Molecular and Biochemical Parasitology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Purification of native Sigma class glutathione transferase from Fasciola hepatica
Publication date: June 2018Source: Molecular and Biochemical Parasitology, Volume 222Author(s): Joshua Duncan, David Cutress, Russell M. Morphew, Peter M. BrophyAbstractFascioliasis is a parasitic disease of grazing livestock and a threat to global food security by significantly reducing the production value of sheep, goats and cattle. Moreover, the zoonotic parasite is also a re-emerging food borne threat to human populations. Driven by climate change, the prevalence of fascioliasis is set to increase. Efforts to control the main causative agent, Fasciola hepatica, are hampered by short lived chemotherapy approaches that ...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

A conserved human DJ1-subfamily motif (DJSM) is critical for anti-oxidative and deglycase activities of Plasmodium falciparum DJ1
Publication date: June 2018Source: Molecular and Biochemical Parasitology, Volume 222Author(s): Divya N. Nair, Rajesh Prasad, Neha Singhal, Manish Bhattacharjee, Renu Sudhakar, Pushpa Singh, Subramonian Thanumalayan, Uday Kiran, Yogendra Sharma, Puran Singh SijwaliAbstractPlasmodium falciparum DJ1 (PfDJ1) belongs to the DJ-1/ThiJ/PfpI superfamily whose members are present in all the kingdoms of life and exhibit diverse cellular functions and biochemical activities. The common feature of the superfamily is the class I glutamine amidotransferase domain with a conserved redox-active cysteine residue, which mediates various ac...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Circulating cell-free DNA in patients with alveolar echinococcosis
This study sought to investigate the presence of ccfDNA in patients with AE, and its potential usefulness for the evaluation of treatment efficiency. To achieve these aims, a quantitative PCR and a droplet digital PCR were developed to detect E. multilocularis ccfDNA. An AE animal model identified, for the first time, the presence of large quantities of ccfDNA. Samples from patients with AE (n = 31) were then analyzed twice, at diagnosis, and after three months of chemotherapy: about 25% were positive, almost always with very low concentrations of ccfDNA. These results confirmed that E. multilocularis produces ccfDNA, ...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Revealing genetic hybridization and DNA recombination of Fasciola hepatica and Fasciola gigantica in nuclear introns of the hybrid Fasciola flukes
Publication date: Available online 28 June 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Weerachai Saijuntha, Chairat Tantrawatpan, Takeshi Agatsuma, Chennan Wang, Pewpan M. Intapan, Wanchai Maleewong, Trevor N. PetneyAbstractFasciola hepatica and F. gigantica are ruminant liver flukes that are found worldwide. They can occur sympatrically and hybridize, consequently producing an intermediate form (Fasciola sp.), especially in Africa and Asia. The bridge intron (TkBridgeInt) and intron 4 of domain 2 (TkD2Int4) of the taurocyamine kinase gene were cloned and sequenced from 14 F. hepatica, 18 F. gigantica, and...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Leishmania donovani PP2C: kinetics, structural attributes and in vitro immune response
Publication date: Available online 28 June 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Jakkula Pranay, Rahila Qureshi, Atif Iqbal, S.R. Sagurthi, Insaf A. QureshiAbstractMost of the signaling pathways are regulated by the reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. The current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Leishmania donovani PP2C was cloned into expression vector followed by purification using Ni-NTA affinity chromatograp...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Characterization of a Plasmodium falciparum rRNA methyltransferase
Publication date: July 2018Source: Molecular and Biochemical Parasitology, Volume 223Author(s): Kirti Gupta, Ankit Gupta, Saman HabibAbstractThe ribosomal RNA adenine dimethyltransferases (rAD) of KsgA/Dim1 family are universally conserved with eukaryotic rADs separated into distinct cytosolic Dim1 and organellar KsgA/TFB homologs. Among the two putative KsgA proteins encoded by the Plasmodium falciparum genome, we found that PfKsgA1 was dually localised in the cytoplasm and the mitochondrion. The protein interacted specifically with small ribosomal subunit as detected by ribosome pull-down using anti-PfKsgA1 antibodies. R...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Modulation of cholesterol-related sterols during Eimeria bovis macromeront formation and impact of selected oxysterols on parasite development
Publication date: July 2018Source: Molecular and Biochemical Parasitology, Volume 223Author(s): A. Taubert, L.M.R. Silva, Z.D. Velásquez, C. Larrazabal, D. Lütjohann, C. HermosillaAbstractObligate intracellular apicomplexan parasites are considered as deficient in cholesterol biosynthesis and scavenge cholesterol from their host cell in a parasite-specific manner. Compared to fast proliferating apicomplexan species producing low numbers of merozoites per host cell, (e. g. Toxoplasma gondii), the macromeront-forming protozoa Eimeria bovis is in extraordinary need for cholesterol for offspring production (≥ 170,000 meroz...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Conditional knock-down of a novel coccidian protein leads to the formation of aberrant apical organelles and abrogates mature rhoptry positioning in Toxoplasma gondii
Publication date: July 2018Source: Molecular and Biochemical Parasitology, Volume 223Author(s): Juliette Morlon-Guyot, Laurence Berry, Isabelle Sauquet, Gurman Singh Pall, Hiba El Hajj, Markus Meissner, Wassim DaherAbstractToxoplasma gondii which is a member of the coccidian parasites owns a spatially polarized secretory system, which synthesizes de novo micronemes and rhoptries. These apical secretory organelles discharge their contents into host cells promoting invasion and survival. Herein, we identified a novel Coccidian Specific CORVET/HOPS Associated Protein (CSCHAP) belonging to the interaction network of both tethe...
Source: Molecular and Biochemical Parasitology - July 5, 2018 Category: Parasitology Source Type: research

