Profiling of miRNAs in porcine germ cells during spermatogenesis

In this study, we purified porcine spermatogonia, pachytene spermatocytes and round spermatids using a STA-PUT apparatus. A small RNA deep sequencing and analysis were conducted to establish a miRNAs profiling in these male germ cells. We found that 19 miRNAs were differentially expressed between spermatogonia and pachytene spermatocytes, and 74 miRNAs differentially expressed between pachytene spermatocytes and round spermatids. Furthermore, 91 miRNAs were upregulated, while 108 miRNAs were downregulated in spermatozoa. We demonstrated that ssc-miR-10a-5p, ssc-miR-125b, ssc-let-7f and ssc-miR-186 were highly expressed in spermatogonia, pachytene spermatocytes, round spermatids and spermatozoa respectively. The findings could provide novel insights into roles of miRNAs in regulation of porcine spermatogenesis.

Source: Reproduction - October 31, 2017 Category: Reproduction Medicine Authors: Chen, X., Che, D., Zhang, P., Li, X., Yuan, Q., Liu, T., Guo, J., Feng, T., Wu, L., Liao, M., He, Z., Zeng, W. Tags: Research Source Type: research