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Evodiamine inhibits RANKL ‐induced osteoclastogenesis and prevents ovariectomy‐induced bone loss in mice
AbstractPostmenopausal osteoporosis (PMO) is a progressive bone disease characterized by the over ‐production and activation of osteoclasts in elderly women. In our study, we investigated the anti‐osteoclastogenic effect of evodiamine (EVO) in vivo and in vitro, as well as the underlying mechanism. By using an in vitro bone marrow macrophage (BMM)‐derived osteoclast culture system, we f ound that EVO inhibited osteoclast formation, hydroxyapatite resorption and receptor activator of NF‐κB ligand (RANKL)‐induced osteoclast marker gene and protein expression. Mechanistically, we found that EVO inhibited the deg...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Haiming Jin, Lingya Yao, Kai Chen, Yuhao Liu, Qingqing Wang, Ziyi Wang, Qian Liu, Zhen Cao, Jacob Kenny, Jennifer Tickner, Xiangyang Wang, Jiake Xu Tags: ORIGINAL ARTICLE Source Type: research

Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

The signal pathway for the repressive effect of dipyridamole on myofibroblast transdifferentiation
Journal of Cellular and Molecular Medicine, EarlyView. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Qiong Yan, Keisuke Ina, Seiichi Chiba, Huixing Wei, Shuji Tatsukawa, Yoshihisa Fujikura Tags: SHORT COMMUNICATION Source Type: research

Unveiling Ga(III) phthalocyanine —a different photosensitizer in neuroblastoma cellular model
AbstractPhthalocyanines (Pc) and their metallated derivatives are strongly considered for photodynamic therapy (PDT) possessing unique properties as possible new photosensitizers (PS). We have used toxicological assessments, real ‐time monitoring of cellular impedance, and imagistic measurements for assessing the in vitro dark toxicity and PDT efficacy of Ga(III)‐Pc in SHSy5Y neuroblastoma cells. We have established the non‐toxic concentration range of Ga(III)‐Pc, a compound which shows a high intracellular accumula tion, with perinuclear distribution in confocal microscopy. By choosing Ga(III)Pc non‐toxic dose,...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Carolina Constantin, Andreea ‐Roxana Lupu, Tudor Emanuel Fertig, Mihaela Gherghiceanu, Sevinci Pop, Rodica‐Mariana Ion, Monica Neagu Tags: ORIGINAL ARTICLE Source Type: research

Metformin relieves neuropathic pain after spinal nerve ligation via autophagy flux stimulation
In this study, we investigated the therapeutic effects of metformin on pain relief after spinal nerve ligation (SNL) and its underlying mechanism of autophagy regulation. Behavioural analysis, histological assessment, expression of c ‐Fos and molecular biological changes, as well as ultrastructural features, were investigated. Our findings showed that the number of autophagosomes and expression of autophagy markers, such as LC3 and beclin1, were increased, while the autophagy substrate protein p62, as well as the ubiquitinated proteins, were accumulated in the ipsilateral spinal cord. However, metformin enhanced the expr...
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Weidong Weng, Chenglun Yao, Keshav Poonit, Xijie Zhou, Chao Sun, Feng Zhang, Hede Yan Tags: ORIGINAL ARTICLE Source Type: research

TRPV4 regulates matrix stiffness and TGF β1‐induced epithelial‐mesenchymal transition
AbstractSubstrate stiffness (or rigidity) of the extracellular matrix has important functions in numerous pathophysiological processes including fibrosis. Emerging data support a role for both a mechanical signal, for example, matrix stiffness, and a biochemical signal, for example, transforming growth factor β1 (TGFβ1), in epithelial‐mesenchymal transition (EMT), a process critically involved in fibrosis. Here, we report evidence showing that transient receptor potential vanilloid 4 (TRPV4), a mechanosensitive channel, is the likely mediator of EMT in response to both TGFβ1 and matrix stiffness. Sp ecifically, we fou...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Shweta Sharma, Rishov Goswami, David X. Zhang, Shaik O. Rahaman Tags: ORIGINAL ARTICLE Source Type: research

Two human MARs effectively increase transgene expression in transfected CHO cells
In conclusion, MAR‐3 and MAR‐7 gene c an promote the expression of transgene in transfected CHO cells, and its effect may be related to the increase of the number of copies. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Qin Li, Chun ‐peng Zhao, Yan Lin, Chao Song, Fang Wang, Tian‐yun Wang Tags: SHORT COMMUNICATION Source Type: research

High proportion of transient neonatal zinc deficiency causing alleles in the general population
AbstractLoss of function (LoF) mutations in the zinc transporter SLC30A2/ZnT2 result in impaired zinc secretion into breast milk consequently causing transient neonatal zinc deficiency (TNZD) in exclusively breastfed infants. However, the frequency of TNZD causing alleles in the general population is yet unknown. Herein, we investigated 115 missense SLC30A2/ZnT2 mutations from the ExAC database, equally distributed in the entire coding region, harboured in 668 alleles in 60  706 healthy individuals of diverse ethnicity. To estimate the frequency of LoF SLC30A2/ZnT2 mutations in the general population, we used bioinformati...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Yarden Golan, Adrian Lehvy, Guy Horev, Yehuda G. Assaraf Tags: ORIGINAL ARTICLE Source Type: research

Engineering of TIMP ‐3 as a LAP‐fusion protein for targeting to sites of inflammation
We describe here the engineering of TIMP‐3 using a novel drug‐delivery system known as the ‘LAP technology’. This involves creatin g therapeutic proteins in fusion with the latency‐associated peptide (LAP) from the cytokine TGF‐? to generate proteins that are biologically inactive until cleavage of the LAP to release the therapy. LAP‐TIMP‐3 was successfully expressed in mammalian cells and the presence of the LAP result ed in a 14‐fold increase in the quantity of recombinant TIMP‐3 produced. LAP‐TIMP‐3 was latent until release from the LAP by treatment with matrix metalloproteinase when it could inh...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Ben M. Alberts, Sandra M. Sacre, Peter G. Bush, Lisa M. Mullen Tags: SHORT COMMUNICATION Source Type: research

