Bonding of universal adhesive system to enamel surrounding real-life carious cavities
In conclusion, the bond strength to enamel assumed demineralized is lower than to sound enamel. The enamel surrounding carious cavities jeopardize the bonding of universal adhesive system. The bond strength of universal adhesive is similar, regardle ss to bonding strategy. (Source: Brazilian Oral Research)
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Occurrence and predictors of gingivitis and supragingival calculus in a population of Brazilian adults
Abstract: The aim of this study was to determine the occurrence of gingivitis and calculus and their predictors in a population of adults in Brazil. A representative sample of 758 adults from 35 to 59 years of age from Porto Alegre city was examined. A structured questionnaire was applied. The Gingival Bleeding Index and the presence of calculus were measured at 4 sites/tooth. Multivariable logistic models were fitted to determine the predictors for gingival bleeding at>20% of sites. Overall, 96.5% (95% confidence interval [CI]=95.1-98.0) of individuals had ≥ 1 bleeding site. The mean percentages of sites with gingiv...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide
This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric d...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Effect of hydrofluoric acid concentration and etching time on resin-bond strength to different glass ceramics
In conclusion, etching with 5% HF for 20 seconds is recommended for lithium disilicate and leucite-reinforced CAD/CAM ceramics. However, for pressed lithium disilicate ceram ic, 10% HF for 60 s showed significantly higher bond strength to resin cement. (Source: Brazilian Oral Research)
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Dental implant surfaces treated with phosphoric acid can modulate cytokine production by blood MN cells
Abstract: The study characterizes dental implant surfaces treated with phosphoric acid to assess the effects of acid treatment on blood cells and correlate them with cytokine levels. The implant surfaces examined were divided into untreated metal surface (US; n = 50), metal surface treated with phosphoric acid (ATS; n = 50) and cement surface (CS; n = 50) groups. The samples were characterized by scanning electron microscopy (SEM) and rheometry. The implants were incubated with human blood mononuclear cells for 24 h, with surface rinsing in the ATS treatment. Cell viability was determined by colorimetric methods and cytoki...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial
This study was designed as a randomized single-blinded, 2-arm, clinical trial. Twenty-four primarily infected root canals were selected and randomly divided into 2 groups: HyFlex CM (n = 12); and ProTaper Next (n = 12). Samples were collected before and after the biomechanical preparation and inoculated in specific flasks. Irrigation was performed using 2.5% sodium hypochlorite. A kinetic turbidimetric lysate assay of limulus amoebocytes was used to quantify endotoxins. Microbiological culture technique was used to determine the count of bacterial colony forming units (CFU/mL). Data collected were statistically analyzed us...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Effect of iontophoresis on fluoride uptake in enamel with artificial caries lesion
The objective of this research was to evaluate the effect of iontophoresis using different electric current intensities on the uptake of fluoride in dental enamel with artificial caries lesions. In this in vitro operator-blind experiment, bovine enamel blocks (n = 10/group) with caries-like lesions and predetermined surface hardness were randomized into 6 groups: placebo gel without fluoride applied with a current of 0.8 mA (negative control), 2% NaF gel without application of any current, and 2% NaF gel applied with currents of 0.1, 0.2, 0.4 and 0.8 mA. Cathodic iontophoresis was applied for 4 min. The concentration of lo...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Effectiveness of XP-Endo Finisher in the reduction of bacterial load in oval-shaped root canals
In conclusion, XPS and RB files are effective in reducing bacterial levels in oval-shaped RC. The use of XPF as a method of agitation of the irrigating solution improved the cleaning efficiency of both file systems tested. Mechanical preparation performed with saline solution decreased culturable bacteria from the root canal, but antimicrobial substances such as NaOCl should be used to achieve a significantly better disinfection. (Source: Brazilian Oral Research)
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
Correspondence between try-in pastes and resin cements, and color stability of bonded lithium disilicate disks
This study investigates the color correspondence of resin cements and try-in pastes, and the color stability of bonded lithium disilicate ceramic disks. Resin composite disks were fabricated (n = 36) to serve as the background for lithium disilicate disks prepared in two thicknesses (0.5 and 1.0 mm, n = 18 each). Two brands were used for try-in and cement procedures: Variolink Veneer and AllCem Veneer. For baseline, water was applied between the ceramic disks and their respective backgrounds to achieve the control group. This set was subjected to color measurement using an intraoral measurement device (T0). The try-in was ...
