Lactoferrin increases sperm membrane functionality of frozen equine semen
Contents
During cryopreservation, sperm was submitted to an increase in reactive oxygen species generation. This work aimed to improve the quality of frozen equine sperm after the addition of antioxidants lactoferrin (Lf) and catalase (Cat) to a freezing extender. Semen from six stallions was frozen with the extenders: F1) control, INRA 82 freezing extender, F2) F1 + 500 μg/ml Lf and F3) F1 + 200 IU/ml Cat. After thawing, sperm motility parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome‐reacted sperm were evaluated with a computer‐assisted sperm analysis, a hypoosmot...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: HS Martins, GC da Silva, SF Cortes, FO Paes, OA Martins Filho, MSS Araujo, R Stahlberg, MA Lagares Tags: ORIGINAL ARTICLE Source Type: research
The localization and function of p38 α mitogen‐activated protein kinase in rat oocytes
In this study, we investigated the expression, subcellular localization and functional roles of p38α MAPK during the meiotic maturation of rat oocytes. We found that p38α MAPK phosphorylation (p‐p38α MAPK, indicative of p38α MAPK activation) was low at the germinal vesicle (GV) stage, increased 3 hr after germinal vesicle breakdown (GVBD) and maintained its maximum at metaphase I (MI) or metaphase II (MII). The p‐p38α MAPK mainly accumulated in the GV and had no obvious expression in the nucleus. From GVBD to MII, p‐p38α MAPK was distributed in the cytoplasm around either the chromosomes or the spindle. We use...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: S Hu, Q Yu, Y Wang, D Ke, F Zhou, G Cheng, W Xia, C Zhu Tags: ORIGINAL ARTICLE Source Type: research
Characterization of anti ‐Müllerian hormone in a case of bovine male pseudohermaphroditism
This study is the first to show the characterization of AMH in a male pseudohermaphrodite calf. AMH levels in peripheral blood might be useful to diagnose male pseudohermaphroditism in cattle. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: G Kitahara, H El ‐Sheikh Ali, APP Teh, Y Hidaka, S Haneda, S Mido, R Yamaguchi, T Osawa Tags: SHORT COMMUNICATION Source Type: research
Effect of body condition score and reuse of progesterone ‐releasing intravaginal devices on conception rate following timed artificial insemination in Nelore cows
This study had the aim of investigating the efficiency of timed artificial insemination (TAI) through the progesterone‐releasing intravaginal device (PRID), used in new condition and for the second and third times in Nelore cows. The effects of device reuse and body condition score (BCS) on the conception rate (CR) were evaluated in 1,122 multiparous Nelore cows (mean BCS of 2.7 ± 0.4), which were randomly distributed into three groups that received new (n = 330), once (n = 439) and twice used (n = 353) PRID. Among the 1,122 females that underwent TAI, 573 became pregnant, thus representing an overall CR of 51.0...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: LL Pereira, AP Ferreira, WG Vale, LR Serique, KAL Neves, AC Morini, BM Monteiro, AHH Minervino Tags: ORIGINAL ARTICLE Source Type: research
Profiling of miRNAs in neonatal cloned bovines with collapsed lungs and respiratory distress
This study aimed to examine differentially expressed miRNAs in collapsed lungs of cloned bovine neonates and normal lungs in order to identify key pathways and functions that might be related to the pathogenesis of neonatal respiratory distress. In this study, miRNA transcriptomes of collapsed lungs of neonatal cloned bovines and normal lungs were analysed by next‐generation sequencing and the results were validated using quantitative real‐time PCR (qRT‐PCR). A total of 177 differentially expressed miRNAs were identified in the two groups (fold change > 2, RPM ≥ 5), some of which were associated with type II...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: FY Zhang, N Yang, YF Rao, WH Du, HS Hao, XM Zhao, HB Zhu, Y Liu Tags: ORIGINAL ARTICLE Source Type: research
Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm
In conclusion, elephant sperm motility was affected by a dilution effect and restoration of SP proportion with stallion SP, but not with elephant SP, could improve motility in chilled highly diluted sperm. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: A Pinyopummin, S Mahasawangkul, G Nunklang, K Kornkaewrat, S Laopiem, S Koonjaenak, P Wattananit Tags: ORIGINAL ARTICLE Source Type: research
Short ‐term tolerance of equine spermatozoa to various abiotic factors
