Evaluating the circadian rhythm and response to glucose addition in dispersed growth cultures of Neurospora crassa
Publication date: Available online 20 November 2019Source: Fungal BiologyAuthor(s): Christina M. Kelliher, Jennifer J. Loros, Jay C. DunlapAbstractWork on the filamentous fungus Neurospora crassa has contributed to or pioneered many aspects of research on circadian clock mechanism, a process that is functionally conserved across eukaryotes. Biochemical assays of the fungal circadian clock typically involve growth in liquid medium where Neurospora forms a spherical ball of submerged mycelium. Here, we revive a method for dispersed growth of Neurospora in batch culture using polyacrylic acid as an additive to the medium. We ...
Source: Fungal Biology - November 21, 2019 Category: Biology Source Type: research
Identification of two Pleurotus ostreatus blue light receptor genes (PoWC-1 and PoWC-2) and in vivo confirmation of complex PoWC-12 formation through yeast two hybrid system
In this study, we isolated homologues of the blue light receptor genes wc-1 and wc-2 from P. ostreatus, PoWC-1 and PoWC-2. The PoWC-1 contained three typical PAS domains and one PAS domain exhibited significant similarity to the LOV domain of known blue light receptors. The PoWC-2 had one typical PAS domain and one ZnF domain. The qRT-PCR analysis showed that PoWC-1 and PoWC-2 expression increased in a short time, and the final level tended to be stable along with the light illumination. The PoWC-1 and PoWC-2 expression levels of the primordium period was higher than that of mature fruiting-body period; and in the pileus ...
Source: Fungal Biology - November 16, 2019 Category: Biology Source Type: research
A combined transcriptional, biochemical and histopathological study unravels the complexity of Alternaria resistance and susceptibility in Brassica coenospecies
In this study, we have selected B. juncea (susceptible), Sinapis alba (moderately tolerant) and Camelina sativa (highly resistant) as a source for understanding and unraveling the mechanism of disease resistance against A. brassicae. Histopathological studies showed early onset of necrosis in B. juncea (1day post inoculation) and delayed in S. alba (2 dpi) and C. sativa (3 dpi) respectively. Based on the biochemical observations, early and enhanced production of hydrogen peroxide (H2O2) was observed in C. sativa and S. alba (6 hpi) when compared to B. juncea (12 hpi) after A. brassicae infection. An increase in catalase ac...
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research
Botrydial confers Botrytis cinerea the ability to antagonize soil and phyllospheric bacteria
Publication date: Available online 15 November 2019Source: Fungal BiologyAuthor(s): Paulina Vignatti, María E. Gonzalez, Edgardo C. Jofré, Hernando J. Bolívar-Anillo, Javier Moraga, Muriel Viaud, Isidro G. Collado, Fernando L. PieckenstainAbstractThe role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus demonstrating the...
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research
Editorial Board
Publication date: December 2019Source: Fungal Biology, Volume 123, Issue 12Author(s): (Source: Fungal Biology)
Source: Fungal Biology - November 15, 2019 Category: Biology Source Type: research
Radioadapted Wangiella dermatitdis Senses Radiation in its Environment in a Melanin-dependent Fashion
Publication date: Available online 12 November 2019Source: Fungal BiologyAuthor(s): Mackenzie E. Malo, Connor Frank, Ekaterina DadachovaAbstractBlack fungi have the ability to withstand varied and extreme stresses, in part due to the presence of the pigment melanin. Melanin contributes to increased structural integrity and enhanced resistance to chemical and radiation stress. These advantages impart biological benefit in the form of improved health and fitness, specifically in extreme conditions. Our goal in this study was to exploit the radiation sensing nature of melanized fungus in order to develop a radioadapted strain...
Source: Fungal Biology - November 13, 2019 Category: Biology Source Type: research
Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
This study examined the effect of (i) current and increased temperature in the alternating 11.5h dark/12.5h light cycle (15-28°C vs 18-34°C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, “velvet complex”) involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general s...
Source: Fungal Biology - November 13, 2019 Category: Biology Source Type: research
Four Tulasnella taxa associated with populations of the Australian evergreen terrestrial orchid Cryptostylis ovata
Publication date: Available online 23 October 2019Source: Fungal BiologyAuthor(s): D.Q. Nguyen, Hua Li, T.T. Tran, K. Sivasithamparam, M.G.K. Jones, S.J. WylieAbstractOf the more than 400 indigenous orchid species in Western Australia, Cryptostylis ovata is the only species that retains its leaves all year round. It exists as a terrestrial herb and occasionally as an epiphyte in forested areas. Like all terrestrial orchids, C. ovata plants associate with mycorrhizal fungi, but their identities have not previously been investigated. Fungi were isolated from pelotons in rhizomes collected from three southern and two northe...
