An ultra scale ‐down method to investigate monoclonal antibody processing during tangential flow filtration (TFF) using ultrafiltration membranes
This article is protected by copyright. All rights reserved. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 8, 2018 Category: Biomedical Science Authors: Lara Fernandez ‐Cerezo,
Andrea C.M.E Rayat,
Alex Chatel,
Jennifer M. Pollard,
Gary J Lye,
Michael Hoare Tags: ARTICLE Source Type: research
Metabolic Flux Balance Analysis during lactate and glucose concomitant consumption in HEK293 cell cultures
This article is protected by copyright. All rights reserved. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 8, 2018 Category: Biomedical Science Authors: I. Mart ínez‐Monge,
J. Albiol,
M. Lecina,
L. Liste‐Calleja,
J. Miret,
C. Solà,
J. J. Cairó Tags: ARTICLE Source Type: research
An ultra scale ‐down method to investigate monoclonal antibody processing during tangential flow filtration (TFF) using ultrafiltration membranes
This article is protected by copyright. All rights reserved. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 8, 2018 Category: Biomedical Science Authors: Lara Fernandez ‐Cerezo,
Andrea C.M.E Rayat,
Alex Chatel,
Jennifer M. Pollard,
Gary J Lye,
Michael Hoare Tags: ARTICLE Source Type: research
Metabolic Flux Balance Analysis during lactate and glucose concomitant consumption in HEK293 cell cultures
This article is protected by copyright. All rights reserved. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 8, 2018 Category: Biomedical Science Authors: I. Mart ínez‐Monge,
J. Albiol,
M. Lecina,
L. Liste‐Calleja,
J. Miret,
C. Solà,
J. J. Cairó Tags: ARTICLE Source Type: research
Dynamic mass spectrometry probe for electrospray ionization mass spectrometry monitoring of bioreactors for therapeutic cell manufacturing
We present a microfabricated, monolithically integrated device capable of continuous sample collection, treatment, and direct infusion for ESI‐MS detection of biomolec ules in high‐salt solutions. The dynamic mass spectrometry probe (DMSP) uses a microfluidic mass exchanger to rapidly condition samples for online MS analysis by removing interfering salts, while concurrently introducing MS signal enhancers to the sample for sensitive biomolecular detection. Explo iting this active conditioning capability increases MS signal intensity and signal‐to‐noise ratio. As a result, sensitivity for low‐concentration biomole...
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Mason A. Chilmonczyk,
Peter A. Kottke,
Hazel Y. Stevens,
Robert E. Guldberg,
Andrei G. Fedorov Tags: ARTICLE Source Type: research
A microfluidic gradient mixer ‐flow chamber as a new tool to study biofilm development under defined solute gradients
In this study, we report a new biofilm growth system that integrates a microfluidic gradient mixer with a biofilm growth chamber and demonstrate its applicability to study the development of biofilms under defined solute gradients. AbstractUnderstanding the dynamics of biofilm development in response to chemical cues and signals is required toward the development of controllable biofilm ‐mediated bioprocesses. In this study, we report a new biofilm growth system that integrates a microfluidic gradient mixer with a biofilm growth chamber. The biofilm growth system allows biofilms to grow under defined solute gradients and...
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Yingdan Zhang,
Cheng Li,
Yichao Wu,
Yilei Zhang,
Zhi Zhou,
Bin Cao Tags: ARTICLE Source Type: research
Model ‐assisted process characterization and validation for a continuous two‐column protein A capture process
In this study we introduce three process characterization approaches toward validation of continuous twin ‐column capture chromatography (CaptureSMB), referred to as “standard,” “model assisted,” and “hybrid.” They are all based on a traditional risk‐based approach, using process description, risk analysis, design‐of‐experiments (DoE), and statistical analysis as essential elements. AbstractIn this study we introduce three process characterization approaches toward validation of continuous twin ‐column capture chromatography (CaptureSMB), referred to as “standard,” “model assisted,” and “hyb...
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Daniel Baur,
James Angelo,
Srinivas Chollangi,
Thomas M üller‐Späth,
Xuankuo Xu,
Sanchayita Ghose,
Zheng Jian Li,
Massimo Morbidelli Tags: ARTICLE Source Type: research
Dynamic mass spectrometry probe for electrospray ionization mass spectrometry monitoring of bioreactors for therapeutic cell manufacturing
We present a microfabricated, monolithically integrated device capable of continuous sample collection, treatment, and direct infusion for ESI‐MS detection of biomolec ules in high‐salt solutions. The dynamic mass spectrometry probe (DMSP) uses a microfluidic mass exchanger to rapidly condition samples for online MS analysis by removing interfering salts, while concurrently introducing MS signal enhancers to the sample for sensitive biomolecular detection. Explo iting this active conditioning capability increases MS signal intensity and signal‐to‐noise ratio. As a result, sensitivity for low‐concentration biomole...
