Celebrating the 25th birthday of intracytoplasmic sperm injection
(Source: Reproduction)
Source: Reproduction - December 15, 2017 Category: Reproduction Medicine Authors: O'Neill, C. L., Palermo, G. D. Tags: Editorial Source Type: research
Spatio-temporal localization of S100P during the peri-implantation period in the mouse
(Source: Reproduction)
Source: Reproduction - December 7, 2017 Category: Reproduction Medicine Authors: Zhang, R., Wang, L., Xia, H., Zhang, W. Tags: Retraction Source Type: research
Intracytoplasmic sperm injection: state of the art in humans
Among infertile couples, 25% involve both male and female factors, while male factor alone accounts for another 25% due to oligo-, astheno-, teratozoospermia, a combination of the three, or even a complete absence of sperm cells in the ejaculate and can lead to a poor prognosis even with the help of assisted reproductive technology (ART). Intracytoplasmic sperm injection (ICSI) has been with us now for a quarter of a century and in spite of the controversy generated since its inception, it remains in the forefront of the techniques utilized in ART. The development of ICSI in 1992 has drastically decreased the impact of mal...
Source: Reproduction - December 6, 2017 Category: Reproduction Medicine Authors: Palermo, G. D., ONeill, C. L., Chow, S., Cheung, S., Parrella, A., Pereira, N., Rosenwaks, Z. Tags: Anniversary Review Source Type: research
Stem cells, in vitro gametogenesis and male fertility
Reconstitution in culture of biological processes, such as differentiation and organization, is a key challenge in regenerative medicine, and one in which stem cell technology plays a central role. Pluripotent stem cells and spermatogonial stem cells are useful materials for reconstitution of germ cell development in vitro, as they are capable of differentiating into gametes. Reconstitution of germ cell development, termed in vitro gametogenesis, will provide an experimental platform for a better understanding of germ cell development, as well as an alternative source of gametes for reproduction, with the potential to cure...
Source: Reproduction - November 28, 2017 Category: Reproduction Medicine Authors: Nagamatsu, G., Hayashi, K. Tags: Anniversary Review Source Type: research
The pioneering of intracytoplasmic sperm injection: historical perspectives
Intracytoplasmic sperm injection (ICSI) has often been heralded as a ground-breaking technique that has transformed the treatment of couples with infertility. By injecting a single spermatozoon into the cytoplasm of the oocyte, ICSI bypasses the zona pellucida and increases the chances of fertilization and subsequent embryo development, independent of semen parameters. Ever since the first live births using ICSI were reported in 1992, ICSI has become the mainstay of treating male factor infertility as well as overcoming fertilization failure associated with conventional in vitro insemination. Today, ICSI is utilized in nea...
Source: Reproduction - November 22, 2017 Category: Reproduction Medicine Authors: Rosenwaks, Z., Pereira, N. Tags: Anniversary Review Source Type: research
Clusterin in the mouse epididymis: possible roles in sperm maturation and capacitation
Clusterin (CLU) is known as an extracellular chaperone for proteins under stress, thus preventing them from aggregation and precipitation. We showed herein that CLU, expressed by principal cells of the mouse caput epididymis, was present in high amounts in the lumen. In the cauda epididymis, CLU bound tightly to the sperm head surface and its amount on total sperm was similar to that in the bathing luminal fluid. In both immotile and motile caudal epididymal sperm, CLU was localized over the entire sperm head except at the convex ridge, although in the motile sperm population, the CLU immunofluorescence pattern was distinc...
Source: Reproduction - November 8, 2017 Category: Reproduction Medicine Authors: Saewu, A., Kadunganattil, S., Raghupathy, R., Kongmanas, K., Astudillo, P. D., Hermo, L., Tanphaichitr, N. Tags: Research Source Type: research
In vitro maturation impacts cumulus-oocyte complex metabolism and stress in cattle
The influence of in vitro maturation (IVM) in oocytes is still not totally understood. The aim of this study was to determine the influence of IVM on the metabolism and homeostasis of bovine cumulus-oocyte complexes. In the present study, we demonstrated that IVM leads to accumulation of neutral lipids associated with differential levels of the mono-, di- and triacylglycerols in both cumulus cells and oocytes. We observed that in vitro-matured oocytes exhibited decreased glutathione and reactive oxygen species levels and a lower ATP/ADP ratio when compared to in vivo-matured oocytes, with no significant differences in meta...
