Importance of the three basic residues in the vicinity of the zinc-binding motifs for the activity of the yeast dipeptidyl peptidase III
Yeast dipeptidyl peptidase III (yDPP III) is a member of the metallopeptidase family M49 involved in intracellular protein catabolism. Elucidation of the yDPP III crystal structure has pinpointed the zinc-coordinating residues (two His from H460ELLGH465 motif and the second Glu from E516ECRAE521 motif), and several amino acid residues potentially important for catalytic activity whose roles have not been investigated. Here, three putative catalytic residues of the yDPP III, His578, Arg582 and Lys638 were substituted and the resultant single mutants characterized. The replacement of His578 with an asparagine significantly (...
Source: Journal of Biochemistry - January 6, 2014 Category: Biochemistry Authors: Jajcanin-Jozic, N., Tomic, S., Abramic, M. Tags: Regular Papers Source Type: research
Imbalanced response of ATP-binding cassette transporter A1 and CD36 expression to increased oxidized low-density lipoprotein loading contributes to the development of THP-1 derived foam cells
In conclusion, the more preferentially suppressed ABCA1 expression as compared with CD36 at higher doses of oxLDL stimulation may be the initiator for the formation of macrophage-derived foam cells. (Source: Journal of Biochemistry)
Source: Journal of Biochemistry - January 6, 2014 Category: Biochemistry Authors: Liu, H.-Y., Cui, H.-B., Chen, X.-M., Chen, X.-Y., Wang, S.-H., Du, W.-P., Zhou, H.-L., Zhao, R.-C., Zhou, Y., Liu, Y.-H., Cui, C.-C., Huang, C. Tags: Regular Papers Source Type: research
Characterization of the peripheral structures of archaeal RNase P RNA from Pyrococcus horikoshii OT3
Ribonuclease P (RNase P) is a ubiquitous trans-acting ribozyme that processes the 5' leader sequence of precursor tRNA (pre-tRNA). The secondary structure of RNase P RNA (PhopRNA) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 has several peripheral stem-loops. In order to investigate their functional role, we prepared six mutants, P1, P3, P8, P9, P12 and P15, in which the stem-loops including helices P1, P3, P8, P9, P12/12.1/12.2 and P15/16 in PhopRNA were individually deleted, respectively, and characterized them with respect to pre-tRNA cleavage activity in the presence of five proteins and also to the abil...
Source: Journal of Biochemistry - January 6, 2014 Category: Biochemistry Authors: Ueda, T., Yamaguchi, H., Miyanoshita, M., Nakashima, T., Kakuta, Y., Kimura, M. Tags: Regular Papers Source Type: research
Trehalose biosynthesis in Myxococcus xanthus under osmotic stress and during spore formation
In Myxococcus xanthus, trehalose is synthesized in response to osmotic stress and during spore formation. Genome data analysis indicated that M. xanthus has five related enzymes involved in four trehalose synthesis pathways (OtsAB, TreYZ, TreT and TreS). Under osmotic conditions, the amount of trehalose in wild-type cells was increased quickly by the OtsAB pathway, and the otsAB mutant began to synthesize trehalose using the TreYZ pathway. Also, in comparison with the wild-type strain, the otsAB and treZ mutants showed ~65% and 40% reductions in the levels of intracellular trehalose accumulation, respectively, after 42 h o...
Source: Journal of Biochemistry - January 6, 2014 Category: Biochemistry Authors: Kimura, Y., Kawasaki, S., Tuchimoto, R., Tanaka, N. Tags: Regular Papers Source Type: research
Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis
In conclusion, we present the first evidence that bi-functional TilS and HprT can form a complex to function as a transcriptional activator to stimulate ftsH transcription. (Source: Journal of Biochemistry)
Source: Journal of Biochemistry - January 6, 2014 Category: Biochemistry Authors: Lin, T.-H., Hu, Y.-N., Shaw, G.-C. Tags: Regular Papers Source Type: research
