3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs
Drug resistance phenotype acquired by the cancer cells is still the major cause of the patient ’s low survival rates. Spheroids in vitro models represent more closely the characteristics of the human tumors, which prompted its use in the development of new therapeutics. In this review, the structural and functional similarities between spheroids and in vivo human solid tumors are reviewed, highlighting the common drug resistance mechanisms, as well as the therapeutic approaches explored to surpass this resistance. AbstractThree ‐dimensional cell culture models, such as spheroids, can be used in the process of the devel...
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Ana S. Nunes,
Andreia S. Barros,
Elisabete C. Costa,
Andr é F. Moreira,
Ilídio J. Correia Tags: REVIEW Source Type: research
Trophoblast –endothelium signaling involves angiogenesis and apoptosis in a dynamic bioprinted placenta model
In this study, we developed a dynamic three‐dimensional (3D) placenta model by bioprinting trophoblasts and an endothelialized lumen in a perfusion bioreactor. We found the 3D printed perfusion bioreactor system significantly augmented responses of endothelia l cells by encouraging network formations and expressions of angiogenic markers, cluster of differentiation 31 (CD31), matrix metalloproteinase‐2 (MMP2), matrix metalloproteinase‐9 (MMP9), and vascular endothelial growth factor A (VEGFA). Bioprinting favored colocalization of trophoblasts with e ndothelial cells, similar to in vivo observations. Additional analy...
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Che ‐Ying Kuo,
Mariya Shevchuk,
Justin Opfermann,
Ting Guo,
Marco Santoro,
John P. Fisher,
Peter CW Kim Tags: ARTICLE Source Type: research
3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Ana S. Nunes,
Andreia S. Barros,
Elisabete C. Costa,
Andr é F. Moreira,
Ilídio J. Correia Source Type: research
Reconstruction of tricarboxylic acid cycle in Corynebacterium glutamicum with a genome ‐scale metabolic network model for
trans‐4‐hydroxyproline production
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Yu Zhang,
Yun Zhang,
Xiuling Shang,
Bo Wang,
Qitiao Hu,
Shuwen Liu,
Tingyi Wen Source Type: research
Trophoblast –endothelium signaling involves angiogenesis and apoptosis in a dynamic bioprinted placenta model
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Che ‐Ying Kuo,
Mariya Shevchuk,
Justin Opfermann,
Ting Guo,
Marco Santoro,
John P. Fisher,
Peter CW Kim Source Type: research
Combinatorial lentiviral gene delivery of pro ‐oligodendrogenic factors for improving myelination of regenerating axons after spinal cord injury
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Dominique R. Smith,
Daniel J. Margul,
Courtney M. Dumont,
Mitchell A. Carlson,
Mary K. Munsell,
Mitchell Johnson,
Brian J. Cummings,
Aileen J. Anderson,
Lonnie D. Shea Source Type: research
Drug ‐delivering nerve conduit improves regeneration in a critical‐sized gap
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Pratima Labroo,
David Hilgart,
Brett Davis,
Christopher Lambert,
Himanshu Sant,
Bruce Gale,
Jill E. Shea,
Jayant Agarwal Source Type: research
The pearl necklace model in protein A chromatography: Molecular mechanisms at the resin interface
Different ratio of antibody to Protein A domain of MabSelect SuRe are found depending on the isotherm region. In the linear range of the isotherme the 1:1 stoichiometry is the most probable configuration. In the non linear range we argue for a 2:1 stoichiometry. According to the stoichiometry antibodies adsorb in different orientations. The 3:1 stoichiometry may be found at higher bulk concentrations. AbstractStaphylococcal protein A chromatography is an established core technology for monoclonal antibody purification and capture in the downstream processing. MabSelect SuRe involves a tetrameric chain of a recombinant form...
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Authors: Goncalo L. Silva,
Jacek Plewka,
Helga Lichtenegger,
Ana C. Dias ‐Cabral,
Alois Jungbauer,
Rupert Tscheließnig Tags: ARTICLE Source Type: research
Cover Image, Volume 115, Number 11, November 2018
The cover image is based on the ArticleContribution of cellulose synthesis, formation of fibrils and their entanglement to the self ‐flocculation of Zymomonas mobilis by Juan Xia et al., DOI:10.1002/bit.26806. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Authors: Juan Xia,
Chen ‐Guang Liu,
Xin‐Qing Zhao,
Yi Xiao,
Xiao‐Xia Xia,
Feng‐Wu Bai Tags: COVER Source Type: research
Biotechnology and Bioengineering: Volume 115, Number 11, November 2018
Biotechnology and Bioengineering, Volume 115, Issue 11, Page 2669-2672, November 2018. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Tags: ISSUE INFORMATION ‐ TOC Source Type: research
The pearl necklace model in protein A chromatography: Molecular mechanisms at the resin interface
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Authors: Goncalo L. Silva,
Jacek Plewka,
Helga Lichtenegger,
Ana C. Dias ‐Cabral,
Alois Jungbauer,
Rupert Tscheließnig Source Type: research
Cover Image, Volume 115, Number 11, November 2018
Biotechnology and Bioengineering,Volume 115, Issue 11, Page i-i, November 2018. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Authors: Juan Xia,
Chen ‐Guang Liu,
Xin‐Qing Zhao,
Yi Xiao,
Xiao‐Xia Xia,
Feng‐Wu Bai Source Type: research
Biotechnology and Bioengineering: Volume 115, Number 11, November 2018
Biotechnology and Bioengineering,Volume 115, Issue 11, Page 2669-2672, November 2018. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 25, 2018 Category: Biomedical Science Source Type: research
Model ‐based identification of cell‐cycle‐dependent metabolism and putative autocrine effects in antibody producing CHO cell culture
In this study, a novel approach combining near‐physiological treatment of cells (including synchronization), population resolved mechanistic modeling and statisti cal analysis was developed to identify population inhomogeneities. Cell‐cycle‐dependent population dynamics and metabolic regulations due to a putative autocrine factor were examined and their impact on the metabolic rates and antibody production of near‐physiologically synchronized CHO DP‐1 2 cell cultures was determined. To achieve this, a population resolved model was extended to describe putative autocrine‐dependentt and cell‐cycle‐related met...
Source: Biotechnology and Bioengineering - October 23, 2018 Category: Biomedical Science Authors: Johannes M öller,
Katrin Korte,
Ralf Pörtner,
An‐Ping Zeng,
Uwe Jandt Tags: ARTICLE Source Type: research
Model ‐based identification of cell‐cycle‐dependent metabolism and putative autocrine effects in antibody producing CHO cell culture
Biotechnology and Bioengineering, EarlyView. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - October 23, 2018 Category: Biomedical Science Authors: Johannes M öller,
Katrin Korte,
Ralf Pörtner,
An‐Ping Zeng,
Uwe Jandt Source Type: research
