Journal of Cellular and Molecular Medicine 
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.α‐galactosylceramide generates lung regulatory T cells through the activated natural killer T cells in mice
AbstractOur previous study showed that intraperitoneal injection of α‐galactosylceramide (α‐GalCer) has the ability to activate lung iNKT cells, but α‐GalCer‐activated iNKT cells do not result in airway inflammation in wild‐type (WT) mice. Many studies showed that iNKT cells had the capacity to induce Treg cells, which gave rise to peripheral tolerance. Therefore, we examined the influence of intraperitoneal administration of α‐GalCer on the expansion and suppressive activity of lung Treg cells using iNKT cell‐knockout mice and co‐culture experiments in vitro. We also compared airway inflammation and a...
Source: Journal of Cellular and Molecular Medicine - November 13, 2018 Category: Molecular Biology Authors: Qianhui Chen,
Xuxue Guo,
Nishan Deng,
Linlin Liu,
Shuo Chen,
Ailing Wang,
Ruiyun Li,
Yi Huang,
Xuhong Ding,
Hongying Yu,
Suping Hu,
Hanxiang Nie Tags: ORIGINAL ARTICLE Source Type: research
Betulinic acid induces apoptosis and suppresses metastasis in hepatocellular carcinoma cell lines in vitro and in vivo
In this study, we determined the effects of BA on proliferation, apoptosis, invasion, and metastasis in HCC cell lines and on tumour growth and pulmonary metastasis in mice. The results suggested that BA could inhibit cell viability and prol iferation of HCC cell lines including HepG2, LM3, and MHCC97H. In addition, BA induced apoptosis of HepG2 cells characterised condensed nuclei and nuclear fragmentation. Moreover, western blot analysis showed that BA‐induced apoptosis associated with increasing of pro‐apoptotic protein Bax and c leaved caspase‐3 and decreasing of anti‐apoptotic protein Bcl‐2. Meanwhile, BA al...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Wei Wang,
Yang Wang,
Mingxing Liu,
Yifan Zhang,
Tao Yang,
Dongsheng Li,
Yinpeng Huang,
Qing Li,
Guang Bai,
Linlin Shi Tags: ORIGINAL ARTICLE Source Type: research
Zbed3 promotes proliferation and invasion of lung cancer partly through regulating the function of Axin ‐Gsk3β complex
AbstractOur previous work showed that Zbed3 is overexpressed in nonsmall cell lung cancer and that down ‐regulation of Zbed3 inhibited β‐catenin expression and cancer cell proliferation and invasiveness. Here, we investigated Zbed3's ability to promote lung cancer cell proliferation and invasion and the involvement of the Axin/TPC/glycogen synthase kinase 3β (Gsk‐3β) complex to the response. Coimmunoprecipitation assays showed that wild‐type Zbed3 bound to Axin but a Zbed3 mutant lacking the Axin binding site did not. In A549 and H1299 lung cancer cells, Zbed3 overexpression promoted cancer cell proliferation an...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Xiuying Shi,
Yang Zhao,
Chuifeng Fan Tags: ORIGINAL ARTICLE Source Type: research
Combined treatment of sodium ferulate, n ‐butylidenephthalide, and ADSCs rehabilitates neurovascular unit in rats after photothrombotic stroke
AbstractThe remodelling of structural and functional neurovascular unit (NVU) becomes a central therapeutic strategy after cerebral ischaemic stroke. In the present study, we investigated the effect of combined therapy of sodium ferulate (SF),n‐butylidenephthalide (BP) and adipose‐derived stromal cells (ADSCs) to ameliorate the injured NVU in the photochemically induced thrombotic stroke in rats. After solely or combined treatment, the neovascularization, activation of astrocytes, neurogenesis, expressions of vascular endothelial grow th factor (VEGF) and claudin‐5 were assessed by immunohistochemical or immunofluore...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Yong ‐Hua Zhao,
Nai‐Wei Liu,
Chien‐Chih Ke,
Bo‐Wen Liu,
Yi‐An Chen,
Cheng Luo,
Qian Zhang,
Zhen‐Yan Xia,
Ren‐Shyan Liu Tags: ORIGINAL ARTICLE Source Type: research
CD147 promotes progression of head and neck squamous cell carcinoma via NF ‐kappa B signaling
In this study, we investigated the role of CD147 in the progression of HNSCC. Real ‐time PCR, western blot and immunohistochemistry were used to detect the expression of CD147 in total 189 HNSCC tissues in compared with normal tissues. In addition, we used proliferation, colony formation, cell cycle and apoptosis, migration and invasion as well as wound‐healing assay to determ ine the biological roles of CD147 in HNSCC. Then, a xenograft model was performed to evaluate tumor‐promoting and metastasis‐promoting role of CD147 in HNSCC. The results showed that upregulated CD147 expression was associated with aggressive...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Binbin Yu,
