First person - Lisa Elmen [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Lisa Elmén is first author on ‘Silencing of CCR4-NOT complex subunits affects heart structure and function’, published in DMM. Lisa conducted the research described in this article while a lab manager/research associate in Rolf Bodmer's lab at Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA. She is now a scientist at Bloom Science, San Diego, CA, USA researching bacter...
Source: DMM Disease Models and Mechanisms - July 19, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
First person - Nagisa Yoshida [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Nagisa Yoshida is first author on ‘The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages’, published in DMM. Nagisa conducted the research described in this article while a PhD student in Prof. Serge Mostowy and Dr Eva Frickel's labs at Imperial College London/The Francis Crick Institute/London School of Hygiene and Tropical Medicine...
Source: DMM Disease Models and Mechanisms - July 19, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
Silencing of CCR4-NOT complex subunits affects heart structure and function [RESEARCH ARTICLE]
This article has an associated First Person interview with the first author of the paper. (Source: DMM Disease Models and Mechanisms)
Source: DMM Disease Models and Mechanisms - July 19, 2020 Category: Biomedical Science Authors: Elmen, L., Volpato, C. B., Kervadec, A., Pineda, S., Kalvakuri, S., Alayari, N. N., Foco, L., Pramstaller, P. P., Ocorr, K., Rossini, A., Cammarato, A., Colas, A. R., Hicks, A. A., Bodmer, R. Tags: Stem Cells, Drosophila as a Disease Model RESEARCH ARTICLE Source Type: research
The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages [RESEARCH ARTICLE]
This article has an associated First Person interview with the first author of the paper. (Source: DMM Disease Models and Mechanisms)
Source: DMM Disease Models and Mechanisms - July 19, 2020 Category: Biomedical Science Authors: Yoshida, N., Domart, M.-C., Peddie, C. J., Yakimovich, A., Mazon-Moya, M. J., Hawkins, T. A., Collinson, L., Mercer, J., Frickel, E.-M., Mostowy, S. Tags: Zebrafish as a Disease Model RESEARCH ARTICLE Source Type: research
First person - Craig Keenan [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Craig Keenan is first author on ‘Post-traumatic osteoarthritis development is not modified by postnatal chondrocyte deletion of Ccn2’, published in DMM. Craig conducted the research described in this article while a postdoctoral research associate in Dr Blandine Poulet's lab at the University of Liverpool, Liverpool, UK. He is now a lecturer in vertebrate physiology in the lab of Dr Jason Kirby at...
Source: DMM Disease Models and Mechanisms - July 13, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
Post-traumatic osteoarthritis development is not modified by postnatal chondrocyte deletion of Ccn2 [RESEARCH ARTICLE]
This article has an associated First Person interview with the first author of the paper. (Source: DMM Disease Models and Mechanisms)
Source: DMM Disease Models and Mechanisms - July 13, 2020 Category: Biomedical Science Authors: Keenan, C. M., Ramos-Mucci, L., Kanakis, I., Milner, P. I., Leask, A., Abraham, D., Bou-Gharios, G., Poulet, B. Tags: RESEARCH ARTICLE Source Type: research
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts [RESOURCE ARTICLE]
ABSTRACT
SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour...
Source: DMM Disease Models and Mechanisms - July 13, 2020 Category: Biomedical Science Authors: Krasny, L., Bland, P., Burns, J., Lima, N. C., Harrison, P. T., Pacini, L., Elms, M. L., Ning, J., Martinez, V. G., Yu, Y.-R., Acton, S. E., Ho, P.-C., Calvo, F., Swain, A., Howard, B. A., Natrajan, R. C., Huang, P. H. Tags: Cancer, Tools and Resources for Mouse Studies RESOURCE ARTICLE Source Type: research
Sentinel interaction mapping - a generic approach for the functional analysis of human disease gene variants using yeast [RESOURCE ARTICLE]
ABSTRACT
Advances in sequencing technology have led to an explosion in the number of known genetic variants of human genes. A major challenge is to now determine which of these variants contribute to diseases as a result of their effect on gene function. Here, we describe a generic approach using the yeast Saccharomyces cerevisiae to quickly develop gene-specific in vivo assays that can be used to quantify the level of function of a genetic variant. Using synthetic dosage lethality screening, ‘sentinel’ yeast strains are identified that are sensitive to overexpression of a human disease gene. Variants of the ge...