This study analyses the effect on normospermic samples, but future research could look at the tolerance that asthenozoospermic equine samples have to these abiotic influences. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: CC P érez‐Marín, FD Requena, A Arando, L Requena, F Requena, EI Agüera Tags: ORIGINAL ARTICLE Source Type: research
Effects of the supplementation with an high ‐polyphenols extra‐virgin olive oil on kinetic sperm features and seminal plasma oxidative status in healthy dogs
In conclusion, our results highlight the positive effects of EVOO polyphenols on sperm PROGR% in healthy dogs. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - January 31, 2018 Category: Reproduction Medicine Authors: V Tufarelli, A Rizzo, GM Lacalandra, AC Guaricci, V Laudadio, L Valentini Tags: ORIGINAL ARTICLE Source Type: research
Effects of the supplementation with an high ‐polyphenols extra‐virgin olive oil on kinetic sperm features and seminal plasma oxidative status in healthy dogs
Reproduction in Domestic Animals, EarlyView. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - January 30, 2018 Category: Reproduction Medicine Source Type: research
Effects of two freezing methods and two cryopreservation media on post ‐thaw quality of stallion spermatozoa
Reproduction in Domestic Animals,Volume 53, Issue 2, Page 519-524, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - January 30, 2018 Category: Reproduction Medicine Source Type: research
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Reproduction in Domestic Animals, Ahead of Print. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - January 30, 2018 Category: Reproduction Medicine Source Type: research
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Reproduction in Domestic Animals,Volume 53, Issue 2, Page 519-524, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - January 30, 2018 Category: Reproduction Medicine Source Type: research
Effects of two freezing methods and two cryopreservation media on post ‐thaw quality of stallion spermatozoa
The objective of this experiment was to compare the post‐thaw characteristics of the same ejaculates cryopreserved in a Styrofoam box or in an automatic freezer, utilizing a glycerol‐based extender (Gent) and an extender that combines methylformamide and glycerol (BotuCrio®). For that, one ejaculate from 30 stallions collected in two different centres was used. For data analysis, a mixed linear model with laboratory, medium and freezing method and respective interactions as fixed effects was used. Stallion was taken into account as a random effect. There was no influence (p > .05) of laboratory, while stallion ef...
Source: Reproduction in Domestic Animals - January 30, 2018 Category: Reproduction Medicine Authors: S Macedo, M Bliebernicht, J Carvalheira, A Costa, F Ribeiro, A Rocha Tags: ORIGINAL ARTICLE Source Type: research
Comparison of efficiency of in vitro cloned sheep embryo production by conventional somatic cell nuclear transfer and handmade cloning technique
Contents
Conventional somatic cell nuclear transfer (SCNT) technique of in vitro production of cloned embryos involves use of costly and complicated micromanipulators. Handmade cloning (HMC) technique has been applied as efficient and cost‐effective alternative in many livestock species. The aim of the present study was to compare the efficiency of in vitro production and in vitro development of cloned sheep embryos by the two techniques. Cloned embryos were produced by conventional SCNT using micromanipulator apparatus and by HMC technique. Enucleation efficiency and efficiency of fusion with somatic cell (nucleus donor...
Source: Reproduction in Domestic Animals - January 23, 2018 Category: Reproduction Medicine Authors: S Khan, M Tali, A Khan, S Bhat, A Ashraf, MH Bhat, F Khan, RA Shah Tags: ORIGINAL ARTICLE Source Type: research
Monitoring changes in back fat thickness and its effect on the restoration of ovarian activity and fertility in Bos indicus cows
Contents
With the objective of testing the hypothesis if animals with a stable layer of body fat (FAT) during the peripartum have a better chance of becoming pregnant after calving, fifty‐nine multiparous Brahman cows in their last trimester of pregnancy were used. Animals averaged four parturitions and were stocked at a rate of 1.25 animal units per hectare and divided into two groups depending on the time postpartum (dpp) that the intravaginal releasing device CIDR was inserted; Group 1 (<30 dpp; n = 30) received the implant at 25.2 ± 4.21 and withdrawn 9 days later. Group 2 (≥30 dpp; n = 29) received th...
Source: Reproduction in Domestic Animals - January 22, 2018 Category: Reproduction Medicine Authors: BR D íaz, CS Galina, I Rubio, M Corro, JL Pablos, A Orihuela Tags: ORIGINAL ARTICLE Source Type: research
