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Dysregulated gene expression in oocysts of Plasmodium berghei LAP mutants
Publication date: Available online 10 February 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Sadia Saeed, Ching In Lau, Annie Z. Tremp, Tessa Crompton, Johannes T. DessensAbstractMalaria parasite oocysts generate sporozoites by a process termed sporogony. Essential for successful sporogony of Plasmodium berghei in Anopheles stephensi mosquitoes is a complex of six LCCL lectin domain adhesive-like proteins (LAPs). LAP null mutant oocysts undergo growth and mitosis but fail to form sporozoites. At a cytological level, LAP null mutant oocyst development is indistinguishable from its wildtype counterparts for th...
Source: Molecular and Biochemical Parasitology - February 10, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: March 2019Source: Molecular and Biochemical Parasitology, Volume 228Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - February 9, 2019 Category: Parasitology Source Type: research

Characterization of proteolytic activities of Giardia lamblia with the ability to cleave His-tagged N-terminal sequences
Publication date: Available online 15 January 2019Source: Molecular and Biochemical ParasitologyAuthor(s): José Ignacio de la Mora-de la Mora, Sergio Enríquez-Flores, Cynthia Fernández-Lainez, Pedro Gutiérrez-Castrellón, Alfonso Olivos-García, Augusto González-Canto, Roberto Hernández, Hugo D. Luján, Itzhel García-Torres, Gabriel López-VelázquezAbstractGiardia lamblia is one of the most common protozoan infectious agents in the world and is responsible for diarrheal disease and chronic postinfectious illness. During the host-parasite interaction, proteases are important molecules related to virulence, invasion,...
Source: Molecular and Biochemical Parasitology - January 16, 2019 Category: Parasitology Source Type: research

Stable transfection of Eimeria necatrix through nucleofection of second generation merozoites
Publication date: Available online 15 January 2019Source: Molecular and Biochemical ParasitologyAuthor(s): Chunhui Duan, Dandan Hu, Xinming Tang, Jingxia Suo, Si wang, Sixin Zhang, Geru Tao, Chao Li, Chaoyue Wang, Xiaolong Gu, Xiaoli Tang, Guangping Huang, Biqi Xiang, Shaoqiang Wu, Choukri Ben Mamoun, Xun Suo, Xianyong LiuAbstractEimeria spp., the causative agents of coccidiosis, are the most common protozoan pathogens of chickens. Infection with these parasites can result in poor development or death of animals leading to a devastating economic impact on poultry production. The establishment of transfection protocols for ...
Source: Molecular and Biochemical Parasitology - January 15, 2019 Category: Parasitology Source Type: research

Transcriptomic analysis of reduced sensitivity to praziquantel in Schistosoma mansoni
In this study, we examine the transcriptome of three generations of a laboratory strain of S. mansoni (PR1) whose susceptibility to PZQ has been diminished across 9 passages through exposure to increasing sub-lethal doses of the drug. Miracidial susceptibility was significantly reduced after exposure to 2 x 50 mg/Kg PZQ during the first passage. Susceptibility of worms in vivo was first assessed during passage 5 when mice infected with PZQ-selected schistosomes were dosed with a lethal dose of 3 x 300 mg/kg PZQ resulting in only a 10% reduction in worm number compared to control treatment. The emergence of redu...
Source: Molecular and Biochemical Parasitology - January 15, 2019 Category: Parasitology Source Type: research

Editorial Board
Publication date: January 2019Source: Molecular and Biochemical Parasitology, Volume 227Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - January 6, 2019 Category: Parasitology Source Type: research

Impact of transposable elements in the architecture of genes of the human parasite Schistosoma mansoni
Publication date: Available online 29 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Gisele S. Philippsen, Ricardo DeMarcoAbstractThe parasites belonging to the genus Schistosoma are agents of schistosomiasis, a disease estimated as affecting 235 million people in the world. To better understand the structure of Schistosoma mansoni genome, transposable elements (TEs) distribution and impact on gene structures were investigated. Our analyses indicated a differential distribution of TEs throughout the gene structure. Introns located at the 5' end of the genes are less prone to display TEs and introns l...
Source: Molecular and Biochemical Parasitology - December 29, 2018 Category: Parasitology Source Type: research