Source: Brazilian Oral Research - May 27, 2019 Category: Dentistry Source Type: research
In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells
In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders. (Source: Brazilian Oral Research)
Source: Brazilian Oral Research - April 25, 2019 Category: Dentistry Source Type: research
Adipokine gene variability and plasma levels in patients with chronic periodontitis -a case-control study
Abstract: Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case – control study was to investigate the possible impacts of adiponectin (ADIPOQ), leptin (LEP) and its receptor (LEPR), and resistin (RETN) on the etiopathogenesis of CP. Examinations were performed on 118 non – periodontitis healthy subjects (healthy controls, HC), 205 healthy individuals with CP (H + CP) and 86 type 2 diabetes patients with CP (T2DM + CP). Var...
Source: Brazilian Oral Research - April 25, 2019 Category: Dentistry Source Type: research
Effects of astragaloside IV on inflammation and immunity in rats with experimental periodontitis
In conclusion, AsIV can alleviate inflammation and enhance immunity in rats with experimental periodontitis. (Source: Brazilian Oral Research)
Source: Brazilian Oral Research - April 25, 2019 Category: Dentistry Source Type: research
Surface morphologic evaluation of orthodontic bonding systems under conditions of cariogenic challenge
Abstract: Orthodontic bonding systems are submitted to demineralization and remineralization dynamics that might compromise their surface smoothness, and favor biofilm aggregation and caries development. The aim of the present study was to evaluate the effects of a cariogenic challenge model (in vitro pH-cycling model) on the surface roughness and topography of 3 bonding materials: Transbond ™ XT (XT), Transbond ™ Plus Color Change (PLUS) and Fuji Ortho ™ LC (FUJI), by means of Atomic Force Microscopy (AFM). Six specimens with standardized dimensions and surface smoothness were fabricated per group, and the materi...
Source: Brazilian Oral Research - April 25, 2019 Category: Dentistry Source Type: research
Regulation of ghrelin receptor by microbial and inflammatory signals in human osteoblasts
Abstract: Recently, it has been suggested that the anti-inflammatory hormone ghrelin (GHRL) and its receptor GHS-R may play a pivotal role in periodontal health and diseases. However, their exact regulation and effects in periodontitis are not known. The aim of this in-vitro study was to investigate the effect of microbial and inflammatory insults on the GHS-R1a expression in human osteoblast-like cells. MG-63 cells were exposed to interleukin (IL)-1 β and Fusobacterium nucleatum in the presence and absence of GHRL for up to 2 d. Subsequently, gene expressions of GHS-R1a, inflammatory mediators and matrix metalloproteina...
Source: Brazilian Oral Research - April 25, 2019 Category: Dentistry Source Type: research
Elevated prevalence of high-risk HPV in healthy oral mucosa of users and nonusers of drugs in Northeastern Brazil
Abstract: Variable rates of HPV infection have been reported in healthy oral mucosa worldwide. The main objective of this study was to detect and genotype HPV infection in users and nonusers of drugs with clinically healthy mucosa from the Northeast Brazil. Samples from 105 patients were amplified using the primers MY09/MY11 and GP5+/GP6+, and genotyping was performed by multiplex-PCR for HPV-6/11, 16 and 18. A total of 81.9% samples were positive. Among drug users, 84.5% presented the virus and 20.4% showed multiple infections. Among non-drug users, 78.7% were positive and 13.5% had multiple infections. Limited informatio...
Source: Brazilian Oral Research - April 12, 2019 Category: Dentistry Source Type: research