Source: Fungal Biology - November 9, 2019 Category: Biology Source Type: research
Mitigating stress in industrial yeasts
This article will highlight the importance of furthering our understanding of key aspects of yeast stress physiology and the beneficial impact this can have more generally on enhancing industrial fungal bioprocesses. (Source: Fungal Biology)
Source: Fungal Biology - November 5, 2019 Category: Biology Source Type: research
Identification of two Pleurotus ostreatus blue light receptor genes (PoWC-1 and PoWC-2) and in vivo confirmation of complex PoWC-12 formation through yeast two hybrid system
In this study, we isolated homologues of the blue light receptor genes wc-1 and wc-2 from P. ostreatus, PoWC-1 and PoWC-2. The PoWC-1 contained three typical PAS domains and one PAS domain exhibited significant similarity to the LOV domain of known blue light receptors. The PoWC-2 had one typical PAS domain and one ZnF domain. The qRT-PCR analysis showed that PoWC-1 and PoWC-2 expression increased in a short time, and the final level tended to be stable along with the light illumination. The PoWC-1 and PoWC-2 expression levels of the primordium period was higher than that of mature fruiting-body period; and in the pileus w...
Source: Fungal Biology - November 3, 2019 Category: Biology Source Type: research
Bacterial decomposition of insects post-Metarhizium infection: Possible influence on plant growth
Publication date: Available online 15 October 2019Source: Fungal BiologyAuthor(s): Vadim Y. Kryukov, Marsel R. Kabilov, Natalya Smirnova, Oksana G. Tomilova, Maksim V. Tyurin, Yuriy B. Akhanaev, Olga V. Polenogova, Viktor P. Danilov, Saule K. Zhangissina, Tatiana Alikina, Olga N. Yaroslavtseva, Viktor V. GlupovAbstractStrains of entomopathogenic fungi may have substantial differences in their final stages of mycosis. Insect cadavers are usually overgrown with mycelium after colonization of the insect body, but in many cases, bacterial decomposition of the colonized hosts occurs. We used two Metarhizium robertsii strains in...
Source: Fungal Biology - October 31, 2019 Category: Biology Source Type: research
Crosstalk between Saccharomyces cerevisiae SAPKs Hog1 and Mpk1 is mediated by glycerol accumulation
In this study, we show that hyperactivation of Mpk1 in a ptp2 ptp3 null mutant is an indirect consequence of Hog1 hyperactivation, which induces accumulation of intracellular glycerol and an attendant hypo-osmotic stress. Mpk1 hyperactivity in the absence of PTP2 and PTP3 was suppressed by a hog1 null mutation, or by restoration of osmotic balance with a constitutive form of the glycerol channel Fps1. We found similarly that activation of Mpk1 in response to zymolyase treatment is partly a consequence of Hog1-driven glycerol accumulation. Thus, we have identified two conditions in which glycerol serves as a mediator of cro...
Source: Fungal Biology - October 29, 2019 Category: Biology Source Type: research
General stress response or adaptation to rapid growth in Aspergillus nidulans?
Publication date: Available online 28 October 2019Source: Fungal BiologyAuthor(s): Károly Antal, Barnabás Cs. Gila, István Pócsi, Tamás EmriAbstractGenome-wide transcriptional changes in Aspergillus nidulans induced by nine different stress conditions were evaluated to reveal the general environmental stress response gene set showing unidirectional expressional changes under various types of stress. Clustering the genes by their transcriptional changes was a useful technique for identifying large groups of co-regulated genes. Altogether, 1642 co-upregulated and 3916 co-downregulated genes were identified. Nevertheless...
Source: Fungal Biology - October 29, 2019 Category: Biology Source Type: research
Gene expression varies within and between enzootic and epizootic lineages of Batrachochytrium dendrobatidis (Bd) in the Americas
Publication date: Available online 24 October 2019Source: Fungal BiologyAuthor(s): C.A. McDonald, A.R. Ellison, L.F. Toledo, T.Y. James, K.R. ZamudioAbstractWhile much research focus is paid to hypervirulent fungal lineages during emerging infectious disease outbreaks, examining enzootic pathogen isolates can be equally fruitful in delineating infection dynamics and determining pathogenesis. The fungal pathogen of amphibians, Batrachochytrium dendrobatidis (Bd), exhibits markedly different patterns of disease in natural populations, where it has caused massive amphibian declines in some regions, yet persists enzootically i...
Source: Fungal Biology - October 26, 2019 Category: Biology Source Type: research
Carbon dioxide production as an indicator of Aspergillus flavus colonisation and aflatoxins/cyclopiazonic acid contamination in shelled peanuts stored under different interacting abiotic factors
This study evaluated the relationship between storage of shelled peanuts under interacting abiotic conditions on (a) the temporal colonisation kinetics and cumulative respiration rates, (b) quantified the effect on dry matter losses (DMLs) and (c) quantify accumulation of aflatoxin B1 (AFB1) and CPA. Both naturally contaminated and inoculated peanuts with A. flavus conidia were stored for 7-days under different water activity (aw; 0.77-0.95) and temperature levels (20-35°C). Gas Chromatography (GC-TCD) was used to measure the temporal respiration rates (R) and the total accumulated CO2 production. Mycotoxin contamination ...
Source: Fungal Biology - October 26, 2019 Category: Biology Source Type: research