Yan Zhang,
Kailiu Wu,
Lizhen Wang,
Yingying Jiang,
Wantao Chen,
Ming Yan Tags: ORIGINAL ARTICLE Source Type: research
ce ‐Subpathway: Identification of ceRNA‐mediated subpathways via joint power of ceRNAs and pathway topologies
AbstractCompeting endogenous RNAs (ceRNAs) represent a novel mechanism of gene regulation that may mediate key subpathway regions and contribute to the altered activities of pathways. However, the classical methods used to identify pathways fail to specifically consider ceRNAs within the pathways and key regions impacted by them. We proposed a powerful strategy named ce ‐Subpathway for the identification of ceRNA‐mediated functional subpathways. It provided an effective level of pathway analysis via integrating ceRNAs, differentially expressed (DE) genes and their key regions within the given pathways. We respectively ...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Chenchen Feng,
Chao Song,
Ziyu Ning,
Bo Ai,
Qiuyu Wang,
Yong Xu,
Meng Li,
Xuefeng Bai,
Jianmei Zhao,
Yuejuan Liu,
Xuecang Li,
Jian Zhang,
Chunquan Li Tags: ORIGINAL ARTICLE Source Type: research
4 in 1: Antibody ‐free protocol for isolating the main hepatic cells from healthy and cirrhotic single rat livers
AbstractLiver cells isolated from pre ‐clinical models are essential tools for studying liver (patho)physiology, and also for screening new therapeutic options. We aimed at developing a new antibody‐free isolation method able to obtain the four main hepatic cell types (hepatocytes, liver sinusoidal endothelial cells [LSEC], hepatic macrophages [HMΦ] and hepatic stellate cells [HSC]) from a single rat liver. Control and cirrhotic (CCl4 and TAA) rat livers (n = 6) were perfused, digested with collagenase and mechanically disaggregated obtaining a multicellular suspension. Hepatocytes were purified by low revolution c...
Source: Journal of Cellular and Molecular Medicine - November 12, 2018 Category: Molecular Biology Authors: Anabel Fern ández‐Iglesias,
Martí Ortega‐Ribera,
Sergi Guixé‐Muntet,
Jordi Gracia‐Sancho Tags: ORIGINAL ARTICLE Source Type: research
Exploring macrophage cell therapy on Diabetic Kidney Disease
AbstractAlternatively activated macrophages (M2) have regenerative properties and shown promise as cell therapy in chronic kidney disease. However, M2 plasticity is one of the major hurdles to overcome. Our previous studies showed that genetically modified macrophages stabilized by neutrophil gelatinase ‐associated lipocalin (NGAL) were able to preserve their M2 phenotype. Nowadays, little is known about M2 macrophage effects in diabetic kidney disease (DKD). The aim of the study was to investigate the therapeutic effect of both bone marrow‐derived M2 (BM‐фM2) and ф‐NGAL macrophages in th edb/db mice. Seventeen ...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Roser Guiteras,
Anna Sola,
Maria Flaquer,
Anna Manonelles,
Georgina Hotter,
Josep M. Cruzado Tags: ORIGINAL ARTICLE Source Type: research
Benzene induces haematotoxicity by promoting deacetylation and autophagy
AbstractChronic exposure to benzene is known to be associated with haematotoxicity and the development of aplastic anaemia and leukaemia. However, the mechanism underlying benzene ‐induced haematotoxicity, especially at low concentrations of chronic benzene exposure has not been well‐elucidated. Here, we found that increased autophagy and decreased acetylation occurred in bone marrow mononuclear cells (BMMNCs) isolated from patients with chronic benzene exposure. We furth er showed in vitro that benzene metabolite, hydroquinone (HQ) could directly induce autophagy without apoptosis in BMMNCs and CD34+ cells. This was ...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Shanhu Qian,
Yixiang Han,
Yifen Shi,
Wanling Xu,
Yiyi Zhu,
Songfu Jiang,
Yi Chen,
Zhijie Yu,
Si Zhang,
Yiping Yang,
Kang Yu,
Shenghui Zhang Tags: ORIGINAL ARTICLE Source Type: research
Fisetin decreases TET1 activity and CCNY/CDK16 promoter 5hmC levels to inhibit the proliferation and invasion of renal cancer stem cell
AbstractAs a natural flavonol, fisetin has significant inhibitory effects on many cancers. Although fisetin can inhibit kidney cancer, its effects on kidney renal stem cells (HuRCSCs) remain unknown. Our study found that renal cancer tissues and CD44+/CD105+ HuRCSCs both show high TET1 protein expression. Both in vivo and in vitro experiments showed that fisetin can effectively inhibit HuRCSC cell division and proliferation, invasion, in vivo tumourigenesis and angiogenesis. Our findings showed that fisetin can significantly decrease TET1 expression levels in HuRCSCs and overall 5hmC levels in the genome s of these cel...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Yibing Si,
