Hybridization, characterization and transferability of SSRs in the genus Morchella
Publication date: Available online 11 May 2019Source: Fungal BiologyAuthor(s): Xi-Hui Du, Hanchen Wang, Jingjing Sun, Lunyi Xiong, Jingjing YuAbstractRecently, M. importuna, M. sextelata, M. eximia, M. exuberans, Mel-13, and Mel-21 have been successfully cultivated in China and planting areas rapidly expanded because of their economic importance. Effective molecular markers are urgently needed for accurately identifying morel cultivars. Microsatellites are widely utilized for strain authentication in many fungal groups. To our knowledge, for the first time we characterized the distribution of microsatellites (simple sequen...
Source: Fungal Biology - May 13, 2019 Category: Biology Source Type: research
Interacting climate change environmental factors effects on Fusarium langsethiae growth, expression of Tri genes and T-2/HT-2 mycotoxin production on oat-based media and in stored oats
The objectives of this study were to investigate the impact that interactions between key climate change (CC) related environmental factors of temperature (20, 25, 30°C), water activity (aw; 0.995, 0.98) and CO2 exposure (400, 1000 ppm) may have on (a) growth, (b) gene expression of biosynthetic toxin genes (Tri5, Tri6, Tri16), and (c) phenotypic T-2/HT-2 production by Fusarium langsethiae on oat-based agar medium and in stored oats. Fungal growth was optimum at 25°C and 0.995 aw and reduced significantly at 30°C and intermediate stress (0.98 aw, elevated CO2 (1000 ppm) exposure by approx. 4-fold. Lag phases prior to gr...
Source: Fungal Biology - May 13, 2019 Category: Biology Source Type: research
Outcome of blue, green, red, and white light on Metarhizium robertsii during mycelial growth on conidial stress tolerance and gene expression
In this study, Metarhizium robertsii was grown on: 1) potato dextrose agar medium (PDA) in the dark (control); 2) under nutritive stress in the dark; and 3) PDA under continuous (A) white light; (B) blue light lower irradiance = LI; (C) blue light higher irradiance = HI; (D) green light; and (E) red light. Conidia produced under these treatments were tested against osmotic stress and UV radiation. In addition, a suite of genes usually involved in different stress responses were selected to study their expression patterns. Conidia produced under nutritive stress in the dark were the most tolerant to both osmotic stress and ...
Source: Fungal Biology - May 11, 2019 Category: Biology Source Type: research
Amblypygid-fungal interactions: The whip spider exoskeleton as a substrate for fungal growth
Publication date: Available online 10 May 2019Source: Fungal BiologyAuthor(s): Alastair T. Gibbons, Alexander Idnurm, Michael Seiter, Paul S. Dyer, Matthew Kokolski, Sara L. Goodacre, Stanislav N. Gorb, Jonas O. WolffAbstractFungi and arthropods represent some of the most diverse organisms on our planet, yet the ecological relationships between them remain largely unknown. In animals, fungal growth on body surfaces is often hazardous and is known to cause mortality. In contrast, here we report the presence of an apparently non-harmful mycobiome on the cuticle of whip spiders (Arachnida: Amblypygi). The associations are not...
Source: Fungal Biology - May 11, 2019 Category: Biology Source Type: research
Engineering a peptide aptamer to target calmodulin for the inhibition of Magnaporthe oryzae
Publication date: Available online 9 May 2019Source: Fungal BiologyAuthor(s): Qi Xu, Xing Ye, Xiang Ma, Hong Li, Hongqian Tang, Yanqiong Tang, Zhu LiuAbstractTo develop an antimicrobial agent for preventing the devasting damage caused by rice blast, a novel peptide aptamer was identified to interact with calmodulin (CaM) for the inhibition of the spore development in the pathogen Magnaporthe oryzae. A peptide aptamer designated as SNP-D4, consisted of the scaffold protein Staphylococcus aureus nuclease (SN) and an exposed surface loop of 16 random amino acids, was screened from the constructed peptide aptamer libraries by ...
Source: Fungal Biology - May 10, 2019 Category: Biology Source Type: research