Intracellular reduction in ATP levels contributes to CYT997 ‐induced suppression of metastasis of head and neck squamous carcinoma
AbstractThe incidence rate of head and neck squamous cell carcinoma (HNSCC) has steadily increased over the past decade. However, treatment options for metastatic HNSCC are often limited and the 5 ‐year survival rate has remained static. Therefore, the development and assessment of more efficient but less toxic therapeutic strategies is an unmet need for treatment of more extensive HNSCC. Here, we report that CYT997, a novel microtubule‐disrupting agent, exerts strong activity in inhibiti ng HNSCC cell invasion and metastasis. The loss of invasion capacity by CYT997 was accompanied by an associated increase in cell adh...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Xiangdong Zhao, Liwei Lang, Leilei He, Lixia Gao, David Chyan, Yuanping Xiong, Honglin Li, Hong Peng, Yong Teng Tags: ORIGINAL ARTICLE Source Type: research

Autotaxin/lysophosphatidic acid signaling mediates obesity ‐related cardiomyopathy in mice and human subjects
In conclusion, present study uncovers the correlation between circulating autotaxin and cardiac parameters in mice and human patient, and provided solid evidence of the therapeutic application of autotaxin inhibitor in combating obesity‐related cardiomyopathy. (Source: Journal of Cellular and Molecular Medicine)
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Jiakan Weng, Sheng Jiang, Lu Ding, Yi Xu, Xiongfei Zhu, Peifeng Jin Tags: ORIGINAL ARTICLE Source Type: research

Endogenous reduction of miR ‐185 accelerates cardiac function recovery in mice following myocardial infarction via targeting of cathepsin K
AbstractAngiogenesis is critical for re ‐establishing the blood supply to the surviving myocardium after myocardial infarction (MI) in patients with acute coronary syndrome (ACS). MicroRNAs are recognised as important epigenetic regulators of endothelial function. The aim of this study was to determine the roles of microRNAs in angiogen esis. Eighteen circulating microRNAs including miR‐185‐5p were differently expressed in plasma from patients with ACS by high‐throughput RNA sequencing. The expressional levels of miR‐185‐5p were dramatically reduced in hearts isolated from mice following MI and cultured human u...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Chuan ‐Chang Li, Xue‐Ting Qiu, Quan Sun, Ji‐Peng Zhou, Hui‐Jun Yang, Wan‐Zhou Wu, Ling‐Fang He, Can‐E Tang, Guo‐Gang Zhang, Yong‐Ping Bai Tags: ORIGINAL ARTICLE Source Type: research

Osterix promotes the migration and angiogenesis of breast cancer by upregulation of S100A4 expression
In this study, we demonstrated that OSX was highly expressed in metastatic breast cancer cells. Moreover, it could upregulate the expression of S100 calcium binding protein A4 (S100A4) and potentiate breast cancer cell migration and tumor angiogenesis in  vitro and in vivo. Importantly, inhibition of S100A4 impaired OSX‐induced cell migration and capillary‐like tube formation. Restored S100A4 expression rescued OSX‐short hairpin RNA‐suppressed cell migration and capillary‐like tube formation. Moreover, the expression levels of OSX and S1 00A4 correlated significantly in human breast tumors. Our study suggested ...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Shuang Qu, Jiahui Wu, Qianyi Bao, Bing Yao, Rui Duan, Xiang Chen, Lingyun Li, Hongyan Yuan, Yucui Jin, Changyan Ma Tags: ORIGINAL ARTICLE Source Type: research

Efficient method for isolation of reticulocyte RNA from healthy individuals and hemolytic anaemia patients
AbstractDespite enormous progress and development of high ‐throughput methods in genome‐wide mRNA analyses, data on the erythroid transcriptome are still limited, even though they could be useful in medical diagnostics and personalized therapy as well as in research on normal and pathological erythroid maturation. Although obtaining normal and patholog ical reticulocyte transcriptome profiles should contribute greatly to our understanding of the molecular bases of terminal erythroid differentiation as well as the mechanisms of the hematological diseases, a basic limitation of these studies is the difficulty of efficien...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Micha ł Skulski, Rafał Bartoszewski, Michał Majkowski, Beata Machnicka, Kazimierz Kuliczkowski, Aleksander F. Sikorski, Dżamila M. Bogusławska Tags: ORIGINAL ARTICLE Source Type: research

Fibroblast growth factor 21 facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagic cell death
AbstractSeeking for effective drugs which are beneficial to facilitating axonal regrowth and elongation after peripheral nerve injury (PNI) has gained extensive attention. Fibroblast growth factor 21 (FGF21) is a metabolic factor that regulates blood glucose and lipid homeostasis. However, there is little concern for the potential protective effect of FGF21 on nerve regeneration after PNI and revealing related molecular mechanisms. Here, we firstly found that exogenous FGF21 administration remarkably promoted functional and morphologic recovery in a rat model of sciatic crush injury, manifesting as persistently improved mo...
Source: Journal of Cellular and Molecular Medicine - November 18, 2018 Category: Molecular Biology Authors: Yingfeng Lu, Rui Li, Junyi Zhu, Yanqing Wu, Duohui Li, Lupeng Dong, Yiyang Li, Xin Wen, Fangzheng Yu, Hongyu Zhang, Xiao Ni, Shenghu Du, Xiaokun Li, Jian Xiao, Jian Wang Tags: ORIGINAL ARTICLE Source Type: research