Springer protocols feed by Imaging/Radiology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.High-Pressure Fluorescence Applications
Fluorescence is the most widely used technique to study the effect of pressure on biochemical systems. The use of pressure as a physical variable sheds light into volumetric characteristics of reactions. Here we focus on the effect of pressure on protein solutions using a simple unfolding example in order to illustrate the applications of the methodology. Topics covered in this review include the relationships between practical aspects and technical limitations; the effect of pressure and the study of protein cavities; the interpretation of thermodynamic and relaxation kinetics; and the study of relaxation amplitudes. Fina...
Source: Springer protocols feed by Imaging/Radiology - October 14, 2013 Category: Radiology Source Type: news
Quantitative Fluorescence Spectral Analysis of Protein Denaturation
This chapter describes a procedure of global analysis of the steady-state spectra measured with different concentrations of the denaturant to quantitatively study protein denaturation. With the help of physicochemical models, relevant spectral parameters that characterize the folding intermediate and thermodynamic parameters that describe a three-state model N
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Source: Springer protocols feed by Imaging/Radiology - October 14, 2013 Category: Radiology Source Type: news
Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein–ligand complex. (Source: Springer protocols feed by Imaging/Radiology)
Source: Springer protocols feed by Imaging/Radiology - October 14, 2013 Category: Radiology Source Type: news
How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
Contemporary spectrofluorimeters comprise exciting light sources, excitation and emission monochromators, and detectors that without correction yield data not conforming to an ideal spectral response. The correction of the spectral properties of the exciting and emission light paths first requires calibration of the wavelength and spectral accuracy. The exciting beam path can be corrected up to the sample position using a spectrally corrected reference detection system. The corrected reference response accounts for both the spectral intensity and drift of the exciting light source relative to emission and/or transmission d...
Source: Springer protocols feed by Imaging/Radiology - October 14, 2013 Category: Radiology Source Type: news