Revealing genetic hybridization and DNA recombination of Fasciola hepatica and Fasciola gigantica in nuclear introns of the hybrid Fasciola flukes
Publication date: Available online 28 June 2018 Source:Molecular and Biochemical Parasitology Author(s): Weerachai Saijuntha, Chairat Tantrawatpan, Takeshi Agatsuma, Chennan Wang, Pewpan M. Intapan, Wanchai Maleewong, Trevor N. Petney Fasciola hepatica and F. gigantica are ruminant liver flukes that are found worldwide. They can occur sympatrically and hybridize, consequently producing an intermediate form (Fasciola sp.), especially in Africa and Asia. The bridge intron (TkBridgeInt) and intron 4 of domain 2 (TkD2Int4) of the taurocyamine kinase gene were cloned and sequenced from 14 F. hepatica, 18 F. gigantica, ...
Source: Molecular and Biochemical Parasitology - June 29, 2018 Category: Parasitology Source Type: research

Leishmania donovani PP2C: kinetics, structural attributes and in vitro immune response
Publication date: Available online 28 June 2018 Source:Molecular and Biochemical Parasitology Author(s): Jakkula Pranay, Rahila Qureshi, Atif Iqbal, S.R. Sagurthi, Insaf A. Qureshi Most of the signaling pathways are regulated by the reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. The current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Leishmania donovani PP2C was cloned into expression vector followed by purification using Ni-NTA affinity chromatogra...
Source: Molecular and Biochemical Parasitology - June 29, 2018 Category: Parasitology Source Type: research

Conditional knock-down of a novel coccidian protein leads to the formation of aberrant apical organelles and abrogates mature rhoptry positioning in Toxoplasma gondii
Publication date: Available online 27 June 2018 Source:Molecular and Biochemical Parasitology Author(s): Juliette Morlon-Guyot, Laurence Berry, Isabelle Sauquet, Gurman Singh Pall, Hiba El Hajj, Markus Meissner, Wassim Daher Toxoplasma gondii which is a member of the coccidian parasites owns a spatially polarized secretory system, which synthesizes de novo micronemes and rhoptries. These apical secretory organelles discharge their contents into host cells promoting invasion and survival. Herein, we identified a novel Coccidian Specific CORVET/HOPS Associated Protein (CSCHAP) belonging to the interaction network of...
Source: Molecular and Biochemical Parasitology - June 28, 2018 Category: Parasitology Source Type: research

Characterization of a Plasmodium falciparum rRNA methyltransferase
Publication date: July 2018 Source:Molecular and Biochemical Parasitology, Volume 223 Author(s): Kirti Gupta, Ankit Gupta, Saman Habib The ribosomal RNA adenine dimethyltransferases (rAD) of KsgA/Dim1 family are universally conserved with eukaryotic rADs separated into distinct cytosolic Dim1 and organellar KsgA/TFB homologs. Among the two putative KsgA proteins encoded by the Plasmodium falciparum genome, we found that PfKsgA1 was dually localised in the cytoplasm and the mitochondrion. The protein interacted specifically with small ribosomal subunit as detected by ribosome pull-down using anti-PfKsgA1 antibodies. Re...
Source: Molecular and Biochemical Parasitology - June 21, 2018 Category: Parasitology Source Type: research

Modulation of cholesterol-related sterols during Eimeria bovis macromeront formation and impact of selected oxysterols on parasite development
Publication date: Available online 15 June 2018 Source:Molecular and Biochemical Parasitology Author(s): A. Taubert, L.M.R. Silva, Z.D. Velásquez, C. Larrazabal, D. Lütjohann, C. Hermosilla Obligate intracellular apicomplexan parasites are considered as deficient in cholesterol biosynthesis and scavenge cholesterol from their host cell in a parasite-specific manner. Compared to fast proliferating apicomplexan species producing low numbers of merozoites per host cell, (e. g. Toxoplasma gondii), the macromeront-forming protozoa Eimeria bovis is in extraordinary need for cholesterol for offspring production (≥ 170...
Source: Molecular and Biochemical Parasitology - June 15, 2018 Category: Parasitology Source Type: research

Purification of native Sigma Class Glutathione Transferase from Fasciola hepatica
Publication date: Available online 27 April 2018 Source:Molecular and Biochemical Parasitology Author(s): Joshua Duncan, David Cutress, Russell M. Morphew, Peter M. Brophy Fascioliasis is a parasitic disease of grazing livestock and a threat to global food security by significantly reducing the production value of sheep, goats and cattle. Moreover, the zoonotic parasite is also a re-emerging food borne threat to human populations. Driven by climate change, the prevalence of fascioliasis is set to increase. Efforts to control the main causative agent, Fasciola hepatica, are hampered by short lived chemotherapy approac...
Source: Molecular and Biochemical Parasitology - May 25, 2018 Category: Parasitology Source Type: research

A conserved human DJ1-subfamily motif (DJSM) is critical for anti-oxidative and deglycase activities of Plasmodium falciparum DJ1
Publication date: Available online 10 May 2018 Source:Molecular and Biochemical Parasitology Author(s): Divya N. Nair, Rajesh Prasad, Neha Singhal, Manish Bhattacharjee, Renu Sudhakar, Pushpa Singh, Subramonian Thanumalayan, Uday Kiran, Yogendra Sharma, Puran Singh Sijwali Plasmodium falciparum DJ1 (PfDJ1) belongs to the DJ-1/ThiJ/PfpI superfamily whose members are present in all the kingdoms of life and exhibit diverse cellular functions and biochemical activities. The common feature of the superfamily is the class I glutamine amidotransferase domain with a conserved redox-active cysteine residue, which mediat...
Source: Molecular and Biochemical Parasitology - May 25, 2018 Category: Parasitology Source Type: research