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Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion System
Investigation of secretion systems is often critical to understanding the virulence mechanisms of bacterial pathogens. With estimates as high as 30–40% of proteins secreted or localized to the cell envelope, information about the subcellular localization and organization of secretion complexes and identification and functional characterization of their substrates are key steps toward understanding these intricate systems. Here we describe a protocol using fluorescent live-cell imaging of fusion proteins that can provide a powerful tool to potentially examine the localization, assembly, and role of each component in t...
Source: Springer protocols feed by Microbiology - January 10, 2013 Category: Microbiology Source Type: news

Molecular Identification of Microbes: IV. Vibrio
Vibrios are ubiquitous and abundant in aquatic environment. A huge fraction of vibrios have also been detected in marine environments. Being of environmental origin, Vibrio species can cause the diseases like cholera and gastroenteritis. Thus detection of the species becomes utmost important when there is the need of disease diagnosis and the causative agent detection. Though there are many techniques available for the identification of Vibrio species manually, they are time-consuming procedures and not economical. Hence in this chapter, we have described the techniques of identification of Vibrio species at molecular leve...
Source: Springer protocols feed by Microbiology - January 1, 2013 Category: Microbiology Source Type: news