Springer protocols feed by Molecular Medicine 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Care of Rodent Models Used for Preclinical Evaluation of Tissue-Engineered/Regenerative Medicine Product Candidates
We present specific examples of pre-, peri- and post-operative care of rodents using our experiences in developing tissue engineering products for kidney, esophagus, small intestine and lung. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Migration Assay to Evaluate Cellular Interactions with Biomaterials for Tissue Engineering/Regenerative Medicine Applications
Regenerative medicine and tissue engineering approaches for solving current medical dilemmas such as organ failure, congenital defect, or reconstruction following disease or trauma typically require specific considerations regarding biomaterial selection, identification of key cell types, and applicable surgical techniques (Lanza et al. Principles of tissue engineering, Academic, 2007; Kikuchi, Kanama., Quart Rev 24:51–67, 2007). The ability to evaluate these components in vitro under conditions which simulate relevant in vivo environments can reduce development risks including time and money costs associated with ea...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Characterization of a PGA-Based Scaffold for Use in a Tissue-Engineered Neo-Urinary Conduit
A tissue-engineered product needs to be properly characterized in order to be used in vivo. Many methods can be used to characterize a scaffold during creation of a tissue-engineered product. This chapter looks at the mechanical (tensile testing) and biological characterization (cell viability and proliferation) of a polyglycolic acid-based scaffold used to tissue engineer a Neo-Urinary Conduit™. Such methods are more broadly applicable to characterization of other neo-organ product candidates. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Design, Fabrication, and Preparation of Synthetic Scaffolds for Urologic Tissue Engineering
This chapter describes the fabrication of a polyglycolic acid (PGA)-based scaffold used to tissue engineer a Neo-Urinary Conduit™. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Electrospinning Tubular Scaffolds with Tissue-Like Mechanical Properties and Biomimetic Surface Features
Electrospinning is a useful technique for generating scaffolds composed of nano- and/or microfibers. Through an innovative modification of the cylindrical target mandrel it is possible to create macrostructural or microstructural invaginations. The former provides complex bulk mechanical behavior reminiscent of native soft tissue; the latter leads to a surface topography reminiscent of intestinal villi. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Rapid Production of Autologous Fibrin Hydrogels for Cellular Encapsulation in Organ Regeneration
Autologous hydrogel manufacture is an exciting technique within the field of regenerative medicine. Fibrin is a protein with many biocompatible and regenerative features. The ability to generate fibrin scaffolds with the necessary matrix topography for cell integration, from a patient’s autologous tissue, could improve the translation of many tissue engineering efforts from bench to clinical application. Here we describe the rapid extraction and production of fibrin hydrogels for development of organs, using a simple low-cost series of centrifugations and ethanol precipitation, which produces hydrogels of a more pred...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Preparation and Evaluation of Natural Scaffold Materials for Kidney Regenerative Applications
Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and integration. This biomaterial manufacturing technique is simple, cost-effective, highly reproducible and the in vivo evaluation procedure highly informative on the biocompatibility and regenerative potential of the tested construct. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine
The following chapter outlines methodologies to phenotypically characterize primary cells for the use in tissue-engineered and regenerative medicine applications. Methods covered include analyzing cells using immunocytochemistry, fluorescence-activated cell sorting, and confocal microscopy of adherent and suspended cells, as well as combinations of formulated cell–biomaterial constructs. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Three-Dimensional Visualization of the Molecular Architecture of Cell–Cell Junctions In Situ by Cryo-Electron Tomography of Vitreous Sections
Cryo-electron tomography of vitreous sections is currently the only method for visualizing the eukaryotic ultrastructure at close to native state with molecular resolution. Here, we describe the detailed procedure of how to prepare suitable vitreous sections from mammalian skin for cryo-electron tomography, how to align the projection images of the tilt-series, and finally how to perform sub-tomogram averaging on macromolecular complexes with periodic arrangement such as desmosomes. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
Molecular Diagnosis of Genodermatoses
The progress of molecular genetics helps clinicians to prove or exclude a suspected diagnosis for a vast and yet increasing number of genodermatoses. This leads to precise genetic counselling, prenatal diagnosis and preimplantation genetic haplotyping for many inherited skin conditions. It is also helpful in such occasions as phenocopy, late onset and incomplete penetrance, uniparental disomy, mitochondrial inheritance and pigmentary mosaicism. Molecular methods of two genodermatoses are explained in detail, i.e. genodermatoses with skin fragility and neurofibromatosis type 1. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
RNA Trans-Splicing for Genodermatoses
Spliceosome-mediated RNA trans-splicing (SMaRT) is a tool that facilitates the recombination of two distinct pre-mRNA molecules. Its application for gene therapeutic purposes has been hindered by laborious procedures to identify gene-specific molecules. We have established a screening method for the identification of highly functional RNA trans-splicing molecules based on fluorescence reporters, facilitating the generation of most potent therapeutic molecules for the correction of any gene of interest. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
Molecular Diagnosis of Autoimmune Blistering Diseases
Autoimmune bullous diseases are the best-characterized autoimmune skin diseases. Molecular diagnosis of these diseases has become possible due to the identification of their target autoantigens over the past three decades. In this review, we summarize methodology for categorizing autoimmune bullous diseases by means of combinations of direct and indirect immunofluorescence techniques using normal human skin sections, rat bladder sections and COS7 cells transfected with desmocollins 1–3 encoded vectors, enzyme-linked immunosorbent assays and immunoblotting with normal human epidermal extracts, dermal extracts, purifie...
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
Cell- and Protein-Based Therapy Approaches for Epidermolysis Bullosa
Dystrophic epidermolysis bullosa (DEB) is a clinically heterogeneous heritable skin fragility disorder characterized by mechanically induced mucocutaneous blistering. On the molecular level DEB is caused by mutations leading to deficiency in collagen VII (CVII), a large extracellular protein building anchoring fibrils that attach the epidermis to the dermis. Severely affected patients suffer from wounds, which heal with excessive scarring causing mutilating deformities of hands and feet. The patients are also predisposed to development of aggressive squamous cell carcinomas at sites of chronic wounds. Currently no availabl...
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
Zebrafish as a Model System to Study Heritable Skin Diseases
Heritable skin diseases represent a broad spectrum of clinical manifestations due to mutations in ∼500 different genes. A number of model systems have been developed to advance our understanding of the pathomechanisms of genodermatoses. Zebrafish (Danio rerio), a freshwater vertebrate, has a well-characterized genome, the expression of which can be easily manipulated. The larvae develop rapidly, with all major organs having largely developed by 5–6 days post-fertilization, including the skin which consists at that stage of the epidermis comprising two cell layers and separated from the dermal collagenous matrix b...
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils, and Macrophages
Tissue macrophages and inflammatory neutrophils represent important cells of the innate immune system responsible for various important tasks, i.e., elimination of pathogens and/or granuloma formation. Isolation of large numbers of primary phagocytes is vital for research with these cells. Within this protocol, we present a strategy for isolation of large numbers of inflammatory neutrophils and macrophages from murine skin that allows for follow-up in vivo or in vitro studies. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - December 19, 2012 Category: Molecular Biology Source Type: news
