobilization of glucose oxidase on mesocellular silica foams: Characterization and stability towards temperature and organic solvents
Publication date: May 2016 Source:Journal of Molecular Catalysis B: Enzymatic, Volume 127 Author(s): N. Balistreri, D. Gaboriau, C. Jolivalt, F. Launay Glucose oxidase (GOx) immobilization onto mesoporous SBA-15 silica and two mesocellular foams (MCF) characterized by similar surface area and pore volumes but different pore/cell dimensions was examined. The covalent grafting of the enzyme through amide bonds was evidenced by controlling pH conditions, thus preventing GOx leaching. The immobilized protein activity was found to be significantly higher for the mesocellular foam with both cells and windows size lar...
Source: Journal of Molecular Catalysis B: Enzymatic - February 29, 2016 Category: Biochemistry Source Type: research

Catalytic properties of a highly thermoactive polygalacturonase from the mesophilic fungus Penicillium occitanis and use in juice clarification
Publication date: Available online 24 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Hajer Tounsi, AzzaHadj Sassi, Zamen Ben Romdhane, Marwa Lajnef, Jean-William Dupuy, Delphine Lapaillerie, Anne-Marie Lomenech, Marc Bonneu, Ali Gargouri, Noomen Hadj-Taieb A new polygalacturonase (PG1) is purified to homogeneity from the hyper-pectinolytic mutant (CT1) of Penicillium occitanis by using two chromatographic steps. The purified PG1 exhibited a very high specific activity towards PGA, namely 57533.2U/mg of protein and a high optimal temperature, 70°C. In our knowledge, it i...
Source: Journal of Molecular Catalysis B: Enzymatic - February 25, 2016 Category: Biochemistry Source Type: research

Behavior of Yarrowia lipolytica Lipase Lip2 under high hydrostatic pressure: Conformational changes and isokineticity diagram
Publication date: Available online 22 February 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Xinying Yang, Gang Chen, Huanmei Du, Ming Miao, Biao Feng Despite the large and increasing interest in Yarrowia lipolytica Lipase Lip2 (Lip2) for biotransformation in food processing, the effect of conformational changes and isokineticity diagram on Lip2’s behaviour still remains unclear. In this paper, the catalytic ability of Lip2 under high hydrostatic pressure was investigated. After treated at 450MPa and 40°C for 10min, the maximum enzyme activity was obtained, which was 209% of that at at...
Source: Journal of Molecular Catalysis B: Enzymatic - February 23, 2016 Category: Biochemistry Source Type: research

Preparation and characterization of sol–gel hybrid coating films for covalent immobilization of lipase enzyme
In this study UV-curable hybrid epoxy-silica polymer films were prepared via sol–gel method. Lipase (EC 3.1.1.3) from Candida rugosa was covalently immobilized onto hybrid epoxy-silica polymer films and immobilization capacity of polymer films was found 7.22mgg−1. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Immobilized and free enzymes were used in two different reaction systems: hydrolysis of p-nitrophenyl palmitate in aqueous medium and synthesis of p-nitrophenyl linoleate (from p-nitrophenol and linoleic acid) in ...
Source: Journal of Molecular Catalysis B: Enzymatic - February 23, 2016 Category: Biochemistry Source Type: research