Off-target effects of cytidine base editor and adenine base editor: what can we do?
Publication date: Available online 2 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Puping Liang, Jinkun Wen, Junjiu Huang (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - November 4, 2019 Category: Genetics & Stem Cells Source Type: research
Acquisition of functional neurons by direct conversion: Switching the developmental clock directly
Publication date: Available online 28 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Shuangquan Chen, Juan Zhang, Dongming Zhang, Jianwei JiaoAbstractIdentifying approaches for treating neurodegeneration is a thorny task but is important for a growing number of patients. Researchers have focused on discovering the underlying molecular mechanisms of reprogramming and optimizing the technologies for acquiring neurons. Direct conversion is one of the most important processes for treating neurological disorders. Induced neurons (iNs) derived from direct conversion, which bypass the pluripotency stage, are more ...
Source: Journal of Genetics and Genomics - October 29, 2019 Category: Genetics & Stem Cells Source Type: research
Rapid breeding of pink-fruited tomato hybrids using CRISPR/Cas9 system
Publication date: Available online 24 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Tianxia Yang, Lei Deng, Wei Zhao, Ruoxi Zhang, Hongling Jiang, Zhibiao Ye, Chang-Bao Li, Chuanyou Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 26, 2019 Category: Genetics & Stem Cells Source Type: research
Establishment and characterization of a complete set of Triticum durum-Thinopyrum elongatum monosomic addition lines with resistance to wheat fusarium head blight
Publication date: Available online 23 October 2019Source: Journal of Genetics and GenomicsAuthor(s): Jing Wang, Qinghua Shi, Xianrui Guo, Fangpu Han (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 23, 2019 Category: Genetics & Stem Cells Source Type: research
A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering
Publication date: Available online 10 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yiman Geng, Hai-Qin Yan, Pei Li, Gai-Xian Ren, Xiao-Peng Guo, Peiqi Yin, Leiliang Zhang, Zhaohui Qian, Zhendong Zhao, Yi-Cheng Sun (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - October 11, 2019 Category: Genetics & Stem Cells Source Type: research
The p.(Pro170Leu) variant in NOG impairs noggin secretion and causes autosomal dominant congenital conductive hearing loss due to stapes ankylosis
Publication date: Available online 27 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yilai Shu, Lijun Wang, Xiaoting Cheng, Chayada Tangshewinsirikul, Weili Shi, Yasheng Yuan, Zhiqiang Yan, Huawei Li, Jun Shen, Bing Chen, Weiguo Zou (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 28, 2019 Category: Genetics & Stem Cells Source Type: research
Identification of transcriptional isoforms associated with survival in cancer patient
In this study we performed the first large-scale identification of transcriptional isoforms that are specifically associated with patient prognosis, even without gene-level association. These specific isoforms are defined as Transcripts Associated with Patient Prognosis (TAPP). Although a group of TAPPs are the principal isoforms of their genes with intact functional protein domains, another group of TAPPs lack important protein domains found in their canonical gene isoforms. This dichotomy in the distribution of protein domains may indicate different patterns of TAPP association with cancer. TAPPs in protein-coding genes,...
Source: Journal of Genetics and Genomics - September 25, 2019 Category: Genetics & Stem Cells Source Type: research
Temperature-sensitive cytoophidium assembly in Schizosaccharomyces pombe
Publication date: Available online 24 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Jing Zhang, Ji-Long LiuAbstractThe metabolic enzyme CTP synthase (CTPS) is able to compartmentalize into filaments, termed cytoophidia, in a variety of organisms including bacteria, budding yeast, fission yeast, fruit flies and mammals. A previous study in budding yeast shows that the filament-forming process of CTPS is not sensitive to temperature shift. Here we study CTPS filamentation in the fission yeast Schizosaccharomyces pombe. To our surprise, we find that both the length and the occurrence of cytoophidia in S. po...
Source: Journal of Genetics and Genomics - September 24, 2019 Category: Genetics & Stem Cells Source Type: research
Examining the practical limits of batch effect-correction algorithms: When should you care about batch effects?
Publication date: Available online 20 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Longjian Zhou, Andrew Chi-Hau Sue, Wilson Wen Bin GohAbstractBatch effects are technical sources of variation and can confound analysis. While many performance ranking exercises have been conducted to establish the best batch effect-correction algorithm (BECA), we hold the viewpoint that the notion of best is context-dependent. Moreover, alternative questions beyond the simplistic notion of “best” are also interesting: are BECAs robust against various degrees of confounding and if so, what is the limit? Using two diff...
Source: Journal of Genetics and Genomics - September 21, 2019 Category: Genetics & Stem Cells Source Type: research
SERCA regulates collective cell migration by maintaining cytoplasmic Ca2+ homeostasis
Publication date: Available online 20 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Xuan Guo, Jun Luo, Heng Wang, Jiong Chen (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 21, 2019 Category: Genetics & Stem Cells Source Type: research
A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering
Publication date: Available online 10 September 2019Source: Journal of Genetics and GenomicsAuthor(s): Yiman Geng, Hai-Qin Yan, Pei Li, Gai-Xian Ren, Xiao-Peng Guo, Peiqi Yin, Leiliang Zhang, Zhaohui Qian, Zhendong Zhao, Yi-Cheng Sun (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - September 10, 2019 Category: Genetics & Stem Cells Source Type: research
Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
In conclusion, these findings will help to further enhance the expansion, persistence and function of anti-CD38 CAR T cells in subsequent clinical trials. (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research
Establishment of wheat-Thinopyrum ponticum translocation lines with resistance to Puccinia graminis f. sp. tritici Ug99
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Hongwei Li, Willem H.P. Boshoff, Zacharias A. Pretorius, Qi Zheng, Bin Li, Zhensheng Li (Source: Journal of Genetics and Genomics)
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research
The expression tractability of biological traits shaped by natural selection
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Li Liu, Jianguo Wang, Jianrong Yang, Feng Wang, Xionglei HeAbstractUnderstanding how gene expression is translated to phenotype is central to modern molecular biology, and the success is contingent on the intrinsic tractability of the specific traits under examination. However, an a priori estimate of trait tractability from the perspective of gene expression is unavailable. Motivated by the concept of entropy in a thermodynamic system, we here propose such an estimate (ST) by gauging the number (N) of expression states that...
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research
OSBPL2-disrupted pigs recapitulate dual features of human hearing loss and hypercholesterolaemia
Publication date: Available online 13 August 2019Source: Journal of Genetics and GenomicsAuthor(s): Jun Yao, Huasha Zeng, Min Zhang, Qinjun Wei, Ying Wang, Haiyuan Yang, Yajie Lu, Rongfeng Li, Qiang Xiong, Lining Zhang, Zhibin Chen, Guangqian Xing, Xin Cao, Yifan DaiAbstractOxysterol binding protein like 2 (OSBPL2), an important regulator in cellular lipid metabolism and transport, was identified as a novel deafness-causative gene in our previous work. To resemble the phenotypic features of OSBPL2 mutation in animal models and elucidate the potential genotype-phenotype associations, the OSBPL2-disrupted Bama miniature (BM)...
Source: Journal of Genetics and Genomics - August 14, 2019 Category: Genetics & Stem Cells Source Type: research
