Springer protocols feed by Molecular Medicine 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Isolation of Urothelial Cells from Bladder Tissue
Presented below is a methodology for the isolation, expansion, and maintenance of urothelial cells derived from human bladder. Such bladder-derived urothelial cells, taken together with bladder or alternately sourced smooth muscle cells, may be complexed with an appropriately shaped biodegradable scaffold to create regenerative constructs capable of seeding formation of new bladder or bladder-like neo-organs upon implantation in human cystectomy patients. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Isolation of Smooth Muscle Cells from Bladder for Generation of Engineered Urologic Organs
The isolation of smooth muscle cells from bladder tissue is a valuable technique used in cell biology research and tissue engineering. Smooth muscle cells can be used for analysis in many areas including, but not limited to, cell function and genotype experimentation. Smooth muscle cells can also be used in tissue engineering applications for research and/or regenerative medicine. Replacement tissue or tissue for augmentation can be created to stem or remediate problems in the urologic system. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Histological Evaluation of Tissue Regeneration Using Biodegradable Scaffold Seeded by Autologous Cells for Tubular/Hollow Organ Applications
The accurate interpretation of histological outcomes is a critical endpoint in preclinical studies. Thus, the toxicologic pathologist plays a vital role in conducting a comprehensive microscopic evaluation that would ultimately help defining the safety and functionality in Tissue Engineering/Regenerative Medicinal (TERM) products. In spite of many advances in regenerative medicine, there are no specific guidelines for the histological assessment of TERM products (Jayo et al. Toxicol Pathol 36:92–96, 2008). In this chapter, we describe the methodology designed to facilitate the detection of structural and functional c...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Genotypic and Phenotypic Analysis of In Vivo Tissue Regeneration in an Animal Model
Determining the in vivo response to cellular therapies is important in evaluating the effectiveness of regenerative medicine therapies. Such treatment modalities leverage the treated individual’s ability to elicit the body’s innate healing response to repair/regenerate damaged tissues or organs. Detailed within this chapter is the process of evaluating the host tissue response to a candidate cell therapy through analysis of key transcript and protein targets. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Scanning Electron Microscopy Evaluation of Endothelialized Tissue-Engineered Constructs
Scanning electron microscopy (SEM) is an important technique for evaluation of the efficiency of endothelialization of tissue-engineered constructs incorporating a surface endothelial cell layer. Here, we describe methodologies for the preparation of such constructs for SEM analysis that are applicable to a broad range of tissue-engineered constructs. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Tissue Engineering of Esophagus and Small Intestine in Rodent Injury Models
Regenerative constructs composed of synthetically sourced, biodegradable biomaterials seeded with smooth muscle-like cells have been leveraged to mediate regeneration of bladder and bladder-like neo-organs. Here, we describe how such constructs may be applied to catalyze regeneration of esophagus and small intestine in preclinical rodent models. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
A “Living Bioreactor” for the Production of Tissue-Engineered Small Intestine
Here, we describe the use of a mouse model as a living bioreactor for the generation of tissue-engineered small intestine. Small intestine is harvested from donor mice with subsequent isolation of organoid units (a cluster of mesenchymal and epithelial cells). Some of these organoid units contain pluripotent stem cells with a preserved relationship with the mesenchymal stem cell niche. A preparation of organoid units is seeded onto a biodegradable scaffold and implanted intraperitoneally within the omentum of the host animal. The cells are nourished initially via imbibition until neovascularization occurs. This technique a...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold
As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor. (Source: Springer protocols feed by Molecular Medicine)
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Formulation of Selected Renal Cells for Implantation into a Kidney
Delivery of cells to organs has primarily relied on formulating the cells in a nonviscous liquid carrier. We have developed a methodology to isolate selected renal cells (SRC) that have provided functional stability to damaged kidneys in preclinical models (Kelley et al. Poster presentation at
71st scientific sessions of American diabetes association
, 2011; Kelley et al. Oral presentation given at Tissue Engineering and Regenerative Medicine International Society (TERMIS)—North America annual conference, 2010; Presnell et al. Tissue Eng Part C Methods 17:261–273,...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Bioengineered Skin Substitutes
Bioengineered skin has great potential for use in regenerative medicine for treatment of severe wounds such as burns or chronic ulcers. Genetically modified skin substitutes have also been used as cell-based devices or “live bioreactors” to deliver therapeutics locally or systemically. Finally, these tissue constructs are used as realistic models of human skin for toxicological testing, to speed drug development and replace traditional animal-based tests in a variety of industries. Here we describe a method of generating bioengineered skin based on a natural scaffold, namely, decellularized human dermis and epi...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Microencapsulation of Pancreatic Islets for Use in a Bioartificial Pancreas
Islet transplantation is the most exciting treatment option for individuals afflicted with Type 1 diabetes. However, the severe shortage of human pancreas and the need to use risky immunosuppressive drugs to prevent transplant rejection remain two major obstacles for the routine use of islet transplantation in diabetic patients. Successful development of a bioartificial pancreas using the approach of microencapsulation with perm-selective coating of islets with biopolymers for graft immunoisolation holds tremendous promise for diabetic patients because it has great potential to overcome these two barriers. In this chapter,...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Isolation of Human Cadaveric Pancreatic Islets for Clinical Transplantation
Diabetes is a debilitating condition which can lead to chronic vascular, renal, and ophthalmic disease. Type I or Juvenile Diabetes is caused by the destruction of beta cells within the islets of Langerhans within the pancreas. The beta cells are able to maintain tight control of blood glucose levels by virtue of their ability to secrete insulin in response to small increases in blood glucose concentration. In the absence of beta cells patients with Type I diabetes are dependent on the exogenous administration of insulin. This results in imperfect control of blood glucose levels. In early animal and human studies, it was s...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering
Human adipose-derived adult stem cells (ASCs) represent a unique population of multipotent stem cells. Their utility in a variety of tissue engineering applications, and as a model system for the study of molecular mechanisms of differentiation, is well established. In addition, their relative abundance, ease of isolation from human subcutaneous lipoaspirates, and functional stability make them an excellent physiologically relevant platform. Here, we describe detailed procedures for handling and purification of ASCs from lipoaspirate, as well as their expansion, cryopreservation, quality control, and functional assays. (So...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Construction of a Multicoaxial Hollow Fiber Bioreactor
Bioreactors are assembled tools conceived to exploit engineering principles with inbuilt biological relevance. Such reactors are created as in vitro models to better replicate natural in vivo organs. These biotools are subsets within the interdisciplinary tissue engineering field and are established as inert devices to improve upon biological stimuli while simultaneously allowing tissue functional properties to be nondestructively measured. Design and fabrication efforts are focused on two-dimensional (2D) and three-dimensional (3D) physical constructs while linking environment–cell relations, the microenvironme...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
Bioreactor Design Considerations for Hollow Organs
There are many important considerations in the design, construction, and use of a bioreactor for growing hollow organs such as vessels, gastrointestinal tissue, esophagus, and others. The growth of new organs requires a specialized container that provides sterility and an environment conducive to cell-seeding and attachment onto a three-dimensional bioabsorbable porous scaffold, incubation, maturation, and shipping for implantation. The materials’ selection, dimensions, manufacturing, testing, and use of the bioreactor are all factors that should be considered in designing a bioreactor for the development of hollow o...
Source: Springer protocols feed by Molecular Medicine - April 11, 2013 Category: Molecular Biology Source Type: news
