Functional proteomic analysis of crossbred (Holstein Friesian  × Sahiwal) bull spermatozoa
Reproduction in Domestic Animals, EarlyView. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 7, 2018 Category: Reproduction Medicine Source Type: research

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Reproduction in Domestic Animals, Ahead of Print. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 7, 2018 Category: Reproduction Medicine Source Type: research

Functional proteomic analysis of crossbred (Holstein Friesian  × Sahiwal) bull spermatozoa
Contents Male infertility is one of the prime concerns of dairy cattle production. The study was designed to find out differentially expressed proteins in categorized crossbred (Holstein Friesian × Sahiwal) bull semen to serve as potential biomarkers for male infertility. Frozen crossbred bull semen with satisfactory phenotypic records were defined as “good” and “poor” based on their fertility rates. A total of 1,547 proteins were detected in bull spermatozoa using liquid chromatography–mass spectrometer (LC‐MS/MS) analysis. Results revealed that 558 (36.1%) and 653 (42.2%) proteins were expressed to good an...
Source: Reproduction in Domestic Animals - February 7, 2018 Category: Reproduction Medicine Authors: R Singh, GS Sengar, U Singh, R Deb, V Junghare, S Hazra, S Kumar, S Tyagi, AK Das, TV Raja, A Kumar Tags: ORIGINAL ARTICLE Source Type: research

Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian elephant sperm
Reproduction in Domestic Animals,Volume 53, Issue 2, Page 525-533, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 6, 2018 Category: Reproduction Medicine Source Type: research

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Reproduction in Domestic Animals,Volume 53, Issue 2, Page 525-533, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 6, 2018 Category: Reproduction Medicine Source Type: research

Specific fetometric formulas of ICC and BP for calculating the parturition date in the miniature breeds of canine
Reproduction in Domestic Animals,Volume 53, Issue 2, Page 545-549, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 5, 2018 Category: Reproduction Medicine Source Type: research

Short ‐term tolerance of equine spermatozoa to various abiotic factors
Reproduction in Domestic Animals,Volume 53, Issue 2, Page 534-544, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 5, 2018 Category: Reproduction Medicine Source Type: research

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Reproduction in Domestic Animals,Volume 53, Issue 2, Page 545-549, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 5, 2018 Category: Reproduction Medicine Source Type: research

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Reproduction in Domestic Animals,Volume 53, Issue 2, Page 534-544, April 2018. (Source: Reproduction in Domestic Animals)
Source: Reproduction in Domestic Animals - February 5, 2018 Category: Reproduction Medicine Source Type: research

Specific fetometric formulas of ICC and BP for calculating the parturition date in the miniature breeds of canine
In this study, foetal biometry parameters were obtained using serial ultrasonographic examinations in 24 pregnant bitches of six different miniature breeds (≤5 kg). The mating time was unknown. Overall, 25 inner chorionic cavity diameter (ICC) and 22 biparietal diameter (BP) measurements were taken. The relationship between ICC or BP growth and days to parturition was analysed by linear regression. The results showed a significant relationship between days before delivery and ICC or BP. The equations derived from the growth curves can be used in practical prediction of parturition date in miniature dog breeds. (Source: ...
Source: Reproduction in Domestic Animals - February 5, 2018 Category: Reproduction Medicine Authors: P Socha, T Janowski Tags: ORIGINAL ARTICLE Source Type: research

Immunolocalization of angiogenic growth factors in the ovine uterus during the oestrus cycle and in response to Steroids
Contents The vascular changes associated with endometrial maturation in preparation for embryo implantation depend on numerous growth factors, known to regulate key angiogenic events. Primarily, the vascular endothelial growth factor (VEGF) family promotes vascular growth, whilst the angiopoietins maintain blood vessel integrity. The aim was to analyse protein levels of VEGFA ligand and receptors, Angiopoietin‐1 and 2 (ANG1/2) and endothelial cell receptor tyrosine kinase (TIE‐2) in the ovine endometrium in the follicular and luteal phases of the oestrus cycle and in response to ovarian steroids. VEGFA and its receptor...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: TD Tremaine, AA Fouladi ‐Nashta Tags: ORIGINAL ARTICLE Source Type: research

Blastocyst production after intracytoplasmic sperm injection with semen from a stallion with testicular degeneration
Contents In horse breeding, intracytoplasmic sperm injection (ICSI) has gained interest to obtain offspring from subfertile individuals. This paper presents a case report of a stallion with severe testicular degeneration. Semen analysis showed very low motility and 83.5% of detached heads. Histology of a testicular biopsy showed severely decreased spermatogenesis, while transmission electron microscopy of the sperm cells revealed no significant abnormalities. A total of 39 oocytes were fertilized by ICSI with frozen‐thawed spermatozoa of this stallion: 25 oocytes with intact spermatozoa and 24 with detached heads. When u...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: K Roels, K Smits, C Ververs, J Govaere, K D'Herde, A Van Soom Tags: SHORT COMMUNICATION Source Type: research

Follicular fluid stimulates capacitation and acrosome reaction in alpaca sperm (Vicugna pacos)
In this study, the effect of alpaca follicular fluid (aFF) on the motility and acrosome reaction of alpaca spermatozoa was observed, using follicular fluid of three follicle sizes: small (<3 mm), medium (3‐6 mm) and large (>6 mm), in a concentration of 30%. Sperm motility at the first hour of incubation with aFF of small follicles was 48.0%, with aFF of medium follicles it was 43.33% and with aFF of large follicles, it was 34.53%, while control averaged 26.00%. At the second hour, control achieved an average of 28.13%, treatment with aFF from small follicles showed an average of 46.53%, with aFF from medium foll...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: Z Bravo, M Valdivia Tags: ORIGINAL ARTICLE Source Type: research

Vaginal isolation of beta ‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters
Contents The aim of the study was to identify beta‐haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty‐one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta‐haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: AE Guerrero, MC Stornelli, SB Jurado, G Giacoboni, GH Sguazza, RL Sota, MA Stornelli Tags: ORIGINAL ARTICLE Source Type: research

Histological and immunohistochemical evaluation of granulosa cells during different stages of folliculogenesis in bovine ovaries
In this study, 10 morphologically normal bovine ovaries were collected to study the structural aspects of different stages of GC using intermediate filament protein antibodies including cytokeratin AE1/AE3 (AE1/AE3), vimentin, nectin‐4 and desmin. Hormonal immunolocalization was assessed using the immunomarkers anti‐Müllerian hormone (AMH) and inhibin alpha. In addition, tumour markers and proliferation markers using c‐erbB‐2 oncoprotein and proliferating cell nuclear antigen, respectively, were investigated. The immunolabelling of AE1/AE3 in GC was strongest in the early follicle stage and gradually decreased whe...
Source: Reproduction in Domestic Animals - February 1, 2018 Category: Reproduction Medicine Authors: APP Teh, UZ Izzati, K Mori, N Fuke, T Hirai, G Kitahara, R Yamaguchi Tags: ORIGINAL ARTICLE Source Type: research