Source: DMM Disease Models and Mechanisms - July 7, 2020 Category: Biomedical Science Authors: Young, B. P., Post, K. L., Chao, J. T., Meili, F., Haas, K., Loewen, C. Tags: RESOURCE ARTICLE Source Type: research
Neuromuscular disease modeling on a chip [REVIEW]
ABSTRACT
Organs-on-chips are broadly defined as microfabricated surfaces or devices designed to engineer cells into microscale tissues with native-like features and then extract physiologically relevant readouts at scale. Because they are generally compatible with patient-derived cells, these technologies can address many of the human relevance limitations of animal models. As a result, organs-on-chips have emerged as a promising new paradigm for patient-specific disease modeling and drug development. Because neuromuscular diseases span a broad range of rare conditions with diverse etiology and complex pathophysiology, the...
Source: DMM Disease Models and Mechanisms - July 6, 2020 Category: Biomedical Science Authors: Santoso, J. W., McCain, M. L. Tags: Organoids, Neuromuscular, Model Systems in Drug Discovery REVIEW Source Type: research
First person - Dimitrije Stankovic [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Dimitrije Stanković is first author on ‘A Drosophila model to study retinitis pigmentosa pathology associated with mutations in the core splicing factor Prp8’, published in DMM. Dimitrije is a PhD student in the lab of Mirka Uhlirova at CECAD Research Center in Cologne, Germany, investigating the functional consequences of aberrant pre-mRNA splicing in the context of regulation of gene expr...
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
First person - Alec Nickolls [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Alec Nickolls is first author on ‘Human embryoid bodies as a 3D tissue model of the extracellular matrix and α-dystroglycanopathies’, published in DMM. Alec conducted the research described in this article while a PhD student in Carsten Bönnemann's lab at National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA. He is now a postdoctora...
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
First person - Sara Ibrahim [FIRST PERSON]
ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Disease Models & Mechanisms, helping early-career researchers promote themselves alongside their papers. Sara Ibrahim is first author on ‘A novel Cre-enabled tetracycline-inducible transgenic system for tissue-specific cytokine expression in the zebrafish: CETI-PIC3’, published in DMM. Sara is an MD-PhD student in the lab of Emily K. Sims and Ryan M. Anderson at Indiana University School of Medicine, Indianapolis, IN, USA, developing and utilizing molecular biology techniques to study β cell dys...
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Tags: FIRST PERSON Source Type: research
Quantitative single-cell interactomes in normal and virus-infected mouse lungs [RESOURCE ARTICLE]
ABSTRACT
Mammalian organs consist of diverse, intermixed cell types that signal to each other via ligand-receptor interactions – an interactome – to ensure development, homeostasis and injury-repair. Dissecting such intercellular interactions is facilitated by rapidly growing single-cell RNA sequencing (scRNA-seq) data; however, existing computational methods are often not readily adaptable by bench scientists without advanced programming skills. Here, we describe a quantitative intuitive algorithm, coupled with an optimized experimental protocol, to construct and compare interactomes in control and Sendai viru...
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Authors: Cain, M. P., Hernandez, B. J., Chen, J. Tags: RESOURCE ARTICLE Source Type: research
A Drosophila model to study retinitis pigmentosa pathology associated with mutations in the core splicing factor Prp8 [RESEARCH ARTICLE]
This article has an associated First Person interview with the first author of the paper. (Source: DMM Disease Models and Mechanisms)
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Authors: Stankovic, D., Claudius, A.-K., Schertel, T., Bresser, T., Uhlirova, M. Tags: Drosophila as a Disease Model RESEARCH ARTICLE Source Type: research
Human embryoid bodies as a 3D tissue model of the extracellular matrix and {alpha}-dystroglycanopathies [RESOURCE ARTICLE]
This article has an associated First Person interview with the first author of the paper. (Source: DMM Disease Models and Mechanisms)
Source: DMM Disease Models and Mechanisms - June 25, 2020 Category: Biomedical Science Authors: Nickolls, A. R., Lee, M. M., Zukosky, K., Mallon, B. S., Bönnemann, C. G. Tags: Organoids, Neuromuscular RESOURCE ARTICLE Source Type: research