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Mason A. Chilmonczyk,
Peter A. Kottke,
Hazel Y. Stevens,
Robert E. Guldberg,
Andrei G. Fedorov Tags: ARTICLE Source Type: research
Model ‐assisted process characterization and validation for a continuous two‐column protein A capture process
In this study we introduce three process characterization approaches toward validation of continuous twin ‐column capture chromatography (CaptureSMB), referred to as “standard,” “model assisted,” and “hybrid.” They are all based on a traditional risk‐based approach, using process description, risk analysis, design‐of‐experiments (DoE), and statistical analysis as essential elements. AbstractIn this study we introduce three process characterization approaches toward validation of continuous twin ‐column capture chromatography (CaptureSMB), referred to as “standard,” “model assisted,” and “hyb...
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Daniel Baur,
James Angelo,
Srinivas Chollangi,
Thomas M üller‐Späth,
Xuankuo Xu,
Sanchayita Ghose,
Zheng Jian Li,
Massimo Morbidelli Tags: ARTICLE Source Type: research
On ‐fiber display of a functional peptide at sites distant from the cell surface using a long bacterionanofiber of a trimeric autotransporter adhesin
Biotechnology and Bioengineering,Volume 0, Issue ja, -Not available-. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 6, 2018 Category: Biomedical Science Authors: Hajime Nakatani,
Junichi Kanie,
Katsutoshi Hori Source Type: research
Whole synthetic pathway engineering of recombinant protein production
In this study we describe how these two properties can be simultaneously optimized via design of a product ‐specific combination of synthetic DNA parts to maximize flux through the protein synthetic pathway and the use of a host cell chassis with an increased capability to synthesize both cell and product biomass. AbstractThe output from protein biomanufacturing systems is a function of total host cell biomass synthetic capacity and recombinant protein production per unit cell biomass. In this study, we describe how these two properties can be simultaneously optimized via design of a product ‐specific combination of sy...
Source: Biotechnology and Bioengineering - November 5, 2018 Category: Biomedical Science Authors: Adam J. Brown,
Suzanne J. Gibson,
Diane Hatton,
Claire L. Arnall,
David C. James Tags: ARTICLE Source Type: research
On ‐fiber display of a functional peptide at sites distant from the cell surface using a long bacterionanofiber of a trimeric autotransporter adhesin
This article is protected by copyright. All rights reserved. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - November 5, 2018 Category: Biomedical Science Authors: Hajime Nakatani,
Junichi Kanie,
Katsutoshi Hori Tags: ARTICLE Source Type: research
Drug ‐delivering nerve conduit improves regeneration in a critical‐sized gap
This paper describes the design of a novel drug delivery apparatus integrated with a PTFE (polytetrafluoroethylene) based nerve conduit for controlled local delivery of a protein such as GDNF or a small molecule such as FK506. AbstractAutologous nerve grafts are the current “gold standard” for repairing large nerve gaps. However, they cause morbidity at the donor nerve site and only a limited amount of nerve can be harvested. Nerve conduits are a promising alternative to autografts and can act as guidance cues for the regenerating axons, without the need to harvest donor nerve. Separately, it has been shown that locali...
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Pratima Labroo,
David Hilgart,
Brett Davis,
Christopher Lambert,
Himanshu Sant,
Bruce Gale,
Jill E. Shea,
Jayant Agarwal Tags: ARTICLE Source Type: research
Reconstruction of tricarboxylic acid cycle in Corynebacterium glutamicum with a genome ‐scale metabolic network model for
trans‐4‐hydroxyproline production
The TCA cycle inCorynebacterium glutamicum was reconstructed by inactivating succinyl ‐CoA synthetase to redirect α‐ketoglutarate toward proline hydroxylation. P4H bridged the broken TCA cycle through the conversion of cosubstrate α‐ketoglutarate to succinate and led to a significant increase in Hyp production. Fine‐tuning of ProB* and P4H abundances gave rise to the improv ement in the yield of Hyp on glucose. Abstracttrans‐4‐Hydroxy‐l‐proline (Hyp) is an abundant component of mammalian collagen and functions as a chiral synthon for the syntheses of anti‐inflammatory drugs in the pharmaceutical industr...
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Yu Zhang,
Yun Zhang,
Xiuling Shang,
Bo Wang,
Qitiao Hu,
Shuwen Liu,
Tingyi Wen Tags: ARTICLE Source Type: research
Combinatorial lentiviral gene delivery of pro ‐oligodendrogenic factors for improving myelination of regenerating axons after spinal cord injury
Spinal cord injury (SCI) results in paralysis below the injury and strategies are being developed that support axonal regrowth, yet recovery lags, in part because many axons are not remyelinated. Herein, we investigated strategies to increase myelination of regenerating axons by overexpression of platelet‐derived growth factor‐AA and noggin either alone or in combination in a mouse SCI model. AbstractSpinal cord injury (SCI) results in paralysis below the injury and strategies are being developed that support axonal regrowth, yet recovery lags, in part, because many axons are not remyelinated. Herein, we investigated...
Source: Biotechnology and Bioengineering - October 27, 2018 Category: Biomedical Science Authors: Dominique R. Smith,
Daniel J. Margul,
Courtney M. Dumont,
Mitchell A. Carlson,
Mary K. Munsell,
Mitchell Johnson,
Brian J. Cummings,
Aileen J. Anderson,
Lonnie D. Shea Tags: ARTICLE Source Type: research