Junfeng Liu,
Hongliang Shen,
Chen Zhang,
Yuanhao Wu,
Yongyi Huang,
Zhangbin Gong,
Jun Xue,
Te Liu Tags: ORIGINAL ARTICLE Source Type: research
Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload
AbstractReducing sympathetic neurohormone expression is a key therapeutic option in attenuating cardiac remodelling. Present study tested the feasibility of attenuating cardiac remodelling through reducing sympathetic neurohormone level by partial cardiac sympathetic denervation in a rat model of chronic volume overload. Male Sprague ‐Dawley rats were randomized into sham group (S, n = 7), aortocaval fistula group (AV, n = 7), and aortocaval fistula with bilateral sympathetic stellate ganglionectomy group (AD, n = 8). After 12 weeks, myocardial protein expression of sympathetic neurohormones, including tyrosine hy...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Mingjing Zhang,
Pengfei Zhu,
Yuting Wang,
Jie Wu,
Yijun Yu,
Xinying Wu,
Xiaoyan Liu,
Ye Gu Tags: ORIGINAL ARTICLE Source Type: research
Gestational age ‐dependent gene expression profiling of ATP‐binding cassette transporters in the healthy human placenta
In conclusion, expressio n patterns of placental ABC transporters change as a function of gestational age. These changes are likely fundamental to a healthy pregnancy given the critical role that these transporters play in the regulation of steroidogenesis, immunological responses, and placental barrier function and integri ty. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Guinever E. Imperio,
Mohsen Javam,
Phetcharawan Lye,
Andrea Constantinof,
Caroline E. Dunk,
Fernando M. Reis,
Stephen J. Lye,
William Gibb,
Stephen G. Matthews,
Tania Maria Ortiga ‐Carvalho,
Enrrico Bloise Tags: ORIGINAL ARTICLE Source Type: research
MiR ‐520b promotes the progression of non‐small cell lung cancer through activating Hedgehog pathway
AbstractAlthough the non ‐small cell lung cancer (NSCLC) is one of the most malignant tumours worldwide, the mechanisms controlling NSCLC tumourigenesis remain unclear. Here, we find that the expression of miR‐520b is up‐regulated in NSCLC samples. Further studies have revealed that miR‐520b promotes the proliferati on and metastasis of NSCLC cells. In addition, miR‐520b activates Hedgehog (Hh) pathway. Inhibitor of Hh pathway could relieve the oncogenic effect of miR‐520b upon NSCLC cells. Mechanistically, we demonstrate that miR‐520b directly targetsSPOP 3 ′‐UTR and decreasesSPOP expression, culminating...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Xiaoming Liu,
Jichun Liu,
Xuekang Zhang,
Yuben Tong,
Xin Gan Tags: ORIGINAL ARTICLE Source Type: research
γ‐secretase inhibitor DAPT mitigates cisplatin‐induced acute kidney injury by suppressing Notch1 signaling
AbstractOrgan toxicity, including kidney injury, limits the use of cisplatin for the treatment of multiple human cancers. Hence, interventions to alleviate cisplatin ‐induced nephropathy are of benefit to cancer patients. Recent studies have demonstrated that pharmacological inhibition of the Notch signaling pathway enhances cisplatin efficacy against several cancer cells. However, whether augmentation of the anti‐cancer effect of cisplatin by Notch inhibiti on comes at the cost of increased kidney injury is unclear. We show here that treatment of mice with cisplatin resulted in a significant increase in Notch ligand D...
Source: Journal of Cellular and Molecular Medicine - November 8, 2018 Category: Molecular Biology Authors: Hitesh Soni,
Anberitha T. Matthews,
Sandeep Pallikkuth,
Rajashekhar Gangaraju,
Adebowale Adebiyi Tags: ORIGINAL ARTICLE Source Type: research
The relationship between phospholipids and insulin resistance: From clinical to experimental studies
AbstractInsulin resistance induced by high ‐fat diet and impropriate life style is a major contributor to the pathogenesis of metabolic disease. However, the underlying molecular mechanisms remain unclear. Recent studies in metabolic dysfunction have extended this beyond simply elevated cholesterol and triglycerides levels and have identif ied a key role for lipid metabolism. For example, altered phospholipid metabolism has now become central in the pathogenesis of metabolic disease. In this review, we discuss the association between insulin sensitivity and phospholipid metabolism and highlight the most significant disco...
Source: Journal of Cellular and Molecular Medicine - November 6, 2018 Category: Molecular Biology Authors: Wenguang Chang,
Grant M. Hatch,
Yu Wang,
Fei Yu,
Man Wang Tags: REVIEW Source Type: research