Source: Reproduction - November 3, 2017 Category: Reproduction Medicine Authors: del Collado, M., da Silveira, J. C., Oliveira, M. L. F., Alves, B. M. S. M., Simas, R. C., Godoy, A. T., Coelho, M. B., Marques, L. A., Carriero, M. M., Nogueira, M. F. G., Eberlin, M. N., Silva, L. A., Meirelles, F. V., Perecin, F. Tags: Research Source Type: research
Subcellular localization of MS4A13 isoform 2 in mouse spermatozoa
We report that the MS4A family member, MS4A13-2, may form oligomers in sperm membranes, which may be involved in an interaction with the zona pellucida or cumulus during fertilization. (Source: Reproduction)
Source: Reproduction - November 3, 2017 Category: Reproduction Medicine Authors: Kaneko, T., Toshimori, K., Iida, H. Tags: Research Source Type: research
Dyslipidemia alters sperm maturation and capacitation in LXR-null mice
This study is the first showing an impact of dyslipidemia on post-testicular sperm maturation with consequences on the capacitation signaling cascade. It may lead to the identification of fertility prognostic markers in this pathophysiological situation, which could help clinicians to better understand male infertilities which are thus far classified as idiopathic. (Source: Reproduction)
Source: Reproduction - November 3, 2017 Category: Reproduction Medicine Authors: Whitfield, M., Guiton, R., Rispal, J., Acar, N., Kocer, A., Drevet, J. R., Saez, F. Tags: Research Source Type: research
GPR62 constitutively activates cAMP signaling but is dispensable for male fertility in mice
In this study, we found that Gpr62 is also expressed in male germ cells in mice, and its expression increases along with sperm differentiation. GPR62 lacks the BBXXB and DRY motifs, which are conserved across many GPCRs, and it was able to induce cAMP signaling in the absence of a ligand. These structural and functional features are conserved among mammals, and the mutant analysis of GPR62 has revealed that lacking of these motifs is involved in the constitutive activity. We also found that GPR62 can homooligomerize, but it is not sufficient for its constitutive activity. We further investigated its physiological function ...
Source: Reproduction - November 3, 2017 Category: Reproduction Medicine Authors: Muroi, T., Matsushima, Y., Kanamori, R., Inoue, H., Fujii, W., Yogo, K. Tags: Research Source Type: research
Production of cloned mice using oocytes derived from ICR-outbred strain
Recently, it has become possible to generate cloned mice using a somatic cell nucleus derived from not only F1 strains but also inbred strains. However, to date, all cloned mice have been generated using F1 mouse oocytes as the recipient cytoplasm. Here, we attempted to generate cloned mice from oocytes derived from the ICR-outbred mouse strain. Cumulus cell nuclei derived from BDF1 and ICR mouse strains were injected into enucleated oocytes of both strains to create four groups. Subsequently, the quality and developmental potential of the cloned embryos were examined. ICR oocytes were more susceptible to damage associated...
Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Tanabe, Y., Kuwayama, H., Wakayama, S., Nagatomo, H., Ooga, M., Kamimura, S., Kishigami, S., Wakayama, T. Tags: Research Source Type: research
The crosstalk between endometrial stromal cells and macrophages impairs cytotoxicity of NK cells in endometriosis by secreting IL-10 and TGF-{beta}
The dysfunction of NK cells in women with endometriosis (EMS) contributes to the immune escape of menstrual endometrial fragments refluxed into the peritoneal cavity. The reciprocal communications between endometrial stromal cells (ESCs) and lymphocytes facilitate the development of EMS. However, the mechanism of these communications on cytotoxicity of natural killer (NK) cells in endometriotic milieus is still largely unknown. To imitate the local immune microenvironment, the co-culture systems of ESCs from patients with EMS and monocyte-derived macrophages or of ESCs, macrophages and NK cells were constructed. The cytoki...
Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Yang, H.-L., Zhou, W.-J., Chang, K.-K., Mei, J., Huang, L.-Q., Wang, M.-Y., Meng, Y., Ha, S.-Y., Li, D.-J., Li, M.-Q. Tags: Research Source Type: research
Embryo cell allocation patterns are not altered by biopsy but can be linked with further development
In conclusion, these results suggest that single blastomere removal does not disturb cell allocation patterns during pre-implantation. Nonetheless, the results suggest that embryos following different cell allocation patterns present different coping mechanisms against in vitro manipulations and further development might be altered. (Source: Reproduction)
Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Sepulveda-Rincon, L. P., Islam, N., Marsters, P., Campbell, B. K., Beaujean, N., Maalouf, W. E. Tags: Research Source Type: research
Altered miRNA-profile dependent on ART outcome in early pregnancy targets Wnt-pathway
Main goal of this study is to detect the possible alterations in microRNA (miRNA) expression and the pathway targeted in plasma at the time of embryo transfer and pregnancy testing dependent on the assisted reproductive treatment (ART) outcome after ovarian hyperstimulation for in vitro fertilization. Changes in miRNA expression in plasma of women, who became pregnant (n = 6) vs women who failed implantation (n = 6) following day 5 embryo transfer (ET), were investigated at the day of ET and pregnancy testing (PT). Protein expression to validate the finding was performed with a sample size of n = 20 (10 per group) using en...
Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Freis, A., Keller, A., Ludwig, N., Meese, E., Jauckus, J., Rehnitz, J., Capp, E., Strowitzki, T., Germeyer, A. Tags: Research Source Type: research
Profiling of miRNAs in porcine germ cells during spermatogenesis
In this study, we purified porcine spermatogonia, pachytene spermatocytes and round spermatids using a STA-PUT apparatus. A small RNA deep sequencing and analysis were conducted to establish a miRNAs profiling in these male germ cells. We found that 19 miRNAs were differentially expressed between spermatogonia and pachytene spermatocytes, and 74 miRNAs differentially expressed between pachytene spermatocytes and round spermatids. Furthermore, 91 miRNAs were upregulated, while 108 miRNAs were downregulated in spermatozoa. We demonstrated that ssc-miR-10a-5p, ssc-miR-125b, ssc-let-7f and ssc-miR-186 were highly expressed in ...
Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Chen, X., Che, D., Zhang, P., Li, X., Yuan, Q., Liu, T., Guo, J., Feng, T., Wu, L., Liao, M., He, Z., Zeng, W. Tags: Research Source Type: research