A novel 6-pyrophosphorylating IP6 kinase (IP6-6K) discovered in the protozoon Trichomonas vaginalis
Publication date: Available online 26 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Torsten Wundenberg, Marcus M. Nalaskowski, Benjamin Löser, Werner Fanick, Thomas Hackl, Ursula Fürnkranz, Christoph Rehbach, Hongying Lin, Georg W. MayrAbstractThe parasitic protozoon Trichomonas vaginalis is the pathogen of trichomoniasis, the most common non-viral, sexually transmitted disease in humans. Inositol phosphates function in the pathomechanisms of a number of human pathogenic protozoa. Recent findings point to a role of inositol phosphates in T. vaginalis’ adaption to oxygen exposure during change of...
Source: Molecular and Biochemical Parasitology - December 27, 2018 Category: Parasitology Source Type: research

Protean Permeases: Diverse Roles for Membrane Transport Proteins in Kinetoplastid Protozoa
This article highlights the diversity of activities that these integral membrane proteins serve and underscores the emerging complexity of their functions. (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - December 26, 2018 Category: Parasitology Source Type: research

The Crystal Structure of Glucokinase from Leishmania braziliensis
Publication date: Available online 17 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Gregory S. Buechner, Matthew E. Millington, Kay Perry, Edward L. D’AntonioAbstractGlucokinase from pathogenic protozoa of the genus Leishmania is a potential drug target for the chemotherapeutic treatment against leishmaniasis because this enzyme is located at a nodal point between two critically important metabolic pathways, glycolysis and the pentose phosphate pathway (PPP). L. braziliensis glucokinase (LbGlcK) was evaluated for its structural characterization and enzymatic performance. The enzyme catalyzes the p...
Source: Molecular and Biochemical Parasitology - December 18, 2018 Category: Parasitology Source Type: research

Cellular landmarks of Trypanosoma brucei and Leishmania mexicana
Publication date: Available online 11 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Clare Halliday, Karen Billington, Ziyin Wang, Samuel Dean, Jack Daniel Sunter, Richard John WheelerAbstractThe kinetoplastids Trypanosoma brucei and Leishmania mexicana are eukaryotes with a highly structured cellular organisation that is reproduced with great fidelity in each generation. The pattern of signal from a fluorescently tagged protein can define the specific structure/organelle that this protein localises to, and can be extremely informative in phenotype analysis in experimental perturbations, life cycle t...
Source: Molecular and Biochemical Parasitology - December 12, 2018 Category: Parasitology Source Type: research

Genome aware CRISPR gRNA target prediction for parasitic nematodes
Publication date: Available online 7 December 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Damien M. O’HalloranAbstractThe pace of research towards a genetic model to understand the unique molecular biology of parasitic nematodes has increased recently. This research has developed a diverse suite of genetic tools for a variety of parasitic nematodes. CRISPR/Cas9 technology in particular offers much promise as a game changing tool for researchers studying parasitic nematodes. Unlike RNAi, which depends on diverse nematode effectors to silence gene expression, the effectors for CRISPR/Cas9 mutations are typ...
Source: Molecular and Biochemical Parasitology - December 7, 2018 Category: Parasitology Source Type: research

Shared expression of mucin12 in Ascaris lumbricoides and the human small intestine
This study focuses on the host-parasite relationship of human Ascaris lumbricoides, which is a parasite of the small intestine and is also one of the commonest parasites worldwide. As part of this investigation, we examined the host-parasite relationship assuming that there is a common antigenicity, shared protein between A. lumbricoides and human small intestinal mucosa, using molecular techniques. We obtained three DNA clones from human colon cDNA library by screening for anti-A. lumbricoides polyclonal antibodies. The transmembrane mucin12 gene was identified after sequencing analysis of these clones. Specific signals o...
Source: Molecular and Biochemical Parasitology - November 25, 2018 Category: Parasitology Source Type: research

Membrane protein carbonylation of Plasmodium falciparum infected erythrocytes under conditions of sickle cell trait and G6PD deficiency
Publication date: Available online 22 November 2018Source: Molecular and Biochemical ParasitologyAuthor(s): Neyder Contreras Puentes, Erika Rodríguez Cavallo, Darío Méndez CuadroAbstractDeficiency of glucose-6-phosphate dehydrogenase (G6PD) and sickle cell trait (SCT) are described as the polymorphic disorders prevalent in erythrocytes. Both are considered the result of the selective pressure exerted by Plasmodium parasites over human genome, due to a certain degree of resistance to the clinical symptoms of severe malaria. There exist in both a prooxidant environment that favors the oxidative damage on membrane proteins...
Source: Molecular and Biochemical Parasitology - November 23, 2018 Category: Parasitology Source Type: research

Editorial Board
Publication date: December 2018Source: Molecular and Biochemical Parasitology, Volume 226Author(s): (Source: Molecular and Biochemical Parasitology)
Source: Molecular and Biochemical Parasitology - November 19, 2018 Category: Parasitology Source Type: research